Lymphocyte extraction device and extraction method

A lymphocyte and extraction device technology, applied in cell dissociation methods, animal cells, sterilization methods, etc., can solve problems such as low work efficiency, pollution, cumbersome and time-consuming operations, and save time, reduce use, and avoid contact. Effect

Inactive Publication Date: 2017-05-24
LIUZHOU CITY HEALTHCARE HOSPITAL FOR WOMEN & CHILDREN
View PDF0 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional method is cumbersome and time-consuming, and the cells need to be transferred multiple times between different instruments and centrifuged multiple times. The proces

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Lymphocyte extraction device and extraction method
  • Lymphocyte extraction device and extraction method
  • Lymphocyte extraction device and extraction method

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Example 1: A lymphocyte extraction device, such as Figure 1-Figure 2 As shown, it includes a syringe 1, a separating liquid displacer 2 and a filter and washing device 3. The syringe 1 is composed of an outer cylinder 12, a handle 11, a piston 13 and a needle 14, between the handle and the piston, the outer cylinder The body and the needle can be connected in a detachable manner. The separation liquid displacer 2 is a cylindrical structure with a closed lower end and an open upper end. The separation liquid displacer 2 is composed of a liquid storage tank 21 and a connecting section 22. The connecting section 22 of the displacer can be connected to the outer cylinder in a detachable manner. The filtering device 3 includes a connecting cylinder 31, a liquid storage cylinder 33 arranged at the lower end of the connecting cylinder, and a mixed fiber placed in the connecting cylinder. The element filter membrane (MCE) 32, the pore size is 0.5-2μm, the bottom of the liquid st...

Example Embodiment

[0025] Example 2: A lymphocyte extraction device, such as image 3 As shown, it includes a syringe 1, a separated liquid displacer 2 and a filter and washing device 3. The basic structure is the same as that of Embodiment 1, except that the liquid storage tank 21 of the separated liquid displacer 2 is also filled with lymph. The cell separation liquid P2 and the upper opening of the separation liquid displacer 2 are sealed by a sealing film 4. When in use, it can be used directly by uncovering or piercing the sealing film 4, without adding the lymphocyte separation liquid P2.

Example Embodiment

[0026] Example 3: A lymphocyte extraction method, such as Figure 4-Figure 5 As shown, using the lymphocyte extraction device as described in Example 1, the specific operation steps are as follows:

[0027] (1) Use the syringe 1 to collect the sample peripheral blood P1, and collect the sample into the outer cylinder 12;

[0028] (2) Remove the handle 11 and needle 14 of the syringe, add the lymphocyte separation liquid P2 to the separation liquid displacer 2, and the volume ratio of the lymphocyte separation liquid to the sample volume is 1:1, and then the separation liquid The displacer is installed on the outer cylinder, and then centrifuged to separate the liquid in the outer cylinder from top to bottom into serum P3, mononuclear cell layer P4 and lymphocyte separation liquid P2. The liquid in the separation liquid displacer is separated from From top to bottom are lymphocyte separation solution P2 and red blood cell P5,

[0029] (3) Remove the separation liquid displacer 2, co...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Apertureaaaaaaaaaa
Login to view more

Abstract

The invention relates to a lymphocyte extraction device and an extraction method. The lymphocyte extraction device comprises an injector, a separating medium displacer and a filter device, wherein the injector consists of an outer tube body, a pull handle, a piston and a needle head; the connection section of the separating medium displacer is detachably connected with the outer tube body; the filter device comprises a connection tube, a liquid storage tube and a mixed cellulose filter membrane placed in the connection tube; a liquid inlet is formed in the bottom of the liquid storage tube and is connected with a liquid suction pipe, and a liquid outlet is formed in the side wall of the liquid storage tube and is connected with a liquid discharging pipe; check valves are arranged at the liquid inlet and the liquid outlet; and the upper end of the connection tube is detachably connected with the outer tube body. According to the extraction method implemented through the device, cells to be extracted can be always retained in the original container, and unnecessary cells and plasma can be easily and conveniently removed, so that time and labor for cell separation and extraction are greatly saved, the working efficiency is high, more cells with higher purity are extracted, and the possibility of pollution is lower.

Description

technical field [0001] The invention relates to a lymphocyte extraction device and an extraction method. Background technique [0002] Lymphocyte separation and extraction is a preliminary cell purification method commonly used in clinical examination and scientific research. The basic principle is density gradient centrifugation. The traditional method is to add the initial sample to the lymphocyte separation medium and centrifuge, and then absorb a single nucleus after centrifugation. The cell suspension layer was finally added with saline and washed repeatedly by centrifugation. The traditional method is cumbersome and time-consuming, and the cells need to be transferred multiple times between different instruments and centrifuged multiple times. The process is easy to cause cell loss, damage and pollution. Due to the complex and cumbersome operation, there are shortcomings such as a small amount of cell extraction and low extraction efficiency. Contents of the inventi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12M1/12C12N5/078
CPCC12M47/04C12N5/0634C12N2509/00
Inventor 黄诚
Owner LIUZHOU CITY HEALTHCARE HOSPITAL FOR WOMEN & CHILDREN
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap