Method for removing antibiotics resistance gene in sewage

A technology of antibiotic resistance and genetics, applied in chemical instruments and methods, multi-stage water/sewage treatment, biological water/sewage treatment, etc., can solve problems such as poor treatment effect, high treatment cost, complex operation management, etc., to achieve Save operation and management costs, simple operation and reduce secondary pollution

Active Publication Date: 2017-05-24
TONGJI UNIV
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Problems solved by technology

This method solves the problems of poor treatment effect, high treatment cost and complicated operation and management in the current sewage purification treatment process for the removal of ARGs.

Method used

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  • Method for removing antibiotics resistance gene in sewage
  • Method for removing antibiotics resistance gene in sewage
  • Method for removing antibiotics resistance gene in sewage

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Embodiment Construction

[0021] A, Construct the anoxic / aerobic-membrane bioreactor (A / O-MBR) of the present invention, and the operating conditions of the A / O-MBR reactor are shown in Table 1;

[0022] Table 1 A / O-MBR operating conditions

[0023]

[0024] The A / O-MBR was inoculated with activated sludge from the aeration tank of Shanghai Quyang Wastewater Treatment Plant. The basic properties of the inoculated sludge are shown in Table 2.

[0025] Table 2 Basic properties of inoculated sludge

[0026]

[0027]The influent is artificially prepared to simulate urban sewage (the relative abundance of ARGs to be measured in the influent is 1.11 copy number / 16SrRNA sequence number), and the A / O is stabilized by adjusting operating parameters such as aeration flow, membrane flux, and solid residence time -MBR running status;

[0028] In this embodiment, artificially synthesized simulated urban domestic sewage is used as the purification object (the relative abundance of ARGs to be measured in the ...

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Abstract

The invention relates to a method for removing antibiotics resistance gene in sewage, and belongs to the technical field of sewage purification treatment. According to the method, the relevance between membrane surface pollutant components and the antibiotics resistance gene is inspected, a biological treatment and membrane technology combined process is adopted, biological treatment, membrane technology and membrane surface pollutant adsorption intercepting effects are organically coupled, adjustment and control and operating parameters during a membrane bioreactor biological process are fixed and optimized, and the optimum concentration of membrane surface organic pollutant required by the enhanced removal of the antibiotics resistance gene in the sewage is fixed, so that removal rate of the antibiotics resistance gene in the sewage reaches 93.7 percent. The method has the advantages that chemical agents are not required to be additional cast, secondary pollution is reduced, operation management costs are reduced, the significant requirements on economical, social and environmental sustainable development are met, the technology is simple and reasonable, industrialized application requirements on sewage advanced treatment and recycling are met, and a scientific and reliable novel technology is provided for controlling the spreading of the antibiotics resistance gene in the sewage.

Description

technical field [0001] The invention relates to a method for removing antibiotic resistance genes in sewage. The invention belongs to the technical field of sewage purification treatment. Background technique [0002] At present, the abuse of antibiotics in the medical, aquaculture and pharmaceutical industries has been increasing, resulting in bacterial resistance to antibiotics, which has brought great difficulties to the prevention, control and treatment of bacterial diseases. Antibiotic resistance genes (ARGs), as a new type of environmental pollutant, are one of the important factors for bacteria to acquire resistance. The generation of ARGs mainly comes from the induced mutation and horizontal transfer of genes. Mutagens in the environment, such as antibiotics and heavy metals, can induce bacterial mutations to produce ARGs, and such resistant bacteria that acquire resistance will proliferate and spread among different environmental media. ARGs carried by resistant b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/12C02F3/30C02F3/34C02F9/14C02F103/34
CPCC02F3/1268C02F3/301C02F3/34C02F9/00C02F2103/343C02F2301/04C02F2303/16Y02W10/10
Inventor 王亚宜朱奕静蒋勗欣张姚
Owner TONGJI UNIV
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