Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Porcine epidemic diarrhea virus variant inactivated vaccine and application thereof

A porcine epidemic diarrhea and inactivated vaccine technology, applied in the direction of viruses, antiviral agents, virus antigen components, etc., can solve the problems of lack of immunogenicity and achieve good immunogenicity, high antibody titer, and resistance to attack

Inactive Publication Date: 2017-05-31
BEIJING DAWEIJIA BIOTECH SHARE CO LTD
View PDF3 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The vaccines used abroad are mainly Korean attenuated strains. Domestically, they are mainly porcine epidemic diarrhea and porcine transmissible gastroenteritis dual inactivated vaccines developed by Harbin Veterinary Research Institute. The strain used in this vaccine is porcine epidemic diarrhea virus. PEDV CV777 strain, which was isolated earlier, lacks extensive immunogenicity compared with the new mutant strain, so it is necessary to screen new strains and prepare porcine epidemic diarrhea vaccine for preventing porcine epidemic diarrhea

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Porcine epidemic diarrhea virus variant inactivated vaccine and application thereof
  • Porcine epidemic diarrhea virus variant inactivated vaccine and application thereof
  • Porcine epidemic diarrhea virus variant inactivated vaccine and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1: Screening of porcine epidemic diarrhea virus PEDV / CH / BJ / 2014 strain

[0013] 1 Isolation and identification of porcine epidemic diarrhea virus PEDV / CH / BJ / 2014 strain

[0014] During the epidemiological investigation, a strain of porcine epidemic diarrhea virus (named PEDV / CH / BJ / 2014 strain) was isolated from the small intestine contents of piglets with diarrhea submitted to a pig farm in Beijing. Porcine epidemic diarrhea virus RT- The PCR test was positive, and the 2nd generation of blind passage had cytopathic changes, and regular and stable cytopathic changes appeared in the 3rd generation, and the virus content could reach 5×10 in the 5th generation. 5 TCID 50 / mL, which can be neutralized by anti-porcine epidemic diarrhea virus-specific serum, the porcine epidemic diarrhea virus PEDV / CH / BJ / 2014 strain was preserved at No. 1 Beichen West Road, Chaoyang District, Beijing on December 4, 2014 No. 3 General Microbiology Center of Chinese Academy of Scien...

Embodiment 2

[0028] Example 2 Safety Test of Porcine Epidemic Diarrhea Inactivated Vaccine

[0029] 1 Experimental method

[0030] Overdose test on pregnant sows 12 pregnant sows that were negative for porcine epidemic diarrhea neutralizing antibodies and antigens 4-6 weeks before delivery were randomly divided into 3 groups. Groups 1, 2 and 3 were injected intramuscularly with 201601 , 201602, 201603 batches of porcine epidemic diarrhea inactivated vaccines each had 2 parts per head, and the fourth group was not injected as the control group, and the sows were observed until the sows gave birth.

[0031] 2 results

[0032] Results of the overdose test Compared with the control group, the 9 pregnant sows in the 3 batches of vaccine immunization groups had no abnormalities in food intake, drinking water, pregnancy and litter, and no adverse reactions were found at the injection site.

Embodiment 3

[0033] Example 3 Efficacy Test of Porcine Epidemic Diarrhea Inactivated Vaccine

[0034] 1 Experimental method

[0035] Fifteen pregnant sows at the same pre-delivery date who were negative for neutralizing antibodies and antigens in porcine epidemic diarrhea 5 weeks before delivery were randomly divided into 5 groups with 3 heads in each group. Group 1 intramuscular injection of 201601 batches of porcine epidemic diarrhea virus inactivated vaccine 1 head / head; group 2 intramuscular injection of 201602 batches of porcine epidemic diarrhea virus inactivated vaccine 1 head / head; group 3 intramuscular injection of 201603 batches of pigs Epidemic diarrhea virus inactivated vaccine 1 head / head; Group 4 intramuscular injection of commercial porcine transmissible gastroenteritis-porcine epidemic diarrhea dual inactivated vaccine 1 head / head; Group 5 muscle sterile cell culture Liquid DMEM 4 mL / head served as the control group.

[0036] After the sows gave birth, 15 3-day-old piglet...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a porcine epidemic diarrhea virus variant inactivated vaccine which is prepared after inactivating a diarrhea virus variant PEDV / CH / BJ / 2014. The vaccine is excellent in immunogenicity; after a sow is inoculated with the vaccine before parturition, a neutralizing antibody is rapidly produced, the antibody titer is high, the maintenance time is long, a piglet of the inoculated sow litter acquires better passive immunity, the piglet is effectively protected against the attack of the epidemic diarrhea virus, and the survival rate of the piglet is increased.

Description

technical field [0001] The invention belongs to the field of veterinary biological products, and in particular relates to an inactivated vaccine of porcine epidemic diarrhea virus mutant strain and application thereof. Background technique [0002] Porcine Epidemic Diarrhea Virus (PEDV) is a member of the Coronaviridae family of Coronaviridae, which can cause intestinal infectious diseases of pigs characterized by vomiting, diarrhea and dehydration. Pigs of all ages are susceptible, but suckling piglets are the most affected, with mortality rates as high as 100%. In recent years, the outbreak of PED in many provinces in my country has caused serious economic losses to the pig industry. At present, there is no specific drug to treat porcine epidemic diarrhea, and the prevention and control of the disease is mainly based on vaccination. The vaccines used abroad are mainly Korean attenuated strains. Domestically, they are mainly porcine epidemic diarrhea and porcine transmiss...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/215A61P31/14C12N7/00C12N7/04
CPCA61K39/12A61K2039/5252A61K2039/55566C12N7/00C12N2770/20034
Inventor 张美美金忠辉闫雷张晓杰侯艳红李爽乔琳
Owner BEIJING DAWEIJIA BIOTECH SHARE CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com