A clean production method of Zhongshengmycin master drug
A technology of clean production and bacteriocin, applied in the direction of microorganism-based methods, biochemical equipment and methods, chemical instruments and methods, etc., can solve the problem of increasing the pressure of biochemical treatment in the company's sewage treatment station and the loss of effective components of bacteriocin Waste, increasing energy consumption and cost of sewage treatment, etc., to achieve the effect of improving environmental compatibility, reducing the use of water resources, and good economic and social benefits
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Embodiment 1
[0029] 1. Take the tank washing water of the fermentation tank in production, the washing water of the spray tower, the water foam dust removal wastewater in the spraying process, the cleaning water of the ceramic membrane system, the cleaning water of the membrane system of the nanofiltration membrane system, and the permeate filtered out of the nanofiltration membrane system. As the preparation water of Zhongshengmycin shake flask fermentation medium, the preparation water of shake flask fermentation medium COD and NH 3 -N and other contents are as follows:
[0030]
[0031] 2. Use uncontaminated drinking water to prepare fermentation medium as a control, and carry out shake flask fermentation verification simultaneously.
[0032] 3. After the preparation of each group of medium, use 20% sulfuric acid or 20% sodium hydroxide to adjust the pH to 7.3-7.5, and divide them into triangular shaker flasks by a well-known method in the industry, with 6 bottles in each group, and ...
Embodiment 2
[0036] Use the fermenter tank to put tank washing water (Zhongshengmycin content 1.25g / L, COD content 11820mg / L, NH 3 -N content 51.56mg / L) to prepare another batch of Zhongshengmycin fermenter fermentation medium, adjust the pH to 7.3-7.5, and sterilize and inoculate the prepared medium in the fermenter with known methods in the industry , cultivating, feeding, and putting in tanks, and measuring the content of bacteriocin in the fermentation liquid of putting in tanks is 11.12g / L. Zhongshengmycin is placed in a tank and fermented through a ceramic membrane system for liquid-solid separation → the filtrate is concentrated by a nanofiltration membrane system → the concentrated solution is mixed with the solids separated by the ceramic membrane system in proportion and then spray-dried to obtain Zhongshengmycin parent The quality indicators of the finished medicine and Zhongshengmycin master drug are as follows:
[0037]
Embodiment 3
[0039] Wash tower water with spray tower (zhongshengmycin content 2.38g / L, COD content 48483mg / L, NH 3-N content 155.21mg / L) Prepare another batch of Zhongshengmycin fermentation medium, adjust the pH to 7.3-7.5, and carry out disinfection, inoculation and cultivation of the culture medium in the prepared fermentation tank by the methods known in the industry , feed, put the tank, and measure the content of bacteriocin in the fermentation liquid of the tank to be 10.58g / L. Zhongshengmycin is placed in a tank and fermented through a ceramic membrane system for liquid-solid separation → the filtrate is concentrated by a nanofiltration membrane system → the concentrated solution is mixed with the solids separated by the ceramic membrane system in proportion and then spray-dried to obtain Zhongshengmycin parent The quality indicators of the finished medicine and Zhongshengmycin master drug are as follows:
[0040]
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