A method for constructing anode biofilm of microbial electrochemical system

Abstract

The invention provides a construction method of a microorganism electrochemical system anode biological membrane and belongs to the technical field of construction methods of anode biological membranes. The construction method comprises the following steps: directionally expanding culture on electrochemical active bacteria, collecting a microorganism sample on an anode in a bio-electrochemical reactor running steadily as an inoculation bacterium source, inoculating into a culture medium, introducing nitrogen for creating an anaerobic environment, then sealing, and culturing on a shaking table under anaerobic condition; mixing and fixing conducting particles with electrochemical active bacteria, preparing conducting particle solution, carrying out ultrasonic dispersion, then sucking a certain amount of conducting particle solution into electrochemical active bacteria solution, shaking and standing; and carrying out a filtration fixation process on the surface of an anode by a composite structure biological film. The construction method provided by the invention has the advantages that starting time and starting cycle of a biological electrochemical system are successfully shortened by constructing a composite biological film of conducting particles, electricity generation performance is greatly improved, and coulombic efficiency of the whole biological electrochemical system is also improved.

Description

technical field [0001] The invention relates to a method for constructing an anode biofilm of a microbial electrochemical system, and belongs to the technical field of anode biofilm construction methods. Background technique [0002] In the 21st century, the development direction of sewage treatment technology has gradually shifted to realizing resource utilization and energy utilization while pollutants are degraded. First of all, the current sewage treatment technology is still dominated by aerobic sewage treatment technology with high energy consumption. The treatment process needs to consume a lot of electric energy, and the consumption of electric energy will lead to the consumption of a large amount of fossil fuels, including a large amount of greenhouse gases such as carbon dioxide. emissions, exacerbating environmental problems such as global warming. Therefore, realizing the low energy consumption operation of sewage treatment has become a constant theme in the fie...

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Problems solved by technology

[0004] However, the current method still faces the following problems: a. the start-up period is long

The successful start-up of the bioelectrochemical system requires the enrichment and growth of electrogenic microorganisms on the anode surface of the reactor. However, the enrichment and acclimation process often takes a long time, generally up to 10-15 days or even longer; b. Inoculation source Instability, which is also an important factor affecting the stable start of the anode of the bioelectrochemical system, because the quality of the inoculum cannot be guaranteed whether using the anode water of the bioelectrochemical system that has been operating normally or directly using sewage inoculation, which increases the success of the anode. Uncertainty in starting c. With the gradual formation of anodic biofilm and the gradual increase in thickness, the improvement of the performance of anode biofilm will be limited, and the efficiency of electricity production and substrate degradation will reach a plateau period, which is mainly due to The restriction of the biofilm on the extracellular electron transfer process and the substrate mass transfer process in the thickness direction is caused by

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