Method for fermenting temperature-sensitive strains by phosphoric acid to produce glutamic acid
A glutamic acid and temperature-sensitive technology, which is used in the field of temperature-sensitive strains to ferment glutamic acid and phosphoric acid, can solve the problems of uneven mixing of fermentation medium, shorten the preparation time of fermentation medium, etc., so as to improve acid production. rate and sugar-acid conversion rate, shortening the preparation time, shortening the effect of the fermentation cycle
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Embodiment 1
[0047] A method for using phosphoric acid to ferment and produce glutamic acid by thermosensitive strains, the process steps and conditions of its fermentation culture are as follows:
[0048] (1) Ingredients: According to the proportioning ratio, weigh various raw materials well and dissolve them completely to make 6L fermentation medium;
[0049] The raw material ratio of the fermentation medium is as follows: hydrolyzed sugar 55-60g / L, corn steep liquor 20-25g / L, molasses 15-18g / L, soybean meal hydrolyzate 1.0-2.1g / L, H 3 PO4 0.8~3g / L, MgSO4 7H2O 1~2.1g / L, MnSO4 7H2O 0.008~0.01g / L, FeSO4 7H2O 0.055~0.085g / L, KCl 5~7g / L, threonine 0.08 ~0.15g / L, antifoaming agent 0.031~0.035g / L, neutralized with sodium hydroxide to adjust the pH of the fermentation medium to 6.8~7.0.
[0050] (2) Sterilization: Sterilize the prepared fermentation medium at a temperature of 108-110°C for 8-10 minutes, cool the sterilized medium to 33°C in the fermentation and wait for inoculation;
[0051] ...
Embodiment 2
[0057] A method for using phosphoric acid to ferment and produce glutamic acid by thermosensitive strains, the process steps and conditions of its fermentation culture are as follows:
[0058] (1) Ingredients: According to the proportioning ratio, weigh various raw materials well and dissolve them completely to make 6L fermentation medium;
[0059] The raw material ratio of the fermentation medium is as follows: hydrolyzed sugar 55-60g / L, corn steep liquor 20-25g / L, molasses 15-18g / L, soybean meal hydrolyzate 1.0-2.1g / L, H 3 PO4 0.8~3g / L, MgSO4 7H2O 1~2.1g / L, MnSO4 7H2O 0.008~0.01g / L, FeSO4 7H2O 0.055~0.085g / L, KCl 5~7g / L, threonine 0.08 ~0.15g / L, defoamer 0.031~0.035g / L, neutralized with sodium carbonate to adjust the pH of the fermentation medium to 6.8~7.0.
[0060] (2) Sterilization: Sterilize the prepared fermentation medium at a temperature of 108-110°C for 8-10 minutes, cool the sterilized medium to 33°C in the fermentation and wait for inoculation;
[0061] (3) the s...
Embodiment 3
[0067] A method for using phosphoric acid to ferment and produce glutamic acid by thermosensitive strains, the process steps and conditions of its fermentation culture are as follows:
[0068] (1) Ingredients: According to the proportioning ratio, weigh various raw materials well and dissolve them completely to make 6L fermentation medium;
[0069] The raw material ratio of the fermentation medium is as follows: hydrolyzed sugar 50-65g / L, corn steep liquor 15-30g / L, molasses 10-18g / L, soybean meal hydrolyzate 1.0-2.1g / L, H 3 PO 4 0.8~4g / L, MgSO 4 ·7H 2 O 1.0~2.1g / L, MnSO 4 ·7H 2 O0.064~0.01g / L, FeSO 4 ·7H 2 O 0.055~0.085g / L, KCl 2~7g / L, threonine 0.08~0.15g / L, defoamer 0.031~0.035g / L, use ammonia water to neutralize phosphoric acid to make the pH of the fermentation medium 6.5~7.2 .
[0070] (2) Sterilization: Sterilize the prepared fermentation medium at a temperature of 108-110°C for 8-10 minutes, cool the sterilized medium to 33°C in the fermentation and wait for in...
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