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Magnetic resonance, fluorescent double-developing nanometer gel, probe and preparation method of gel

A nanogel and magnetic resonance technology, applied in the field of biomedical materials, can solve problems such as the inability to realize two imaging methods at the same time, poor sensitivity and targeting, and clinical application limitations, and achieve good application prospects, high resolution, and low cost. low effect

Inactive Publication Date: 2017-06-30
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although magnetic resonance imaging can provide high-resolution tissue information and three-dimensional imaging structures, its sensitivity and targeting are poor, which limits its clinical application.
[0005] Existing probes only have single-function development capabilities, and cannot realize two development methods at the same time, so it is impossible to accumulate the advantages of the two development methods in one body

Method used

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  • Magnetic resonance, fluorescent double-developing nanometer gel, probe and preparation method of gel

Examples

Experimental program
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Effect test

Embodiment 1

[0034] A method for preparing a magnetic resonance fluorescence double imaging probe, comprising the following steps:

[0035] Step 1: preparing nanogel; including the following steps:

[0036] Step N1: Add chitosan to a certain amount of acetic acid solution under air-isolated conditions, and perform magnetic stirring at room temperature for 24 hours to fully dissolve; take 50 mL of chitosan solution and add it to a three-necked flask, nitrogen protection, After stirring at a constant temperature in an oil bath at 30°C, the initiator cerium ammonium nitrate was added; after the polysaccharide monomer reacted for 15 minutes, 0.640 mL of monomeric methyl acrylate was added with a micro-injector to react; and the reducing substance sensitive to the environment was added The cross-linking agent DADS; And continue to react 4h under nitrogen protection;

[0037] Step N2: Dialyzing the reacted gel to remove impurities therein;

[0038] Step 2: Coupling the prepared nanogel with NM...

Embodiment 2

[0042] A method for preparing a magnetic resonance fluorescence double imaging probe, comprising the following steps:

[0043] Step 1: preparing nanogel; including the following steps:

[0044] Step N1: Add carboxymethyl dextran to a certain amount of deionized water under the condition of isolated air, heat and magnetically stir for 24 hours to fully dissolve; take 50mL of carboxymethyl dextran solution into a three-necked flask, nitrogen protection, after stirring at a constant temperature in an oil bath at 50°C, add the initiator dibenzoyl peroxide; after reacting the polysaccharide monomer for 30 minutes, add 0.360 mL of monomer methyl methacrylate with a micro injector to react; and Add the cross-linking agent DADS sensitive to reducing substances; continue to react for 12h under the protection of nitrogen;

[0045] Step N2: Dialyzing the reacted gel to remove impurities therein;

[0046] Step 2: Coupling the prepared nanogel with NMR imaging molecules containing magnet...

Embodiment 3

[0050] A method for preparing a magnetic resonance fluorescence double imaging probe, comprising the following steps:

[0051] Step 1: preparing nanogel; including the following steps:

[0052] Step N1: Dissolving dextran in deionized water under heating and magnetic stirring to prepare a certain concentration of dextran solution. Take 25mL of dextran solution and put it into a three-necked flask, under nitrogen protection, after stirring at a constant temperature in an oil bath at 50°C, add the initiator N,N-xylylamine; after reacting for 30 minutes, add 0.275mL of hydroxyethyl acrylate mono body reaction; and N,N-methylenebisacrylamide was added for cross-linking; under the protection of nitrogen, the reaction was continued for 6h;

[0053] Step N2: Dialyzing the reacted gel to remove impurities therein;

[0054] Step 2: Coupling the prepared nanogel with NMR imaging molecules containing magnetic particles; take 42mg DOTA, dissolve it in 20mL TEMED / HCL buffer solution, add...

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Abstract

The invention discloses a magnetic resonance, a fluorescent double-developing nanometer gel, a probe and a preparation method of the gel. The method comprises the following steps: preparing a magnetic resonance developing functional nanometer gel by coupling a nanometer gel with the nuclear magnetic developing molecules to prepare a magnetic resonance and fluorescent double-developing functional nanometer gel by coupling with the fluorescent molecules. According to the invention, the double-developing of infrared developing and magnetic resonance developing is realized and complementary high-resolution magnetic resonance developing and high-sensitivity fluorescent developing are combined with each other. Besides, the nanometer gel has response to reducing matters and a weak acid environment, can be decomposed in a tumor environment so as to promote the mutation of fluorescent and magnetic resonance developing signals and has an excellent application prospect in high-sensitivity diagnosis for tumors.

Description

technical field [0001] The invention relates to a functional nano gel probe and a preparation method thereof, in particular to a magnetic resonance fluorescence double imaging functional nano gel probe and a preparation method thereof, belonging to the technical field of biomedical materials. Background technique [0002] Nanogel has a small particle size, a large specific surface area, strong adsorption and biological activity, and good stability. It is easy to gather in the liver and lymphatic system and has targeting. Nanogels can easily enter cells through the cell membrane, can realize the delivery of drugs, DNA, RNA, etc., and can protect drugs and the substances contained in them, improve delivery efficiency and bioavailability, and are widely used in the field of biomedicine. Therefore, it is of great significance to accurately monitor the running trajectory, drug release site, release mode, and therapeutic effect of functional nanogel carriers in vivo. [0003] How...

Claims

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Application Information

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IPC IPC(8): A61K49/18A61K49/10A61K49/00
CPCA61K49/0032A61K49/105A61K49/108A61K49/1803
Inventor 窦红静袁晓菁代振邦梅传能李文姚昊王灏
Owner SHANGHAI JIAO TONG UNIV
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