Detection method and test strip of furaltadone metabolites in dairy products

The technology of a furatadone and a detection method is applied to the detection method and test strip field of furatadone metabolites in dairy products, and can solve the problems of cumbersome process, long detection time, expensive detection method and the like, and achieves simple operation and detection. The effect of short time and cheap price

Inactive Publication Date: 2017-05-31
BEOSON JIANGSU FOOD SAFETY TECH CO LTD
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Problems solved by technology

[0006] Aiming at the deficiencies of the prior art, the present invention provides a method for detecting furaltadone metabolites in dairy products and a test strip, which solves the problems of high price, cumbersome process and detection time of existing detection methods for furaltadone metabolites in dairy products. long question

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  • Detection method and test strip of furaltadone metabolites in dairy products

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Embodiment Construction

[0030] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0031] Such as figure 1 As shown, the present invention provides a technical solution: a method for detecting furaltadone metabolites in dairy products, comprising the following steps:

[0032] 1. Preparation of test reagents: Measure 9 ml of concentrated hydrochloric acid with a concentration of 2 mol / L, add deionized water to 120 ml, weigh 10 grams of dipotassium hydrogen phosphate, add 400 ml of deionized water to dissolve and set the volume to 400 ml, add...

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Abstract

The invention discloses a detection method of furaltadone metabolites in dairy products. According to the detection method, reagents used for experiments are prepared via following steps: 9ml of a hydrochloric acid solution with a concentration of 2mol / L is taken via measuring, and is diluted with deionized water to 120ml; 10g of dipotassium phosphate is weighed, is dissolved in 400ml of deionized water, and an obtained solution is diluted with deionized water to 400ml so as to obtain a dipotassium phosphate sotuion; 80g of sodium chloride is added into the 400ml of dipotassium phosphate solution for complete dissolving; 3.5g of sodium hydroxide is weighed, and is dissolved with deionized water, and an obtained sodium hydroxide solution is diluted with deionized water to 100ml. The specificity of enzyme-linked immunoassay kit of furaltadone metabolites is determined based on cross reaction with corresponding substances, and the content of furaltadone in dairy products is determined based on absorbance; operation of the detection method is simple; cost is low; detection time is short; and the detection method is capable of satisfying requirements of enterprises and institutions.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a method for detecting furaltadone metabolites in dairy products and a test strip. Background technique [0002] Furaltadone, also known as furazolidone, is a nitrofuran drug. It is a synthetic antibacterial drug in the form of yellow powder or crystalline powder, with a molecular weight of 225.16 and a melting point of 245-258 degrees Celsius. The color gradually becomes darker, and the antibacterial activity of 5-nitrofuran was first discovered in the biosynthesis and microbiology experiments conducted in the United States and Germany in 1944. Subsequently, furaltadone and other homologues were listed one after another, and the drug was found to be stable. Inhibit or kill a variety of Gram-positive and negative bacteria, and have a certain effect on some protozoa and fungi. Because of its high efficacy and low price, it is widely used in medicine and animal husbandry and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78G01N21/31G01N1/28
CPCG01N21/78G01N1/28G01N21/3151
Inventor 周合张根义张进周朱晨杨敏胡彬吴念绮
Owner BEOSON JIANGSU FOOD SAFETY TECH CO LTD
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