Resin embedding method of light transmittance biological tissues
A biological tissue, resin-packed technology, applied in the field of biomedical optical imaging, can solve the problems of small Z-axis imaging depth, unsuitable cutting imaging, long imaging time, etc., and achieves the effect of increasing depth, maintaining good fluorescence and shortening time.
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Embodiment 1
[0035] A resin embedding method for phototransparent mouse brain tissue, comprising the steps of:
[0036] (1) Place the rat brain fixed with formaldehyde solution in a tetrahydrofuran-water mixture (with a tetrahydrofuran content of 50%) for 12 hours; then replace the solution with a mixed solution with a tetrahydrofuran ratio of 80% for 1 hour; continue to replace until 1 h in pure THF, twice. The treated rat brain was then placed in dichloromethane for half an hour (eg figure 1 );
[0037] (2) Add the monomer benzyl methacrylate dropwise on the basic alumina (200-300 mesh) column chromatography column prepared in advance, flow out under the pressure of the air pump, and remove the polymerization inhibitor therein;
[0038] (3) In a serum bottle with a volume of 10ml, soak the treated rat brain in benzyl methacrylate monomer (2ml) (polymerization monomer), then add the initiator azobisisoheptanonitrile (4mg ) to fully dissolve it. Then wrap the bottle with tinfoil for sh...
Embodiment 2
[0042] Transparent embedding was performed using 2-acrylic acid-2-hydroxy-3-phenoxyacrylic acid as monomer.
[0043] (1) Place the rat brain fixed with formaldehyde solution in a tetrahydrofuran-water mixture (with a tetrahydrofuran content of 50%) for 12 hours; then replace the solution with a mixed solution with a tetrahydrofuran ratio of 80% for 1 hour; continue to replace until 1 h in pure THF, twice. The treated rat brain was then placed in dichloromethane for half an hour (eg figure 1 );
[0044] (2) Add the monomer 2-acrylic acid-2-hydroxyl-3-phenoxyacrylic acid dropwise on the basic alumina (200-300 mesh) column chromatography column prepared in advance, and flow out under the pressure of the air pump, Remove the polymerization inhibitor therein;
[0045] (3) The rat brain after transparent treatment was placed in a serum bottle with a capacity of 10 ml. Soak the treated rat brain in 2-acrylic acid-2-hydroxyl-3-phenoxyacrylic acid monomer (2ml) (polymerizable monom...
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