Preparation of up-conversion nanoparticles and method of showing occult blood fingerprint

A nanoparticle, latent fingerprint technology, applied in the field of detection, achieves the effect of good sensitivity and specificity, and fast color development

Inactive Publication Date: 2017-06-30
QINGDAO UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, sometimes analyzing ridge detail requires additional height identification

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  • Preparation of up-conversion nanoparticles and method of showing occult blood fingerprint
  • Preparation of up-conversion nanoparticles and method of showing occult blood fingerprint
  • Preparation of up-conversion nanoparticles and method of showing occult blood fingerprint

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[0018] A preparation method for upconversion nanoparticles, specifically comprising the steps of:

[0019] (1) Preparation of upconverting nanoparticle solution: Dissolve NaCl in secondary deionized water, slowly add oleic acid and ethanol with a volume ratio of 1:1 under strong stirring, continue stirring for 30 minutes, make it evenly mixed, and then add YbCl 3 , YCl 3 , GdCl 3 , ErCl 3 , and finally the NH added dropwise 4 F solution, stirred evenly, transferred to an autoclave, sealed, heated in an autoclave at 170-200°C for 8-10h, and naturally cooled to room temperature;

[0020] (2) Preparation of NaYF 4 :Yb 3+ / Er 3+ / Gd 3+ Nanoparticles: Centrifuge the obtained solution, wash it several times alternately with secondary deionized water and ethanol, add CTAB solution, stir evenly, heat to 30-35°C, heat for 30-50min, and then heat up to 65-75°C , heated for 25-50min, cooled to 30-25°C, centrifuged and washed several times with deionized water twice to obtain rod-...

Embodiment 1

[0024] At room temperature, dissolve 0.30-0.35g NaCl in secondary deionized water, slowly add 5mL oleic acid and 5mL ethanol under strong stirring, continue stirring for 30min to make it evenly mixed, then add 0.027-0.028g YbCl in turn 3 , 0.024~0.025g YCl 3 , 0.074~0.075g GdCl 3 , 0.0030~0.0035g ErCl 3 , and finally add 1 mL of 0.2 mol / L NH dropwise 4 F solution. Stir evenly, transfer to a 20mL reverse autoclave, seal, heat in an autoclave at 160°C for 10h, and cool naturally to room temperature. The obtained solution was centrifuged, washed alternately with secondary deionized water and ethanol several times, added 5ml of 0.2mol / L CTAB solution, stirred evenly, heated to 32°C for 30min, then heated to 68°C, and heated for 25min. Cool down to 30°C and wash several times with deionized water to obtain rod-shaped NaYF 4 :Yb 3+ / Er 3+ / Gd 3+ Nanoparticles. After the supernatant was removed by centrifugation, it was dissolved in chloroform, and reacted with 15.34 g of 1%...

Embodiment 2

[0026] At room temperature, dissolve 0.30-0.35g NaCl in secondary deionized water, slowly add 5mL oleic acid and 5mL ethanol under strong stirring, continue stirring for 30min to make it evenly mixed, then add 0.027-0.028g YbCl in turn 3 , 0.024~0.025g YCl 3 , 0.074~0.075g GdCl 3 , 0.0030~0.0035g ErCl 3 , and finally add 1 mL of 0.2 mol / L NH dropwise 4 F solution. Stir evenly, transfer to a 20mL reverse autoclave, seal, heat in an autoclave at 200°C for 6h, and cool to room temperature naturally. The obtained solution was centrifuged, washed alternately with secondary deionized water and ethanol several times, added 5ml of 0.2mol / L CTAB solution, stirred evenly, heated to 32°C for 30min, then heated to 68°C, and heated for 25min. Cool down to 30°C and wash several times with deionized water to obtain rod-shaped NaYF 4 :Yb 3+ / Er 3+ / Gd 3+ Nanoparticles. After the supernatant was removed by centrifugation, it was dissolved in chloroform, and reacted with 15.34 g of 1% ...

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Abstract

The invention belongs to the technical field of detection and in particular to preparation of up-conversion nanoparticles and a method of showing an occult blood fingerprint. Represented by a transmission electron microscope, the shape of each of the up-conversion nanoparticles is rod-like and the shapes of the nanoparticles are not changed if being connected to anti-hHb. A fluorescent spectra of the up-conversion nanoparticles are tested, and the up-conversion nanoparticles are excited in an exciting wavelength condition of 980nm to give out green fluorescence. By specifically combining the up-conversion nanoparticles with hemoglobin in an occult blood fingerprint component, more nanoparticles will be gathered in a fingerprint streakline region to form centralized gathering of the streakline region. The fingerprint streakline of a fingerprint sample in an excitation light source of 980nm is clearly displayed, so that a purpose of displaying the occult blood fingerprint fluorescently is achieved. The materials are easy to prepare, and the method is quick and easy to operate, and the synthetic process is green and environmental-friendly. The up-conversion nanoparticles provided by the invention are high in sensitivity and non-toxic, and do not damage potential information of bloody fingerprints.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to the preparation of up-conversion nanoparticles and a method for displaying occult blood fingerprints. Background technique [0002] Fingerprints are one of the most useful types of physical evidence because they represent the unique skin ridges on each individual finger, specifically the papillae pattern on the skin surface of the human hand, which is typically a hereditary trait. As a kind of physical evidence, tattoo has been hailed as "the first evidence" and has been recognized all over the world. It is widely used in investigating cases and authenticating criminals. How to find, visualize and collect it on a wide variety of objects at a crime scene is a key issue in the utilization of fingerprints. So far, many methods have been developed to improve the latent sweat fingerprint. However, a murder crime scene also leaves bloody fingerprints, which are often va...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/85C07K16/18G01N21/64G01N33/533
CPCC07K16/18C09K11/7791G01N21/6486G01N33/533
Inventor 陈丽华吕淑丽刘祥
Owner QINGDAO UNIV OF SCI & TECH
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