Method for preparing small-molecule low peptides of donkey-hide gelatin

Abstract

The invention discloses a method for preparing small-molecule low peptides of donkey-hide gelatin. The method includes the steps of 1), dissolving the donkey-hide gelatin and configuring the same to a donkey-hide gelatin water solution with set concentration; 2), performing high-pressure hydrolysis by adding acid, namely mixing the donkey-hide gelatin water solution in the step 1) with oxalic acid, adjusting the pH value to 1.5-2.5, and performing high-pressure hydrolysis; 3), pH call back, namely adjusting the pH value of a product after high-pressure hydrolysis to 3.5-4.5; 4), decolorization and removal, namely adding activated carbon for decolorization and removal and then removing the activated carbon to obtain the small-molecule low peptides of the donkey-hide gelatin. An acid environment is made by adopting the organic acid, the oxalic acid is of the weak acid, and peptide bonds among proteins of the donkey-hide gelatin can be fully broken and dissolved into the small-molecule low peptides; the proteins are degenerated rapidly by adopting a high-pressure cooking method to have the peptides bonds broken; the two methods are combined in the method, so that the small-molecule low peptides with small and even molecule weight are favorably obtained, and the small-molecule-weight low peptides can be well absorbed and utilized by the human body.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a method for preparing donkey-hide gelatin small-molecule low-peptide. Background technique [0002] Amino acid is the basic unit of protein. The covalent bond formed by the dehydration of the carboxyl group of one amino acid and the amino group of another amino acid is called a peptide bond. The biologically active molecule formed by connecting two or more amino acids through a peptide bond is called a peptide. Peptide chains are modified by folding to form proteins. Among them, oligopeptides consisting of 2 to 4 amino acids are called small molecule low peptides or small peptides. [0003] Studies have shown that the protein content in donkey-hide gelatin is about 60-80%, mainly collagen and its degraded polypeptide substances. Donkey-hide gelatin is of great benefit to human health. However, the human body cannot directly absorb large molecular proteins. It need...

AI Technical Summary

Problems solved by technology

[0004] Aiming at the deficiencies in the prior art, the present invention provides a method for preparing donkey-hide gelatin small-molecule low-peptide. The method uses organic acids to make an acidic environment, and uses high-pressure cooking to hydrolyze donkey-hide gelatin raw materials, which solves the problem of difficult hydrolysis of donkey-hide gelatin collagen, enzymolysis The molecular weight of the product is not concentrated, etc.

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