Low-cost medium composition for propagation of soybean meal fermentation strains in cold regions

A fermented strain, low-cost technology, applied in the direction of microorganism-based methods, bacteria, microorganisms, etc., can solve the problem of high cost

Active Publication Date: 2021-07-06
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Glucose, peptone and yeast extract are used as the main carbon and nitrogen sources of the liquid culture medium, and the cost is relatively high

Method used

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  • Low-cost medium composition for propagation of soybean meal fermentation strains in cold regions
  • Low-cost medium composition for propagation of soybean meal fermentation strains in cold regions
  • Low-cost medium composition for propagation of soybean meal fermentation strains in cold regions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: 2% soybean meal was used as nitrogen source, and 1% corn + 1% wheat bran, 1.5% corn + 0.5% wheat bran, 0.5% corn + 1.5% wheat bran were used as carbon source to test, each treatment group Inorganic salt content is the same. The amount of fermentation in a 500ml Erlenmeyer flask was 250ml, placed under the condition of 37°C for 48 hours, and the number of live bacteria was determined. 3 replicates per treatment. The test results are shown in Table 1.

[0017] Table 1 Effects of different carbon source medium components on the number of bacteria (×10 10 cfu / mL)

[0018]

[0019] It can be seen from Table 1 that under the same conditions of nitrogen source and inorganic salt, 1.5% corn + 0.5% wheat had the highest number of live bacteria, significantly higher than the combination of 0.5% corn + 1.5% wheat bran, and higher than 1% corn + 1% wheat bran. Choose 1.5% corn + 0.5% wheat as carbon source.

Embodiment 2

[0020] Example 2: 1.5% corn+0.5% wheat was used as carbon source, and 1.0% soybean meal, 1.5% soybean meal and 2.0% soybean meal were used as nitrogen source to carry out experiments. The test design and test conditions are the same as in Example 1.

[0021] Table 2 Effects of different nitrogen sources on the number of bacteria (×10 10 cfu / mL)

[0022]

[0023] It can be seen from Table 2 that there is no significant difference in the number of viable Bacillus subtilis cultured with 1.0% soybean meal, 1.5% soybean meal and 2.0% soybean meal. Under the condition of 1.5% corn + 0.5% wheat bran as carbon source, choosing 1.0% soybean meal as nitrogen source did not affect the growth and reproduction of microorganisms.

Embodiment 3

[0024] Example 3: Using different medium formulations (see Table 3), strains were multiplied at 30°C, and fermented in a 5L narrow-mouth bottle with a fermentation volume of 3L, and each treatment was replicated three times. Using the number of viable bacteria in the fermentation broth as an index, the low-cost medium formula for the expansion of soybean meal fermentation strains was screened.

[0025] Table 3 Optimization of Bacillus subtilis liquid fermentation medium composition

[0026]

[0027]

[0028] The number of Bacillus subtilis (see Table 4) obtained after fermentation of different medium formulas showed certain differences, among which the number of viable bacteria cultured at 37°C was the highest, reaching 2.23×10 10 cfu / mL; formula 2 had the highest number of viable bacteria cultured at 30°C, reaching 2.08×10 10 cfu / mL, slightly lower than the control group, but higher than the other 4 formulations, the difference was significant (P<0.05). At 30°C, the e...

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Abstract

The invention provides a low-cost culture medium composition for propagation of soybean meal fermentation strains in cold regions, and belongs to the technical field of propagation medium for soybean meal fermentation strains. The present invention consists of 1.3-1.7% of corn, 0.3-0.7% of wheat bran, 0.8-1.2% of soybean meal, 0.1-0.5% of calcium carbonate, 0.0005-0.004% of manganese sulfate, 0.04-0.3% of sodium chloride, dihydrogen phosphate It consists of 0.05-0.4% of potassium, 0.005-0.04% of magnesium sulfate and the rest of water. The invention further pursues the combination effect of raw materials: 1.5% corn and 0.5% wheat bran+1.0% soybean meal are used, and the overall carbon-nitrogen ratio is closer to the needs of microorganisms. The crude protein content in the formula is 20.66%, the nitrogen content is 3.3%, and the carbon-nitrogen ratio is about 12:1. Wheat bran is rich in vitamins and some unknown growth factors, which can obviously promote the growth and reproduction of microorganisms and animals. The nutrition provided by the combination of corn, wheat bran and soybean meal is closer to the needs of microorganisms.

Description

technical field [0001] The invention relates to a low-cost culture medium composition for propagation of soybean meal fermentation strains in cold regions, and belongs to the technical field of propagation medium for soybean meal fermentation strains. Background technique [0002] The nutritional value of soybean meal is greatly improved after fermentation, and it can replace 50% to 80% of fish meal in piglet and chick feed, which can reduce feed cost. Fermented soybean meal is a conventional feed, and the demand is large, and the production output of enterprises is also large. The traditional strain propagation medium uses glucose as the main carbon source, and peptone and yeast extract as the main nitrogen source. In the liquid medium, generally the addition amount of glucose is 2% to 3%, and the addition amount of peptone and yeast extract is about 0.5% and 0.5%, and the price of these two substances is about 35 yuan to 50 yuan / kg. The price is 3.5 yuan / kg. For every t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/125
CPCC12N1/20
Inventor 李杰陈雷齐瑛琳张刘运
Owner NORTHEAST AGRICULTURAL UNIVERSITY
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