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Methods and applications for assembling and immobilizing nanostructures using engineered biofilms

A nanostructure and biofilm technology, applied in nanostructure assembly, nanostructure manufacturing, nanotechnology and other directions, can solve problems such as time-consuming and laborious, difficult to ensure large-scale large-scale production, and complex micro-nano processing technology program control.

Active Publication Date: 2019-05-07
SHANGHAI TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these nanomaterial surface self-assembly methods are only suitable for specific substrates (it may not be possible to reassemble if the substrate is changed), and it is difficult for these methods to ensure the same order in a large area.
Most methods also require complex micro-nano-fabrication techniques and fine program control
In addition, the current method is difficult to achieve a good array on the three-dimensional interface
[0003] How to realize the multi-scale controlled and ordered self-assembly of nanomaterials on the solid surface or interface, and the firm and regular arrangement is the key to the application of catalysis, electronics, photons, plasmas, biosensors and various energy devices. At the same time of regular arrangement, the firm contact or attachment of the structure and the substrate material often depends on multiple technologies, which is time-consuming, laborious, expensive, and it is difficult to ensure large-scale mass production

Method used

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  • Methods and applications for assembling and immobilizing nanostructures using engineered biofilms
  • Methods and applications for assembling and immobilizing nanostructures using engineered biofilms
  • Methods and applications for assembling and immobilizing nanostructures using engineered biofilms

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0114] A method for assembling and fixing nanostructures using engineered biofilms, the specific steps are:

[0115] 1. Synthesis of nano-objects with NTA ligands:

[0116]In order to realize the modification and directional assembly of nano-objects on the surface of biological membranes, it is necessary to synthesize corresponding nano-objects with NTA ligands. The present invention provides a simple method for the preparation of large-scale NTA-modified nano-objects. Such as Figure 14 As shown, HS-NTA and nano-objects are first synthesized, and then through ligand exchange, the coordination bond between the sulfhydryl group and the metal is used to realize the connection of NTA ligands and nano-objects to obtain nano-objects with NTA ligands.

[0117] HS-NTA is used as a ligand exchange reagent in the present invention. After ligand exchange, various nanomaterials including gold nanoparticles, semiconductor nanorods, quantum dots, etc. can be modified with HS-NTA and dispe...

Embodiment 2

[0154] The assembly and immobilization of nanoscale objects on solid surfaces is of great significance for catalysis, electronics, photonics, plasmonics, biosensors, and energy-related applications. The present invention develops a one-step solution method through the co-cultivation of E. coli seed liquid and nano-objects, while utilizing the inherent adhesion of E. coli biofilms to surfaces in nature. Large-scale modification of nano-objects on various surfaces and interfaces, even internal surfaces, has been realized.

[0155] A method for assembling and fixing nanostructures using engineered biofilms, the specific steps are:

[0156] 1. Synthesize CdSeS@ZnS quantum dots with Co-NTA ligands using the same method as in Steps 1 and 2 in Example 1;

[0157] 2. Using a one-step solution method, the engineered Escherichia coli is cultivated in the culture solution containing the nano-objects with metal-NTA ligands, the culture solution is equipped with a mold, and the mold is mo...

Embodiment 3

[0163] Spatial arrangement of inorganic materials induced by light control:

[0164] In nature, light has excellent resolution, so in the process of micro-nano processing and mold processing, lasers are often used as light sources to perform these operations. Arraying is very important for how to make devices, and the method of micro-nano processing to realize arraying is very complicated. The present invention develops a simple method based on light control that can be used for arraying nanometer objects. The present invention firstly arranges simple graphics, and then gradually expands to more complex arrays.

[0165] Acquisition of pDawn plasmid:

[0166] The pDawn plasmid has the same drug resistance as the csgA knockout strain of the present invention, and cannot screen bacteria. Therefore, the first step is to transform the pDawn plasmid to knock out its kanamycin resistance or replace it with a different drug resistance for convenience. , the present invention chooses...

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Abstract

The invention provides a method of utilizing an engineering biofilm to assemble and fix a nanostructure and application of the method. The method is characterized by including: synthesizing a nano article with NTA ligand, and adding metal ions to obtain a nano article with metal-NTA ligand; culturing engineered Escherichia coli in a culture solution containing the metal-NTA ligand to obtain a biofilm modified by the nano article. The method can be used for directionally assemble and fix inorganic materials like nanoparticles, quantum dots and nanorods on the surfaces of metal, inorganic materials and polymer to realize large-scale multi-dimension nano article arrangement and can be applied in the fields of energy sources, environment and electronics.

Description

technical field [0001] The invention relates to a one-step method for self-assembling and fixing nanostructures at the interface using engineering biofilm materials, in particular to using biofilms to assemble gold nanoparticles, quantum dots and nanorods on the surface and interface of materials and fixed method. Background technique [0002] Catalyzing 1-4 ,electronic 5-7 , Photon 8 ,plasma 9-11 ,biological sensor 12-15 and energy 16-18 In related applications, it is very important to realize the controllable order and regular arrangement of nanomaterials on the solid surface or inner surface. The method of assembly should try to meet the needs of being simple, reliable, universal and capable of large-scale deployment or production 19，20 . At present, the assembly of nanomaterials on the surface can be controlled by evaporation, electric field and template 21，22 . However, these nanomaterial surface self-assembly methods are only suitable for specific substrates ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B82B3/00B82B1/00B82Y40/00B82Y5/00
CPCB82B1/001B82B3/0047B82Y5/00B82Y40/00
Inventor 钟超王新宇蒲嘉华安柏霖
Owner SHANGHAI TECH UNIV