Lymphocyte extraction device and extraction method

[0023] Example 1: A lymphocyte extraction device, such as Figure 1-Figure 2 As shown, it includes a syringe 1, a separating liquid displacer 2 and a filter and washing device 3. The syringe 1 is composed of an outer cylinder 12, a handle 11, a piston 13 and a needle 14, between the handle and the piston, the outer cylinder The body and the needle can be connected in a detachable manner. The separation liquid displacer 2 is a cylindrical structure with a closed lower end and an open upper end. The separation liquid displacer 2 is composed of a liquid storage tank 21 and a connecting section 22. The connecting section 22 of the displacer can be connected to the outer cylinder in a detachable manner. The filtering device 3 includes a connecting cylinder 31, a liquid storage cylinder 33 arranged at the lower end of the connecting cylinder, and a mixed fiber placed in the connecting cylinder. The element filter membrane (MCE) 32, the pore size is 0.5-2μm, the bottom of the liquid storage cylinder is provided with a liquid inlet and connected with a suction pipe 34, and the side wall of the liquid storage cylinder is provided with a liquid outlet and connected with a liquid outlet pipe 35, connected The upper end of the cylinder and the outer cylinder can be connected in a detachable manner. Both the liquid inlet and the liquid outlet are provided with check valves 36. The check valve is a one-way valve. The valve only opens in the direction of liquid flow to prevent liquid Backflow.

[0024] In this embodiment, the outer cylinder is provided with an external thread, and the needle, the upper opening of the separation liquid displacer and the upper end of the connecting cylinder are all provided with an internal thread that matches the external thread on the outer cylinder. The handle and the piston are connected by thread. As a variation, the needle, the upper opening of the separating liquid displacer and the upper end of the connecting cylinder, and the handle and the piston can also be connected by snaps or other detachable methods.

[0025] Example 2: A lymphocyte extraction device, such as image 3 As shown, it includes a syringe 1, a separated liquid displacer 2 and a filter and washing device 3. The basic structure is the same as that of Embodiment 1, except that the liquid storage tank 21 of the separated liquid displacer 2 is also filled with lymph. The cell separation liquid P2 and the upper opening of the separation liquid displacer 2 are sealed by a sealing film 4. When in use, it can be used directly by uncovering or piercing the sealing film 4, without adding the lymphocyte separation liquid P2.

[0026] Example 3: A lymphocyte extraction method, such as Figure 4-Figure 5 As shown, using the lymphocyte extraction device as described in Example 1, the specific operation steps are as follows:

[0027] (1) Use the syringe 1 to collect the sample peripheral blood P1, and collect the sample into the outer cylinder 12;

[0028] (2) Remove the handle 11 and needle 14 of the syringe, add the lymphocyte separation liquid P2 to the separation liquid displacer 2, and the volume ratio of the lymphocyte separation liquid to the sample volume is 1:1, and then the separation liquid The displacer is installed on the outer cylinder, and then centrifuged to separate the liquid in the outer cylinder from top to bottom into serum P3, mononuclear cell layer P4 and lymphocyte separation liquid P2. The liquid in the separation liquid displacer is separated from From top to bottom are lymphocyte separation solution P2 and red blood cell P5,

[0029] (3) Remove the separation liquid displacer 2, connect the handle 11 to the piston 13, then install the filter device 3 on the outer cylinder 12, and then pull the handle upward from the suction pipe 34 of the filter device 3 Inhale the physiological saline P6 to wash the liquid in the outer cylinder (traditional conventional washing is to centrifuge to remove the supernatant and add the physiological saline to resuspend and repeat twice. In this example, only the physiological saline is pumped twice, without centrifugation or mixing. ), press down the pull handle to drain the serum from the drain tube 36 of the filter and wash device, and the mononuclear cell liquid remains in the outer cylinder to complete a washing process; then repeatedly squeeze the physiological saline to carry out the liquid in the outer cylinder. After washing, the liquid finally obtained in the outer cylinder is the washing cell suspension that needs to be separated finally.

[0030] Under normal circumstances, the present invention can wash away most of the serum and remaining separated liquid by washing three times with normal saline, but if you encounter jaundice or lipemia specimens, you can increase the number of washings appropriately, which can be based on the discharge from the outlet of the drain pipe 36 To judge the turbidity and color of the saline, if the discharged saline is clean, it means that most of the impurities have been washed away.

[0031] As a variation of Embodiment 3, the lymphocyte extraction device as described in Embodiment 2 can also be used. When in use, the sealing film 4 of the separation liquid displacer 2 can be directly uncovered or pierced for use, and there is no need to add the lymphocyte separation liquid P2 into the separation liquid displacer 2.