Chemical positioning method for tree plant catechin histiocyte

Abstract

The invention relates to a tea catechin histocyte chemical positioning method, which can solves the problems of the present phenols tissue chemical positioning technique that: bad coloration specificity, slow coloration speed, not high sensitivity, incapability of tea catechin histocyte chemical positioning. The tea catechin histocyte chemical positioning method comprises the following steps: taking the ender tea fresh leaves or tender stalk or root tip or tea callus tissue; producing the slice; dropping 1%-3%(w / v)vanillin concentrated hydrochloric acid solution at one side of the tissue slice and sucking the solution at another side of the tissue slice using filter paper until the material is fully reddened; sucking the redundant vanillin concentrated hydrochloric acid solution using filter paper and drying the slice surface using a blower to obtain a colored slice; taking photomicrograph of the colored slice in 30 minutes after coloration and recording the shape of the colored slice. The coloration process is simple and the operation is convenient. Compared with the typic phenols substance such as ferric chloride, the vanillin acid solution has features of high coloration speed, high sensitivity, good specificity; not only the catechine in the tea organ tissue is positioned and but also the catechine in tea organelle is accurately positioned.

Description

technical field [0001] The invention relates to a method for chemical localization of tea tree catechin in tissue cells. Background technique [0002] Flavonoids in plants include chalcones, flavonoids, flavonols, flavanols, anthocyanins, proanthocyanidins, etc. The catechins in tea trees belong to the flavanols in flavonoids. [0003] At present, the staining agents used for the histochemical localization of flavonoids at home and abroad mainly include Fe 3+ or Fe 2+ Iron salt, Cr 2 o 7 2- , neutral red, methylene blue, toluidine, molybdate, vanillin acid solution, butanol sulfate dimethylaminocinnamaldehyde, etc. Most of the stains are broad-spectrum stains and lack specificity. [0004] Although vanillin acidic solution can react with dihydrochalcones, flavanols, dihydroflavonols, proanthocyanidins or condensed tannins to develop color, vanillin concentrated hydrochloric acid reagent is still widely used Quantitative detection of tea element) content [1] The reason...

AI Technical Summary

Problems solved by technology

[0006] In order to solve the defects such as lack of specificity of staining agent, low sensitivity of staining agent, and fixing of materials before staining in the existing histochemical localization technology of phenolic substances, the present invention provides a method for histochemical localization of tea tree catechins , the method has the characteristics of strong color specificity, convenient operation, fast color development speed and high sensitivity

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