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Overcompensation cultural method improving content of proteins of heterotrophic microalgae

A technology of protein content and culture method, which is applied in the cultivation of chlorella and increases the protein production of chlorella, can solve the problem of low protein content of heterotrophic chlorella, and achieves favorable conditions for growth and protein accumulation and mild conditions , The effect of simple process

Inactive Publication Date: 2017-06-20
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to provide a method for increasing the protein content in chlorella organisms by utilizing the overcompensation absorption effect for the defect that the protein content of heterotrophic chlorella is not high at present

Method used

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  • Overcompensation cultural method improving content of proteins of heterotrophic microalgae
  • Overcompensation cultural method improving content of proteins of heterotrophic microalgae
  • Overcompensation cultural method improving content of proteins of heterotrophic microalgae

Examples

Experimental program
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Effect test

Embodiment 1

[0039] the solid BG11 + Chlorella on plate medium was inoculated into sterilized BG11 + In the liquid culture medium, 25 ℃, 180rpm, culture 3 days, serve as primary seed liquid.

[0040] Inoculate the sterilized BG11 with a 10% (V / V) inoculum of the primary seed liquid + In liquid culture medium, cultured for 3 days, as the secondary seed solution. The culture conditions are the same as the seed solution.

[0041] The secondary seed solution was inoculated with 10% (V / V) inoculum in sterilized low nitrogen concentration of 3 mmol L -1 BG11 + medium. The culture conditions were as follows: temperature 25°C, shaker speed 180 rpm.

[0042] When Chlorella grows to the end of the exponential phase, that is, on the 3rd day of the culture process, the algae cells are collected by centrifugation at 4000rpm for 8 min under aseptic conditions.

[0043] Immediately resuspend the algal cells in an equal volume of fresh sterilized 18 mmol L -1 Continue culturing at 25°C in high nit...

Embodiment 2

[0047] the solid BG11 + Chlorella on plate medium was inoculated into sterilized BG11 + In the liquid culture medium, 25 ℃, 180rpm, culture 3 days, serve as primary seed liquid.

[0048] Inoculate the sterilized BG11 with a 10% (V / V) inoculum of the primary seed liquid + In liquid culture medium, cultured for 3 days, as the secondary seed solution. The culture conditions are the same as the seed solution.

[0049] The secondary seed solution was inoculated with 10% (V / V) inoculum in sterilized low nitrogen concentration of 3mmol L -1 BG11 + medium. The culture conditions were as follows: temperature 25°C, shaker speed 180 rpm.

[0050] When Chlorella grows to the end of the exponential phase, that is, on the 3rd day of the culture process, the algae cells are collected by centrifugation at 4500 rpm for 5 min under aseptic conditions.

[0051] Immediately resuspend the algal cells in an equal volume of fresh sterilized 15 mmol L -1 Continue culturing at 25°C in high nit...

Embodiment 3

[0055] The solid BG11 + Chlorella on plate medium was inoculated into sterilized BG11 + In the liquid culture medium, 25 ℃, 180rpm, culture 3 days, serve as primary seed liquid.

[0056] Inoculate the sterilized BG11 with a 10% (V / V) inoculum of the primary seed liquid + In liquid culture medium, cultured for 3 days, as the secondary seed solution. The culture conditions are the same as the seed solution.

[0057] The secondary seed solution was inoculated with 10% (V / V) inoculum in sterilized low nitrogen concentration of 3mmol L -1 BG11 + medium. The culture conditions were as follows: temperature 25°C, shaker speed 180 rpm.

[0058] When Chlorella grows to the end of the exponential phase, that is, on the 3rd day of the culture process, the algae cells are collected by centrifugation at 4500 rpm for 5 min under aseptic conditions.

[0059] Immediately resuspend the algal cells in an equal volume of fresh sterilized 12 mmol L -1 Continue culturing at 25°C in high nit...

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Abstract

The invention discloses a cultural method of obtaining heterotrophic chlorella cells with a high protein content. The operating method comprises the following steps of: heterotrophically culturing chlorella to an initial period of a growing logarithmic phase by using a nitrogen deficiency modified BG11+ organic culture medium; centrifugally collecting the algae cells in a sterile condition; and transferring the algae cells to a nitrogen sufficiency modified BG11+ organic culture medium to be continuously cultured to an initial period of a stable period to collect the algae with the high protein content. On the premise of guaranteeing the heterotrophic high biomass of the chlorella, the method improves the protein content in the heterotrophic chlorella living bodies, so that the output of the proteins is improved; and moreover, the produced single-cell proteins are reasonable in biochemical composition, and amino acid components meet the nutritional demand on foods and feeds. The method disclosed by the invention provides a novel technical route for improving the output of the single-cell proteins. The process has the advantages of being simple in process, simple to operate, short in production period, high in product quality and the like, and is easy for scaled production.

Description

technical field [0001] The invention belongs to the technical field of microalgae biological protein, in particular relates to a method for cultivating chlorella, in particular to a method for increasing the protein output of chlorella. Background technique [0002] Protein is the material basis of all life and an important part of cells. Life activities such as body growth, development, movement and reproduction are inseparable from protein. According to the 2011 survey by the Food and Agriculture Organization of the United Nations, the growth rate of population has far exceeded the growth rate of food protein supply. Lack of protein resources - This worldwide problem has existed for many years. With the continuous development of my country's economic construction and the continuous improvement of people's living standards, the total demand for protein is increasing year by year. In addition, my country is a populous country, and the growth of population also makes the pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12R1/89
Inventor 谢通慧曾宇张永奎
Owner SICHUAN UNIV
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