Preparation method of hyaluronic acid blended nanofiber immobilized enzyme

A nanofiber, hyaluronic acid technology, applied in the direction of fixing on/in organic carriers, fiber processing, fiber chemical characteristics, etc., can solve the problem of providing a mild microenvironment, easy shedding of modified materials, and poor biocompatibility and other problems to achieve the effect of improving biocompatibility and water retention, good water retention performance and high porosity

Inactive Publication Date: 2017-05-31
ANHUI UNIVERSITY OF TECHNOLOGY AND SCIENCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Nanofibers are prepared by electrospinning technology. On the one hand, the current enzyme immobilization carrier uses ordinary polymers as the matrix. Such matrix has poor biocompatibility and it is difficult to provide a mild microenvironment for the enzyme.
On the other hand, biocompatible materials such as chitosan are grafted to modify the surface of nanofibers by adsorption or covalent bonds; the steps of this method are cumbersome, and the grafting rate is low due to the large steric hindrance; The adsorbed modified material is easy to fall off

Method used

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  • Preparation method of hyaluronic acid blended nanofiber immobilized enzyme
  • Preparation method of hyaluronic acid blended nanofiber immobilized enzyme
  • Preparation method of hyaluronic acid blended nanofiber immobilized enzyme

Examples

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Embodiment 1

[0039] A preparation method for immobilizing enzymes in hyaluronic acid blended nanofibers, comprising the following steps:

[0040] (1) Configuration of hyaluronic acid solution

[0041] Measure 1.5mL of DMF solution and 1.5mL of distilled water into a test tube, weigh 4mg of sodium hyaluronate solid with an electronic balance, add it into it and dissolve to obtain a sodium hyaluronate solution.

[0042] (2) Configuration of PMA-AA binary interpolymer

[0043]Dissolve 2mL of acrylic acid and 14mL of methyl acrylate in 40mL of N,N-dimethylformamide (DMF), add 0.2-0.3g of azobisisopropionitrile, fill with nitrogen gas for 10-12min, and place at 60-80°C Heat in a constant temperature water bath and react for 15 to 24 hours to obtain a viscous, transparent and uniform PMA-PAA solution. (3) Configuration of spinning precursor

[0044] Measure 5mL of PMA-PAA solution and add 3mL of sodium hyaluronate solution, and shake fully to obtain the spinning precursor solution.

[0045] ...

Embodiment 2

[0051] A preparation method for immobilizing enzymes in hyaluronic acid blended nanofibers, comprising the following steps:

[0052] (1) Configuration of hyaluronic acid solution

[0053] Measure 1.5mL of DMF solution and 1.5mL of distilled water into the test tube, weigh 4mg of sodium hyaluronate solid with an electronic balance, add it and dissolve to obtain a sodium hyaluronate solution.

[0054] (2) Configuration of PMA-AA binary interpolymer

[0055] Dissolve 2mL of acrylic acid and 14mL of methyl acrylate in 40mL of N,N-dimethylformamide (DMF), add 0.2-0.3g of azobisisopropionitrile, fill with nitrogen gas for 10-12min, and place at 60-80°C Heat in a constant temperature water bath and react for 15 to 24 hours to obtain a viscous, transparent and uniform PMA-PAA solution.

[0056] (3) Configuration of spinning precursor

[0057] Measure 5mL of PMA-PAA solution, add 0.5mL of sodium hyaluronate solution, and shake fully to obtain the spinning precursor solution.

[005...

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PUM

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Abstract

The invention provides a preparation method of hyaluronic acid blended nanofiber immobilized enzyme. Compared with the prior art, the method has the advantages that a hyaluronic acid-modified nanofiber with good biocompatibility and special water-retaining property is utilized by adopting an electrospinning technique; the functional nanofiber is applied to enzyme immobilization, so that the operation is relatively simple and convenient; and the blended PMA-AA@ hyaluronic acid nanofiber is steady in shape, high in porosity and large in specific surface area, and contains a lot of carboxyl. The preparation method can be applied to the field of enzyme immobilization, the supported quantity and the activity of the enzyme are significantly improved, and the method has significant functionality.

Description

technical field [0001] The invention belongs to the field of functional fibers, and in particular relates to a method for preparing enzyme-immobilized hyaluronic acid blended nanofibers. Background technique [0002] Synthesizing functional polymer materials with specific surface properties to immobilize lipase by using polymers containing functional groups as the matrix through blending technology has always been the focus of research in the field of enzyme immobilization. Although the surface of carboxyl-rich PMA-AA nanofibers has a large specific surface area, the morphology of the matrix is ​​unstable during the grafting reaction, and even completely loses the shape of nanofibers. In addition, the nanofibers exhibit hydrophobic properties. [0003] Nanofibers are prepared by electrospinning technology. On the one hand, the current enzyme immobilization carriers use ordinary polymers as the matrix. Such matrix has poor biocompatibility and it is difficult to provide a mil...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/10C12N11/08D01F8/10D01F8/18D04H1/728D01D5/00
CPCC12N9/20C12N11/08C12N11/10D01D5/0015D01F8/10D01F8/18D04H1/728
Inventor 王翠娥廖开明
Owner ANHUI UNIVERSITY OF TECHNOLOGY AND SCIENCE
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