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Mobile phase formulation for measuring carotenoid through liquid chromatography and application of mobile phase formulation

A technology of carotene and liquid chromatography, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of poor peak shape, inseparable peaks, low response value, etc., and achieve good linear relationship, good peak shape, The effect of high spike recovery

Active Publication Date: 2017-05-31
AGRO BIOLOGICAL GENE RES CENT GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the use of C30 column liquid chromatography is mostly a method for the separation of a single or a few carotenoids. In the simultaneous determination of five or more methods, it often occurs that the peak shape is not good, the peaks cannot be separated, and the relative deviation Large, low response value and other issues

Method used

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  • Mobile phase formulation for measuring carotenoid through liquid chromatography and application of mobile phase formulation
  • Mobile phase formulation for measuring carotenoid through liquid chromatography and application of mobile phase formulation
  • Mobile phase formulation for measuring carotenoid through liquid chromatography and application of mobile phase formulation

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Embodiment 1

[0041] 1. Reagents and Instruments

[0042] 1.1 Reagents

[0043] Standard products: Lutein, Zeaxanthin, α-Cryptoxanthin, β-Cryptoxanthin, α-carotene, β -Carotene (β-carotene), γ-carotene (γ-carotene), (6R)-δ-carotene ((6R)-δ-carotene), ε-carotene (ε-carotene), lycopene (Lycopene) was purchased from Sigma; Methanol, ethanol and tert-butyl methyl ether (MTBE) were pure reagents for chromatography; ammonium acetate and 2,6-di-tert-butyl-4-methylphenol (BHT) were pure reagents for mass spectrometry ; The experimental water was Milli-Q ultrapure water.

[0044] 1.2 Instruments

[0045] American DIONEX UltiMate 3000 high performance liquid chromatography workstation, equipped with HPG-3200RS binary gradient pump, TCC-3000RS column thermostat, WPS-3000TFC autosampler, DAD-3000RS diode array detector and Chromelein6.8 analysis software; CPA224S type One-ten-thousandth balance, Sartorius, Germany; SB5200DTS ultrasonic cleaning machine, Ningbo Xinzhi.

[0046] 1.3 Standard solutio...

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Abstract

The invention discloses a mobile phase formulation for measuring carotenoid through liquid chromatography and application of the mobile phase formulation and belongs to the field of methods for separating and metabolizing micromolecules through liquid chromatography. The mobile phase formulation comprises, by volume, A1 phase: 90-100% of methanol-water and B1 phase: 90-100% of tert-butyl methyl ether-methanol. By the mobile phase formulation, chromatograms and testing results of 7-10 carotenoids which are independent in components, good in peak pattern and linear relation, high in adding standard recovery rate and little in relative error can be acquired simply, accurately and quickly (the whole process can be completed within 40min).

Description

technical field [0001] The invention belongs to the field of methods for separating metabolic small molecules by liquid chromatography, and in particular relates to a mobile phase formula for measuring carotenoids by liquid chromatography and its application. Background technique [0002] As a special metabolite of small molecules, carotenoids can effectively scavenge free radicals, have significant anti-oxidation, improve human immunity, delay aging, prevent cancer and fight cancer, and play an active and important role in protecting human health. Since the human body cannot synthesize such substances by itself, supplementing artificially synthesized substances of this kind has no effect on disease prevention and anti-cancer, so intake from natural food has become the only inevitable way to obtain them. The carotenoids distributed in food mainly include lutein, zeaxanthin, α-cryptoxanthin, β-cryptoxanthin, α-carotene, β-carotene, γ-carotene, (6R)-δ- Carotene, ε-carotene, e...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/34
CPCG01N30/34
Inventor 孔谦晏石娟陈庄张群洁黄文洁胡建广
Owner AGRO BIOLOGICAL GENE RES CENT GUANGDONG ACADEMY OF AGRI SCI
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