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Method for increasing pyrroloquinoline quinone yield

A technology for pyrroloquinoline quinone and yield, which is applied in the field of increasing the yield of pyrroloquinoline quinone, can solve problems such as research on nutritional factors that rarely produce pyrroloquinoline quinone, and achieves the effects of simple method and increased yield

Inactive Publication Date: 2017-05-31
吴银娣
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The current research on microbial production of pyrroloquinoline quinone mainly focuses on the screening of high-yielding wild bacteria and the construction of genetically engineered bacteria, but there are few studies on the nutritional factors of pyrroloquinoline quinone production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Pseudomonas aureus CGMCC 1.3198 and CGMCC 1.3118 were respectively activated and then inoculated into fermentation medium for fermentation to produce PQQ. The fermentation conditions were: fermentation at 35° C. and 200 rpm for 72 hours. After the fermentation, the PQQ content in the supernatant was determined.

[0026] The fermentation medium contains: ammonium sulfate 3g / L, magnesium sulfate 0.8g / L, potassium dihydrogen phosphate 2g / L, disodium hydrogen phosphate 4g / L, manganese chloride 6mg / L, folic acid 0.25mg / L, methanol 9g / L, the content of valine is 150mg / L, the content of vitamin B2 is 8mg / L; the initial pH is 7.0.

[0027] The results showed that the PQQ contents in the fermentation supernatant of CGMCC 1.3198 and CGMCC 1.3118 were 30.96mg / L and 20.25mg / L respectively, which were higher than those obtained by fermenting the fermentation medium without adding valine and vitamin B2. 72%, 35%.

Embodiment 2

[0029] Pseudomonas aureus CGMCC 1.3198 and CGMCC 1.3118 were respectively activated and then inoculated into fermentation medium for fermentation to produce PQQ. The fermentation conditions were: fermentation at 35° C. and 200 rpm for 72 hours. After the fermentation, the PQQ content in the supernatant was determined.

[0030] The fermentation medium contains: ammonium sulfate 3g / L, magnesium sulfate 0.8g / L, potassium dihydrogen phosphate 2g / L, disodium hydrogen phosphate 4g / L, manganese chloride 6mg / L, folic acid 0.25mg / L, methanol 9g / L, the content of valine is 200mg / L, the content of vitamin B2 is 4mg / L; the initial pH is 7.0.

[0031] The results showed that the PQQ contents in the fermentation supernatants of CGMCC 1.3198 and CGMCC 1.3118 were 32.04mg / L and 19.80mg / L respectively, which were higher than those obtained by fermenting the fermentation medium without adding valine and vitamin B2. 78%, 32%.

Embodiment 3

[0033] Three strains of Pseudomonas aureus, CGMCC 1.3319, CGMCC 1.3198, and CGMCC 1.3118, were respectively activated and then inoculated into fermentation medium for fermentation to produce PQQ. The fermentation conditions were: fermentation at 35°C and 200rpm for 72h. After the fermentation, the PQQ content in the supernatant was determined.

[0034] The basic fermentation medium contains: ammonium sulfate 3g / L, magnesium sulfate 0.8g / L, potassium dihydrogen phosphate 2g / L, disodium hydrogen phosphate 4g / L, manganese chloride 6mg / L, folic acid 0.25mg / L, methanol 9g / L; initial pH 7.0.

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PUM

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Abstract

The invention discloses a method for increasing the pyrroloquinoline quinone yield, belonging to the field of biotechnology. In the invention, according to analysis of trophic factors light green yellow pseudomonas produced pyrroloquinoline quinone, the yield of pyrroloquinoline quinone can be effectively increased by controlling the content of specific amino acid and vitamin content in a medium. By adopting the method disclosed by the invention, the pyrroloquinoline quinone yield of a strain can be increased by 15-89%. Moreover, the method disclosed by the invention is simple and easy to control and applicable to industrial application.

Description

technical field [0001] The invention relates to a method for increasing the yield of pyrroloquinoline quinone, which belongs to the field of biotechnology. Background technique [0002] Pyrroloquinolinequinone (Pyrroloquinolinequinone, PQQ), chemical name is 4,5-dihydro-4,5-dioxide-1-hydropyrrole (2,3f) quinone-2,7,9-tricarboxylic acid, alias Methaxatin , was discovered by Salisbury et al. in 1979 and was originally described in bacterial cells as a redox cofactor for membrane-bound dehydrogenases. At present, PQQ is found in many different organisms, it can prevent living cells from being damaged by oxygen in vivo or in vitro; it can also be used as a nutritional factor and vitamin to promote cell growth and improve the tolerance of bacteria under extreme conditions; mammalian cells Induces production of protein kinases during differentiation; improves crop productivity by increasing insoluble phosphate availability and as a biocontrol agent; utilizes redox properties for ...

Claims

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Application Information

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IPC IPC(8): C12P17/18C12R1/38
CPCC12P17/182
Inventor 吴银娣
Owner 吴银娣
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