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A kind of seedling cultivation method of European aspen strain group

A technology of poplar and strains, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of low survival rate of cultivated seedlings, difficult survival of cultivated seedlings, unfeasible sterilization methods, etc.

Active Publication Date: 2018-09-11
FORESTRY RES INST OF HEILONGJIANG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Moreover, use the method in the "triploid European Populus without flocs rapid propagation system" for sterilization and tissue culture, and use the culture method in the reference "Triploid European Populus without flocs rapid propagation system" to reproduce the process Among them, the main problems are as follows: the sterilization method is not feasible, sterilize in 75% alcohol for about 30 to 60 seconds, sterilize in 0.1% mercury chloride for 5 to 10 minutes, rinse with sterile water for 3 to 5 times, and the browning rate reaches 85% to 100%, browning will make it difficult for cultivated seedlings to survive, and the higher the browning rate, the lower the survival rate of cultivated seedlings; use adventitious bud induction medium MS+6-BA0.5mg / L+0.05NAAmg / L is used for axillary bud induction, and the adventitious bud induction rate is only 14.2% to 25.8%. For the proliferation medium MS+6-BA0.5mg / L+0.05NAAmg / L, the plant proliferation rate is 2 to 3 times, and the average growth plant is 1.0~1.5cm, which eventually leads to low survival rate of cultivated seedlings

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0071] Cut off the twigs germinated from the roots of Populus eucalyptus, wash them under running water for 1 hour, remove the leaves of the twigs, cut the twigs with axillary buds into 1.0 cm stem segments, and treat the surface of the stem segments with a mass concentration of 70% ethanol solution. Disinfect for 10 ss, then rinse with sterile water for 3 times, then disinfect with 0.1% mercuric chloride for 2.0 min, rinse with sterile water for 3 times, and finally dry the sterile water with sterile filter paper. The two ends 1mm of the stem section with axillary buds were excised with a sterilized scalpel to obtain explants;

[0072] The explants were inoculated into the sterilized adventitious bud differentiation medium for differentiation culture to obtain the adventitious buds of Populus pubescens. The culture conditions included temperature 23°C, light time 12h / d, light intensity 3500Lx, and light source with trichromatic color temperature Cultivate for 30 days under th...

Embodiment 2

[0079] Cut off the twigs germinated from the roots of Populus eucalyptus, wash them under running water for 2 hours, remove the leaves of the twigs, cut the twigs with axillary buds into 1.5 cm stem segments, and treat the surface of the stem segments with a mass concentration of 75% ethanol solution. Disinfect for 15 seconds, then rinse with sterile water for 3 times, then disinfect with 0.12% mercury chloride for 4.0 minutes, rinse with sterile water for 3 times, and finally dry the sterile water with sterile filter paper. The two ends 2mm of the stem segment with axillary buds were excised with a sterilized scalpel to obtain explants;

[0080]The explants were inoculated into the sterilized adventitious bud differentiation medium for differentiation culture to obtain the adventitious buds of Populus pubescens. The culture conditions included temperature 26°C, light time 14h / d, light intensity 5000Lx, and light source with trichromatic color temperature Cultivate for 45 days...

Embodiment 3

[0087] Cut off the twigs germinated from the roots of Populus eucalyptus, wash them under running water for 1.5 hours, remove the leaves of the twigs, cut the twigs with axillary buds into 1.5 cm stem segments, and treat the stem segments with a mass concentration of 75% ethanol solution Disinfect the surface for 20 seconds, then rinse with sterile water for 3 times, then disinfect with 0.08% mercuric chloride for 3.5 minutes, rinse with sterile water for 3 times, and finally dry the sterile water with sterile filter paper. Sterilized scalpel excised the two ends of the stem with axillary buds 1.5mm to obtain explants;

[0088] The explants were inoculated into the sterilized adventitious bud differentiation medium for differentiation culture to obtain the adventitious buds of Populus pubescens. The culture conditions included temperature 24°C, light time 13h / d, light intensity 4000Lx, and light source with trichromatic color temperature Cultivate for 35 days under the conditi...

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Abstract

The invention provides a method for cultivating seedlings of Populus aspen strain group. After the stem section with axillary buds is soaked in alcohol solution, rinsed with sterile water, disinfected with disinfectant and rinsed again with sterile water, both ends of the stem section are removed. 1 to 2 mm, to obtain explants; inoculate the explants into the adventitious bud differentiation medium, differentiate and culture for 30 to 45 days, and obtain the adventitious buds of European aspen; inoculate the European aspen adventitious buds into the subculture proliferation medium Subculture to obtain 1.2-2.0 cm-high adventitious buds of Populus eucalyptus; transfer the 1.2-2.0 cm poplar eucalyptus buds to the adventitious bud rooting medium for rooting culture, and obtain regeneration tissue culture seedlings of Populus eucalyptus. By adopting the method for cultivating group seedlings of the present invention, the survival rate of the obtained group culture seedlings is 91.4-91.6%. The method for cultivating group seedlings of the present invention can take one stem section as the base, and can multiply to 10,000-100,000 group-cultured seedlings within one year after cultivation.

Description

technical field [0001] The invention belongs to the technical field of agriculture and forestry breeding methods, and in particular relates to a method for cultivating seedlings of European aspen strain groups. Background technique [0002] European Aspen is native to Altai, Tacheng, and the north slope of Tianshan Mountain in Xinjiang, China, to the Yili Mountains in the west. It is cold-resistant, light-loving, and cool-climate-loving. It can be widely cultivated and promoted in the same latitude regions in Northwest and Northeast my country. European aspen is a poplar tree species of the genus Populus. In nature, it mainly relies on seeds and root tillers for reproduction. The traits of the offspring of seed propagation will be separated, which cannot ensure the performance of excellent individual plant traits. Therefore, asexual reproduction is widely used in production. method. Asexual reproduction methods include cuttings, grafting, root tillers or tissue culture, etc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 白卉邢亚娟毕金鸽王艳敏卢慧颖郭成博李京兰岚李祺
Owner FORESTRY RES INST OF HEILONGJIANG PROVINCE