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Helicobacter pylori IgG antibody enzyme linked immunosorbent assay (ELISA) diagnostic kit

A technology for Helicobacter pylori and immunodiagnosis, which is applied in the field of medical biology, can solve the problems of poor stability and short storage period, and achieve the effects of high accuracy, improved storage period and good stability

Active Publication Date: 2017-06-06
UNIMED BIOTECH SHANGHAI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the ubiquitous problems of short storage period and poor stability in the existing Helicobacter pylori antibody ELISA kits, the present invention provides a Helicobacter pylori IgG antibody ELISA kit, which has good Excellent stability, valid for 1 year at room temperature and up to 3 years at 4°C, significantly improving the shelf life of the kit

Method used

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  • Helicobacter pylori IgG antibody enzyme linked immunosorbent assay (ELISA) diagnostic kit

Examples

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Effect test

Embodiment 1

[0025] Embodiment 1, Helicobacter pylori IgG antibody ELISA diagnostic kit of the present invention

[0026] The kit of this embodiment consists of Helicobacter pylori antigen-coated plate (96 wells), 1 bottle of sample diluent, 1 bottle of enzyme labeling solution, 1 bottle of washing solution, 1 bottle of chromogenic solution, 1 bottle of negative control substance, and 1 bottle of positive control substance 1 bottle and 1 bottle of stop solution.

[0027] Wherein, the preparation process of the Helicobacter pylori antigen-coated plate is as follows: after the Helicobacter pylori antigen for coating is diluted with 0.05mol / L carbonate buffer solution of pH 9.5, the plate is coated with 0.1mL / well. After absorbing at 4°C for 24 hours, wash the plate with 0.05mol / L pH9.5 phosphate buffer, then block with blocking solution at 4°C for 18 hours, discard the excess blocking solution, and vacuum After drying, the Helicobacter pylori antigen-coated plate was prepared.

[0028] The...

Embodiment 2

[0036] Embodiment 2, Helicobacter pylori IgG antibody ELISA diagnostic kit of the present invention

[0037] The kit of this embodiment consists of Helicobacter pylori antigen-coated plate (96 wells), 1 bottle of sample diluent, 1 bottle of enzyme labeling solution, 1 bottle of washing solution, 1 bottle of chromogenic solution, 1 bottle of negative control substance, and 1 bottle of positive control substance 1 bottle and 1 bottle of stop solution.

[0038]Wherein, the preparation process of the Helicobacter pylori antigen-coated plate is as follows: after the Helicobacter pylori antigen for coating is diluted with 0.05mol / L carbonate buffer solution of pH 9.5, the plate is coated with 0.1mL / well. After absorbing in the microwell plate at 8°C for 26 hours, wash the plate with 0.05mol / L pH9.5 phosphate buffer, then block with blocking solution at 8°C for 20 hours, discard the excess blocking solution, and vacuum After drying, the Helicobacter pylori antigen-coated plate was p...

Embodiment 3

[0047] Embodiment 3, Helicobacter pylori IgG antibody ELISA diagnostic kit of the present invention

[0048] The kit of this embodiment consists of Helicobacter pylori antigen-coated plate (96 wells), 1 bottle of sample diluent, 1 bottle of enzyme labeling solution, 1 bottle of washing solution, 1 bottle of chromogenic solution, 1 bottle of negative control substance, and 1 bottle of positive control substance 1 bottle and 1 bottle of stop solution.

[0049] Wherein, the preparation process of the Helicobacter pylori antigen-coated plate is as follows: after the Helicobacter pylori antigen for coating is diluted with 0.05mol / L carbonate buffer solution of pH 9.5, the plate is coated with 0.1mL / well. After absorbing in the microwell plate at 6°C for 24 hours, wash the plate with 0.05mol / L pH9.5 phosphate buffer, then block with blocking solution at 6°C for 18 hours, discard the excess blocking solution, and vacuum After drying, the Helicobacter pylori antigen-coated plate was ...

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Abstract

The invention belongs to the technical field of medical biology, and in particular relates to a helicobacter pylori IgG antibody enzyme linked immunosorbent assay (ELISA) diagnostic kit. The kit consists of a helicobacter pylori antigen-coated plate, sample diluent, enzyme-labeled liquid, a cleaning solution, a developing solution, a negative control substance, a positive control substance and stop buffer. The kit is high in sensitivity, strong in specificity and high in accuracy, and has good stability; the shelf life of the kit is one year under the condition of room temperature, and the shelf life of the kit reaches three years under the condition of 4 DEG C, so that the shelf life of the kit is obviously prolonged.

Description

technical field [0001] The invention belongs to the technical field of medicine and biology, and in particular relates to an ELISA diagnostic kit for Helicobacter pylori IgG antibody. Background technique [0002] Helicobacter pylori (Hp) is a Gram-negative helical bacterium that colonizes and reproduces in gastric epithelial cells. Helicobacter pylori infection is not only the main pathogen of chronic gastritis and peptic ulcer, but also a risk factor for gastric cancer, and it is also related to many diseases outside the stomach. Therefore, the diagnosis of Helicobacter pylori infection has increasingly attracted the attention of many countries and regions. There are many methods for laboratory diagnosis and detection of Helicobacter pylori infection, including traditional direct microscopic examination of pathological specimens, bacterial culture of living tissue and urease test, etc., but these methods are all invasive and traumatic examinations, which cause great pain ...

Claims

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Application Information

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IPC IPC(8): G01N33/569
CPCG01N33/56911
Inventor 陈立国邹伟权张亚丽李庆祥母润红王涛苏焱
Owner UNIMED BIOTECH SHANGHAI CO LTD
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