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Content determination method of Xuebijing intermediate multi-index components

A measurement method and intermediate technology, applied in the field of medicine, can solve the problems of synchronous determination of functional components, long manual occupation time, and long detection cycle, so as to shorten the inspection cycle, save the consumption of organic reagents, and improve work efficiency. Effect

Active Publication Date: 2017-05-31
TIANJIN CHASE SUN PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The intermediate of Xuebijing contains three functional components—hydroxysafflor yellow A, paeoniflorin, and ferulic acid. Currently, the three components are qualitatively and quantitatively detected by three different liquid chromatography conditions, and the determination of the content of the functional components cannot be carried out. Simultaneously, the cost of reagents and consumables is large, the labor time is long, and the detection cycle is long

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Examples

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Embodiment 1

[0094] The method for detecting the content of the functional components of the Xuebijing intermediate of the present invention comprises the following steps:

[0095] (1) Liquid chromatography conditions:

[0096] Determination according to high-performance liquid-liquid chromatography:

[0097] Chromatographic column: octadecylsilane bonded silica gel;

[0098] Mobile phase: methanol: acetic acid aqueous solution (add 5 ml of 36% glacial acetic acid aqueous solution to 1000 ml of water) (20:80);

[0099] Flow rate: 1.0ml / min;

[0100] Column temperature: 30°C;

[0101] Detection wavelength: 230nm;

[0102] Injection volume: 20μl;

[0103] Running time: 60min,

[0104] (2) Determination of relative correction factor with paeoniflorin as internal reference:

[0105] a. Preparation of standard curve solution:

[0106] Preparation of hydroxysafflower yellow A standard curve solution: take about 20 mg of hydroxysafflower yellow A reference substance, weigh it accurately, ...

Embodiment 2

[0145] The method for detecting the content of the functional components of the Xuebijing intermediate of the present invention comprises the following steps:

[0146] (1) Liquid chromatography conditions:

[0147] Determination according to high-performance liquid-liquid chromatography:

[0148] Chromatographic column: octadecylsilane bonded silica gel;

[0149] Mobile phase: Methanol: Acetic acid aqueous solution (add 5ml of 36% glacial acetic acid aqueous solution to 1000ml water) (10:90);

[0150] Flow rate: 0.8ml / min;

[0151] Column temperature: 30°C;

[0152] Detection wavelength: 230nm;

[0153] Injection volume: 20μl;

[0154] Running time: 60min,

[0155] (2) Determination of relative correction factor with paeoniflorin as internal reference:

[0156] a. Preparation of standard curve solution:

[0157] Preparation of hydroxysafflower yellow A standard curve solution: take about 20 mg of hydroxysafflower yellow A reference substance, weigh it accurately, put i...

Embodiment 3

[0177] The method for detecting the content of the functional components of the Xuebijing intermediate of the present invention comprises the following steps:

[0178] (1) Liquid chromatography conditions:

[0179] Determination according to high-performance liquid-liquid chromatography:

[0180] Chromatographic column: octadecylsilane bonded silica gel;

[0181] Mobile phase: Methanol: Acetic acid aqueous solution (add 5ml of 36% glacial acetic acid aqueous solution to 1000ml water) (30:70);

[0182] Flow rate: 1.2ml / min;

[0183] Column temperature: 30°C;

[0184] Detection wavelength: 230nm;

[0185] Injection volume: 20μl;

[0186] Running time: 60min,

[0187] (2) Determination of relative correction factor with paeoniflorin as internal reference:

[0188] a. Preparation of standard curve solution:

[0189] Preparation of hydroxysafflower yellow A standard curve solution: take about 20 mg of hydroxysafflower yellow A reference substance, weigh it accurately, put i...

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Abstract

The invention relates to a content determination method of Xuebijing intermediate multi-index components. The content determination method has the advantages that the method is good in precision, good in reproducibility, good in stability and capable of being used to detect the content of hydroxyl carthamin yellow A, paeoniflorin, ferulic acid and benzoic acid in Xuebijing injection intermediate, product quality can be increased favorably, and the life quality of people can be increased.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for detecting and analyzing the contents of hydroxysafflor yellow A, paeoniflorin, ferulic acid and benzoic acid in Xuebijing intermediates. Background technique [0002] Xuebijing Injection is a brownish-yellow to brownish-red clear liquid, the main ingredients are safflower, Chuanxiong, Danshen and angelica, etc., the main function is to remove blood stasis and detoxify, and it is used for warm-heat diseases such as fever, shortness of breath, palpitations, and restlessness. Syndrome of blood stasis and poison due to dryness. With the deepening of clinical research, its scope of application is also expanding, and can be used for kidney, lung, cardiovascular, sepsis, disseminated intravascular coagulation (DIC), multiple organ dysfunction syndrome (MDOS), Severe acute pancreatitis, diabetic ketoacidosis (DKA) and other diseases. [0003] Xuebijing Injecti...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 胡小锋童艳刘方石秀英姜艳春
Owner TIANJIN CHASE SUN PHARM CO LTD
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