Chemical positioning method for tree plant catechin histiocyte

A technology of tissue cells and positioning methods, which is applied in the direction of optical testing of defects/defects, etc., can solve the problems of low sensitivity of dyeing agents and lack of specificity of dyeing agents, and achieve the effect of simple dyeing process and convenient operation

Inactive Publication Date: 2009-07-15
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the defects such as lack of specificity of staining agent, low sensitivity of staining agent, and fixing of materials before staining in the existing histochemical localization technology of phenolic substance

Method used

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  • Chemical positioning method for tree plant catechin histiocyte
  • Chemical positioning method for tree plant catechin histiocyte
  • Chemical positioning method for tree plant catechin histiocyte

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0037] Major equipment

[0038] 1. Cutting edge blade (Japan-US brand); 2. Slide glass; 3. Cover glass; 4. Dropper; 5. Petri dish with a diameter of 9 cm; 6. Sharp-nosed tweezers;

[0039] Materials and Reagents

[0040] 1. Materials:

[0041] (1) Fresh tea leaves and tender stems that have just grown within a week in spring or summer; (2) Tea root tips that have just grown; (3) Tea callus; (4) Fresh carrots.

[0042] 2. Main reagents:

[0043] (1) 1% vanillin concentrated hydrochloric acid solution: get 1g vanillin and dissolve in 100ml concentrated hydrochloric acid;

[0044] (2) clean water;

[0045] (3) 10% hydrochloric acid solution: get 26g concentrated hydrochloric acid concentration (36%) and add 10g water;

[0046] (4) softening agent: (50% alcohol: glycerin = 1: 1), that is: 50% alcohol solution and glycerin are mixed by volume 1: 1;

[0047] (5) 15% hydrofluoric acid: get 32g concentrated hydrofluoric acid concentration (47%) and add 15g water;

[0048] (6) F...

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Abstract

The invention relates to a tea catechin histocyte chemical positioning method, which can solves the problems of the present phenols tissue chemical positioning technique that: bad coloration specificity, slow coloration speed, not high sensitivity, incapability of tea catechin histocyte chemical positioning. The tea catechin histocyte chemical positioning method comprises the following steps: taking the ender tea fresh leaves or tender stalk or root tip or tea callus tissue; producing the slice; dropping 1%-3%(w/v)vanillin concentrated hydrochloric acid solution at one side of the tissue slice and sucking the solution at another side of the tissue slice using filter paper until the material is fully reddened; sucking the redundant vanillin concentrated hydrochloric acid solution using filter paper and drying the slice surface using a blower to obtain a colored slice; taking photomicrograph of the colored slice in 30 minutes after coloration and recording the shape of the colored slice. The coloration process is simple and the operation is convenient. Compared with the typic phenols substance such as ferric chloride, the vanillin acid solution has features of high coloration speed, high sensitivity, good specificity; not only the catechine in the tea organ tissue is positioned and but also the catechine in tea organelle is accurately positioned.

Description

technical field [0001] The invention relates to a method for chemical localization of tea tree catechin in tissue cells. Background technique [0002] Flavonoids in plants include chalcones, flavonoids, flavonols, flavanols, anthocyanins, proanthocyanidins, etc. The catechins in tea trees belong to the flavanols in flavonoids. [0003] At present, the staining agents used for the histochemical localization of flavonoids at home and abroad mainly include Fe 3+ or Fe 2+ Iron salt, Cr 2 o 7 2- , neutral red, methylene blue, toluidine, molybdate, vanillin acid solution, butanol sulfate dimethylaminocinnamaldehyde, etc. Most of the stains are broad-spectrum stains and lack specificity. [0004] Although vanillin acidic solution can react with dihydrochalcones, flavanols, dihydroflavonols, proanthocyanidins or condensed tannins to develop color, vanillin concentrated hydrochloric acid reagent is still widely used Quantitative detection of tea element) content [1] The reason...

Claims

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Application Information

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IPC IPC(8): G01N21/95
Inventor 刘亚军高丽萍夏涛
Owner ANHUI AGRICULTURAL UNIVERSITY
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