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Lymphocyte extraction device and extraction method

A lymphocyte and extraction device technology, applied in cell dissociation methods, animal cells, sterilization methods, etc., can solve problems such as low work efficiency, pollution, cumbersome and time-consuming operations, and save time, reduce use, and avoid contact. Effect

Inactive Publication Date: 2017-05-24
LIUZHOU CITY HEALTHCARE HOSPITAL FOR WOMEN & CHILDREN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional method is cumbersome and time-consuming, and the cells need to be transferred multiple times between different instruments and centrifuged multiple times. The process is easy to cause cell loss, damage and pollution.
Due to the complex and cumbersome operation, there are shortcomings such as less extraction of cells and low extraction efficiency.

Method used

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  • Lymphocyte extraction device and extraction method
  • Lymphocyte extraction device and extraction method
  • Lymphocyte extraction device and extraction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1: a kind of lymphocyte extraction device, such as Figure 1-Figure 2 As shown, it includes a syringe 1, a separation liquid displacer 2 and a filter washing device 3. The syringe 1 is composed of an outer cylinder 12, a handle 11, a piston 13 and a needle 14. Between the handle and the piston, the outer cylinder The body and the needle can be connected in a detachable manner. The separation liquid displacer 2 is a cylinder structure with a closed lower end and an open upper end. The separation liquid displacer 2 is composed of a liquid storage tank 21 and a connecting section 22. The separation liquid The connection section 22 of the displacer and the outer cylinder can be connected in a detachable manner. The filter and washing device 3 includes a connection cylinder 31, a liquid storage cylinder 33 arranged at the lower end of the connection cylinder and a mixed fiber placed in the connection cylinder. Membrane filter membrane (MCE) 32 with a pore size of ...

Embodiment 2

[0025] Embodiment 2: a kind of lymphocyte extraction device, such as image 3 As shown, it includes a syringe 1, a separation liquid displacer 2 and a filter washing device 3, and its basic structure is the same as that of Example 1, the difference is that the liquid storage tank 21 of the separation liquid displacer 2 is also filled with lymph For the cell separation liquid P2, the upper opening of the separation liquid displacer 2 is sealed by a parafilm 4 . When in use, the sealing film 4 can be used directly after being uncovered or pierced, without adding the lymphocyte separation solution P2 inside.

Embodiment 3

[0026] Embodiment 3: a kind of lymphocyte extraction method, such as Figure 4-Figure 5 As shown, using the lymphocyte extraction device as described in Example 1, the specific operation steps are as follows:

[0027] (1) Use the syringe 1 to collect the sample peripheral blood P1, and collect the sample into the outer cylinder 12;

[0028] (2) Remove the handle 11 and needle 14 of the syringe, add the lymphocyte separation liquid P2 to the separation liquid replacer 2, the volume ratio of the amount of the lymphocyte separation liquid to the sample is 1:1, and then add the separation liquid The displacer is installed on the outer cylinder, and then centrifuged, so that the liquid in the outer cylinder is layered into serum P3, mononuclear cell layer P4 and lymphocyte separation liquid P2 from top to bottom. The liquid in the separation liquid displacer is separated from From top to bottom are lymphocyte separation solution P2 and red blood cell P5,

[0029] (3) Remove the s...

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Abstract

The invention relates to a lymphocyte extraction device and an extraction method. The lymphocyte extraction device comprises an injector, a separating medium displacer and a filter device, wherein the injector consists of an outer tube body, a pull handle, a piston and a needle head; the connection section of the separating medium displacer is detachably connected with the outer tube body; the filter device comprises a connection tube, a liquid storage tube and a mixed cellulose filter membrane placed in the connection tube; a liquid inlet is formed in the bottom of the liquid storage tube and is connected with a liquid suction pipe, and a liquid outlet is formed in the side wall of the liquid storage tube and is connected with a liquid discharging pipe; check valves are arranged at the liquid inlet and the liquid outlet; and the upper end of the connection tube is detachably connected with the outer tube body. According to the extraction method implemented through the device, cells to be extracted can be always retained in the original container, and unnecessary cells and plasma can be easily and conveniently removed, so that time and labor for cell separation and extraction are greatly saved, the working efficiency is high, more cells with higher purity are extracted, and the possibility of pollution is lower.

Description

technical field [0001] The invention relates to a lymphocyte extraction device and an extraction method. Background technique [0002] Lymphocyte separation and extraction is a preliminary cell purification method commonly used in clinical examination and scientific research. The basic principle is density gradient centrifugation. The traditional method is to add the initial sample to the lymphocyte separation medium and centrifuge, and then absorb a single nucleus after centrifugation. The cell suspension layer was finally added with saline and washed repeatedly by centrifugation. The traditional method is cumbersome and time-consuming, and the cells need to be transferred multiple times between different instruments and centrifuged multiple times. The process is easy to cause cell loss, damage and pollution. Due to the complex and cumbersome operation, there are shortcomings such as a small amount of cell extraction and low extraction efficiency. Contents of the inventi...

Claims

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Application Information

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IPC IPC(8): C12M1/12C12N5/078
CPCC12M47/04C12N5/0634C12N2509/00
Inventor 黄诚
Owner LIUZHOU CITY HEALTHCARE HOSPITAL FOR WOMEN & CHILDREN
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