Cryopreserved recovery reagent for pet testicular tissue and cryopreserved recovery method

A reagent and cryopreservation technology, applied in biochemical equipment and methods, tissue culture, preservation of human or animal bodies, etc., can solve problems that have not been developed and applied, and achieve high tissue recovery activity, convenient use, and long shelf life Effect

Inactive Publication Date: 2017-06-13
上海尚维生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This type of cryopreservation technology has not been developed and applied in the field of pet health

Method used

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  • Cryopreserved recovery reagent for pet testicular tissue and cryopreserved recovery method
  • Cryopreserved recovery reagent for pet testicular tissue and cryopreserved recovery method
  • Cryopreserved recovery reagent for pet testicular tissue and cryopreserved recovery method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-5

[0057] Embodiment 1-5: Preparation of pet testicular tissue cryopreservation reagent

[0058] (1) Taking the preparation of 100ml cryopreservation reagent 1 as an example: Measure dimethyl sulfoxide (purchased from SIGMA), propylene glycol (purchased from SIGMA), and MEM medium (purchased from GIBCO) according to the amounts shown in Table 1 Put it into a 250ml blue-mouth bottle (purchased from Shu Niu), shake gently to mix; weigh Lycium barbarum polysaccharide (purchased from Sigma), cholesterol (purchased from SIGMA), polyvinylpyrrolidone ((PVP) purchased from SIGMA) 1. Glucose (purchased from SIGMA) was added to the above bottle, shaken and mixed. After the solids are completely dissolved, use a 0.22 μm filter (purchased from MILLIPORE) to filter and sterilize, filter into a 100ml blue-necked bottle (purchased from Shuniu) that has been sterilized by autoclaving, and store at 4°C for later use;

[0059] (2) Take the preparation of 100ml cryopreservation reagent 2 as an exa...

Embodiment 6-10

[0065] Embodiment 6-10: Preparation of Pet Testicular Tissue Recovery Reagent

[0066] (1) Take the preparation of 100ml resuscitation reagent 1 as an example: measure vitamin E (oily liquid, purity ≥98%) (purchased from SIGMA) and MEM medium (purchased from GIBCO) according to the amounts shown in Table 2. into a 250ml blue-mouth bottle (purchased from Shuniu), shake gently and mix well; weigh Lycium barbarum polysaccharide (purchased from Snotte Biology), cholesterol (purchased from SIGMA), polyvinylpyrrolidone (purchased from SIGMA), glucose (purchased from SIGMA), glucose (purchased from SIGMA) into the above bottle, shake to mix. After the solids are completely dissolved, use a 0.22 μm filter (purchased from MILLIPORE) to filter and sterilize, filter into a 100ml blue-necked bottle (purchased from Shuniu) that has been sterilized by autoclaving, and store at 4°C for later use;

[0067] (2) Taking the preparation of 100ml resuscitation reagent 2 as an example: Measure vit...

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PUM

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Abstract

The invention provides a cryopreserved recovery reagent set for a pet testicular tissue. The cryopreserved recovery reagent set comprises (1) a cryopreserved reagent 1: 2-7W / V% of lycium barbarum polysaccharide, 0.5-5W / V% of cholesterol, 6-14V / V% of dimethyl sulfoxide, 2-7V / V% of propylene glycol and 80-90V / V% of MEM culture medium; (2) a cryopreserved reagent 2: 8-10W / V% of lycium barbarum polysaccharide, 0.5-5W / V% of cholesterol, 6-20V / V% of dimethyl sulfoxide, 5-15V / V% of propylene glycol and 70-80V / V% of MEM culture medium; (3) a cryopreserved reagent 3: 15-20W / V% of lycium barbarum polysaccharide, 0.5-5W / V% of cholesterol, 6-20V / V% of dimethyl sulfoxide, 5-15V / V% of propylene glycol and 70-80V / V% of MEM culture medium. The invention also provides a recovery reagent set for the pet testicular tissue and a cryopreserved recovery method for the pet testicular tissue.

Description

technical field [0001] The invention relates to cryopreservation of testicular tissue, in more detail, the present invention relates to pet testicular tissue cryopreservation and recovery reagent and pet testicular tissue cryopreservation and recovery method. Background technique [0002] Pets (mainly dogs and cats as companion animals) can accompany humans, relieve the pressure in daily life, delight the mood, promote human health, and become a member of the family. With the development of my country's economy and the aggravation of aging, the demand for pets is increasing. While the number of pets has increased, industries such as pet food, pet supplies, and pet training have developed vigorously. The field of pet health has not received enough attention and has not yet been standardized. Compared with the human health market, the pet health market has a lot of room to be tapped. Caring for pets and paying attention to pet health. [0003] At home and abroad, it is gen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02C12N5/071
CPCA01N1/0221A01N1/0284C12N5/0683C12N2500/34C12N2500/36C12N2500/38C12N2500/50C12N2501/90
Inventor 朱梅花
Owner 上海尚维生物科技有限公司
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