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Method for rapidly separating and determining water-soluble B-vitamins in infant food and dairy products

A technology for infant food and B vitamins, applied in the direction of material separation, measuring devices, and analysis of materials, can solve the problems of cumbersome steps, increase labor costs, and occupying experimental time, etc., and achieve wide linear range, good linearity, and reduced analysis the effect of time

Inactive Publication Date: 2017-05-17
天津量信检验认证技术有限公司
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, the sample pretreatment for different testing items is not only cumbersome, but also increases labor costs and takes up experimental time.

Method used

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  • Method for rapidly separating and determining water-soluble B-vitamins in infant food and dairy products
  • Method for rapidly separating and determining water-soluble B-vitamins in infant food and dairy products
  • Method for rapidly separating and determining water-soluble B-vitamins in infant food and dairy products

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Embodiment Construction

[0030] The present invention will be further described in detail below through the specific examples, the following examples are only descriptive, not restrictive, and cannot limit the protection scope of the present invention with this.

[0031] A method for rapidly separating and determining water-soluble B vitamins in food for infants and young children and dairy products, characterized in that: the method includes the following steps:

[0032] 1) Preparation of standard working solution:

[0033] Weigh an appropriate amount of vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin B9, and vitamin B12 standard products into a brown volumetric flask, dilute to 100mL with pure water, and store in a freezer. The linear range of the concentration of each standard product is shown in the following table :

[0034] Table 1 standard solution concentration range

[0035] project Linear range (μg / L) Vitamin B 1

2-100 Vitamin B 2

5-600 ...

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Abstract

The invention relates to a method for rapidly separating and determining water-soluble B-vitamins in infant food and dairy products. The method comprises the following steps: preparing a standard working solvent: weighing an appropriate amount of vitamin B1, vitamin B2, vitamin B3, vitamin B5, vitamin B6, vitamin B9 and vitamin B12 standard substances, placing into a brown volumetric flask, reaching a constant volume of 100mL by virtue of ultra-pure water, and freezing storing; preparing a sample working solvent: weighing 3 to 8 g of samples, adding 50 to 70 mL of 0.1mol / L hydrochloric acid, uniformly mixing, sealing, placing into a high-pressure sterilizing pot, keeping for 25 to 35 min at 120 to 125 DEG C, dissolving, and performing pretreatment for the sample by adopting an enzymolysis method; and analyzing by adopting a liquid-phase chromatograph-mass spectrometry. The method can analyze and detect seven water-soluble B-vitamins, ensure the recovery rate of each component and save the detection time and cost.

Description

technical field [0001] The invention belongs to the field of food detection, in particular to a method for rapidly separating and measuring water-soluble B vitamins in food for infants and young children and dairy products. Background technique [0002] B vitamins are water-soluble vitamins that are indispensable for maintaining normal functions and metabolic activities of the human body. The human body cannot synthesize them by itself and must be supplemented. Due to the limited food intake of infants and young children, the main source of their intake of nutrients is obtained through infant food and dairy products. At present, my country's determination methods for B vitamins in infant food and dairy products are mainly based on the corresponding standard methods of the national standard GB5413 series, as follows: [0003] Vitamin B1 is in accordance with the standard "GB 5413.11-2010 National Food Safety Standard Determination of Vitamin B1 in Infant Food and Dairy Produ...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 张克禄吕明明郝景雯刘然许勤虎
Owner 天津量信检验认证技术有限公司
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