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Method of determining content of ondansetron hydrochloride by ultraviolet spectrophotometry

A technology of ondansetron hydrochloride and spectrophotometry, which is applied in the measurement of color/spectral characteristics, etc., can solve the problem of discomfort, rapid analysis, detection and quality control, high price of high performance liquid chromatography instruments, and high cost of liquid chromatography inspection. problems, to achieve the effect of shortened inspection time, low inspection cost and high inspection efficiency

Inactive Publication Date: 2017-05-31
TIANJIN HUAJIN PHARMA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] For the content determination of ondansetron hydrochloride, high-performance liquid chromatography is mostly used in existing standard detection methods. Although high-performance liquid chromatography has high sensitivity, good separation effect and strong specificity; but the relative price of high-performance liquid chromatography instrument Higher, the inspection cost of liquid chromatography is higher, and the operation is more time-consuming, and it is not suitable for rapid analysis detection and quality control in the production process

Method used

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  • Method of determining content of ondansetron hydrochloride by ultraviolet spectrophotometry
  • Method of determining content of ondansetron hydrochloride by ultraviolet spectrophotometry
  • Method of determining content of ondansetron hydrochloride by ultraviolet spectrophotometry

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Embodiment 1

[0012] The method for measuring ondansetron hydrochloride solution content by ultraviolet spectrophotometry may further comprise the steps:

[0013] Preparation of Ondansetron Hydrochloride Reference Substance Solution: Take 20 mg of Ondansetron Hydrochloride Reference Substance, accurately weighed, put in a 200ml volumetric flask, dissolve with 0.1mol / ml hydrochloric acid aqueous solution, and dilute with 0.1mol / ml hydrochloric acid aqueous solution To the mark, take 5ml to 50ml volumetric flask, add 0.1mol / ml hydrochloric acid aqueous solution to dilute to the mark, and make ondansetron hydrochloride 10μg / ml reference substance solution;

[0014] The preparation of sample solution to be tested: get ondansetron hydrochloride (raw material) sample 20mg to be tested, accurately weighed, put in 200ml volumetric flask, with the hydrochloric acid aqueous solution of 0.1mol / ml, and be diluted to with the hydrochloric acid aqueous solution of 0.1mol / ml For the scale, take 5ml to 50m...

Embodiment 2

[0021] The method for measuring ondansetron hydrochloride solution content by ultraviolet spectrophotometry may further comprise the steps:

[0022] Preparation of Ondansetron Hydrochloride Reference Substance Solution: Take 20 mg of Ondansetron Hydrochloride Reference Substance, accurately weighed, put in a 200ml volumetric flask, dissolve in water, and dilute to the mark with water, take 5ml into a 50ml volumetric flask, add water to dilute To the scale, make a reference substance solution of ondansetron hydrochloride 10 μg / ml;

[0023] Preparation of the sample solution to be tested: get 5 ml of the sample to be tested of ondansetron hydrochloride (injection) into a 50 ml volumetric flask, dilute to the mark with water, and prepare a sample solution to be tested containing about 10 μg / ml of ondansetron hydrochloride ;

[0024] Measure the absorbance of the reference substance solution and the sample solution to be tested at a wavelength of 310nm, and then calculate and mea...

Embodiment 3

[0030] The method for measuring the content of ondansetron hydrochloride solution by ultraviolet spectrophotometry may further comprise the steps:

[0031] Preparation of Ondansetron Hydrochloride Reference Substance Solution: Take 20 mg of Ondansetron Hydrochloride Reference Substance, accurately weighed, put in a 200ml volumetric flask, dissolve in water, and dilute to the mark with water, take 5ml into a 50ml volumetric flask, add water to dilute To the scale, make a reference substance solution of ondansetron hydrochloride 10 μg / ml;

[0032] The preparation of the sample solution to be tested: take the ondansetron hydrochloride tablet and grind it finely with a mortar, take by weighing the ondansetron hydrochloride 20mg tablet powder according to the specification, weigh it accurately, put it in a 200ml volumetric flask, add water in an appropriate amount and ultrasonically dissolve it, Dilute to the mark with water, filter the solution with filter paper or filter membrane...

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Abstract

The invention discloses a method of determining the content of ondansetron hydrochloride by ultraviolet spectrophotometry. The method comprises the following steps: taking an ondansetron hydrochloride control substance, and diluting the ondansetron hydrochloride control substance with an aqueous hydrochloric acid solution or water to produce a 10 microgram / ml control substance solution of the ondansetron hydrochloride; taking an ondansetron hydrochloride sample to be tested, and diluting the ondansetron hydrochloride sample to be tested with the aqueous hydrochloric acid solution or the water to produce a 10 microgram / ml solution of the sample, to be tested, of the ondansetron hydrochloride; putting the control substance solution and the solution of the sample to be tested at a position with wave length being 249-310nm, with the aqueous hydrochloric acid solution or the water as a blank, determining the absorbances of the control substance solution and the solution of the sample to be tested respectively, and then calculating the content of the ondansetron hydrochloride solution. According to the method, the testing time is short; the phenomenon that organic solvents including methanol, acetonitrile and isopropanol are needed to be used generally in high-performance liquid chromatography is prevented; the method is economic and environmentally friendly as the used solvent is hydrochloric acid or water; and the testing cost is relatively lower, the consumed time is short, and the detection efficiency is high.

Description

technical field [0001] The invention relates to a method for determining the content of ondansetron hydrochloride, in particular to a method for determining the content of ondansetron hydrochloride by ultraviolet spectrophotometry. Background technique [0002] For the content determination of ondansetron hydrochloride, high-performance liquid chromatography is mostly used in existing standard detection methods. Although high-performance liquid chromatography has high sensitivity, good separation effect and strong specificity; but the relative price of high-performance liquid chromatography instrument Higher, the inspection cost of liquid chromatography is higher, and the operation is more time-consuming, and it is not suitable for rapid analysis detection and quality control in the production process. Therefore, there is an urgent need for a method that is simple to operate, easy to obtain reagents, greatly shortens the test time, and effectively improves the test efficienc...

Claims

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Application Information

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IPC IPC(8): G01N21/33
CPCG01N21/33
Inventor 张奕恂梁素红赵青
Owner TIANJIN HUAJIN PHARMA
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