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Application of bacillus methylotrophicus BH21 as well as microbial fertilizer of bacillus methylotrophicus BH21 and preparation method for microbial fertilizer

A methylotrophic, microbial fertilizer technology is applied in the preparation of organic fertilizers, botanical equipment and methods, chemicals used for biological control, etc. Nutrient absorption, good nutrition, the effect of increasing plant yield

Active Publication Date: 2017-06-20
BIOLOGY INST OF HEBEI ACAD OF SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, at present, there are few studies on methylotrophic bacillus as a salt-tolerant bacterium to improve salinized soil in facilities and promote plant growth, and there is no research on methylotrophic bacillus that can promote plant growth and rooting.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1 The rooting effect of methylotrophic Bacillus BH21 on isolated cucumber leaves

[0014] Pick healthy leaves of similar size from healthy cucumber plants, prepare methylotrophic Bacillus BH21 bacterial solutions diluted 4 times, 5 times and 10 times with distilled water, soak the cucumber leaves in the diluted solution for 5 minutes, and let them dry in the air. Dried cucumber leaves soaked in distilled water as a control. The treated cucumber leaves were placed in a petri dish lined with moist gauze and a proper amount of distilled water, and incubated at 22 °C to observe the growth status of the leaves.

[0015] After moisturizing for 4 days, the base of the petiole of the cucumber leaves treated with the fermentation broth of methylotrophic Bacillus BH21 all appeared rooting phenomenon, among which the rooting effect was most obvious with the fermentation broth diluted 4 times, the average number of roots was 12, and the root length was 21.5cm The average n...

Embodiment 2

[0016] Example 2 The rooting effect of methylotrophic Bacillus BH21 on isolated tomato leaves

[0017] Pick healthy leaves of similar size from healthy tomato plants, prepare methylotrophic Bacillus BH21 bacterial solutions diluted 5 times and 10 times with distilled water, soak tomato leaves in the diluted solution for 5 min, and then dry them; Dried tomato leaves were soaked in distilled water as a control. The tomato leaves were placed in a petri dish lined with moist gauze and appropriate amount of distilled water, and incubated at 22°C to observe the growth status of the leaves.

[0018] After moisturizing for 4 days, the leaves of tomato leaves treated with the 5-fold and 10-fold diluted fermentation broth of methylotrophic Bacillus BH21 were bright green and flat, and no rooting phenomenon appeared in all treatments. Observing the base of the tomato petiole after moisturizing for 7 days, it was found that the tomato leaves treated with the 5-fold dilution and the 10-fo...

Embodiment 3

[0019] Example 3 Effect of Methylotrophic Bacillus BH21 on Salt Tolerance of Plants

[0020] Cucumber seeds from Jinlv Small Vegetable Garden were raised in the greenhouse until the seedlings reached the stage of 1 leaf and 1 heart by using the hole tray seedling method, and the roots of the seedlings were cleaned and moved into a hydroponic box (the box was 20cm long, 15cm wide, and 10cm high). A foam board with a thickness of 1 cm is used and a circular hole with a diameter of 0.5 cm is drilled on it, and cucumber seedlings are planted on the foam board, and 9 cucumbers are planted in each hydroponic box, arranged evenly. Fill the hydroponic box with MM nutrient solution, and treat the cucumber with salt and bacteria when it grows to two leaves and one heart. Four treatments were set up in the experiment. Treatment 1 was MM nutrient solution containing NaCl with a concentration of 100mmol / L; 6 cfu / mL of BH21; treatment 3 was MM nutrient solution. Cucumbers were cultivated ...

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Abstract

The invention discloses an application of bacillus methylotrophicus BH21 with a preservation number of CGMCC No.11840 in preparing a microbial fertilizer with effects of promoting growth and improving salt resistance of plants, and discloses the microbial fertilizer which comprises excrements of poultry and livestocks, mushroom residues, breeding padding and the bacillus methylotrophicus BH21. A preparation method for the microbial fertilizer comprises the following steps: uniformly mixing the bacillus methylotrophicus BH21 and thoroughly decomposed excrements, performing fermentation for 4-5 days continuously by adopting a strip type stacking fermenting mode, turning over every day in a fermentation process, controlling a pile temperature not to exceed 45 DEG C, and obtaining the microbial fertilizer after fermentation is completed. The bacterial strain has relatively strong salt resistance, can promote plants to take root and grow, and can be combined with the thoroughly decomposed organic fertilizer to obtain the bio-organic fertilizer to provide rich nutrients for plant growth; and nutrients of the organic fertilizer also provide good nutrients for survival and colonization of the multifunctional bacterial strain.

Description

technical field [0001] The invention relates to the technical field of application of microbial strains, in particular to the application of a methylotrophic bacillus BH21, a microbial fertilizer prepared therefrom and a preparation method thereof. Background technique [0002] In recent years, facility planting in my country has developed rapidly. In 2015, the cultivated area of ​​protected agriculture in my country was 4.109 million hectares, accounting for about 85% of the total area of ​​protected agriculture in the world. However, with the rapid development of protected cultivation, long-term surface covering cultivation has greatly changed the physical and chemical properties and biological characteristics of the soil, especially the surface accumulation of soil salinity is obvious, and the problem of secondary salinization of soil is common. The soil with secondary salinization not only inhibits the growth and development of cultivated crops, but also causes a declin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01P21/00C05G3/00C05F17/00
CPCA01N63/00C05F3/00C05F17/00C05G3/00C05F11/00C05F11/08Y02W30/40
Inventor 黄亚丽魏新燕黄媛媛贾振华叶婧赵芊宋聪朱昌雄
Owner BIOLOGY INST OF HEBEI ACAD OF SCI
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