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Micro fluidic chip and preparation method and application thereof

A microfluidic chip and substrate technology, applied in the field of microfluidics, can solve problems such as chip product development troubles, expensive mold precision, etc., and achieve significant cost advantages, short reaction time, and small sample usage.

Active Publication Date: 2017-07-04
同昕生物技术(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The preparation of thermoplastic polymers by hot pressing or micro-injection molding has the advantages of being able to design complex channels to realize its separation or mixing functions, and the chip is easy to mass-produce, but it has high mold precision and high cost, and it needs to produce more than one million pieces to share the cost. Disadvantages such as cost have become one of the problems in the development of thermoplastic polymer microfluidic chip products

Method used

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  • Micro fluidic chip and preparation method and application thereof
  • Micro fluidic chip and preparation method and application thereof
  • Micro fluidic chip and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0038] The blood samples of 43 clinical samples (15 cases of heart failure patients, 28 cases of healthy physical examination) came from Tianjin First Central Hospital. Embodiment 1, a kind of BNP microfluidic detection reagent and preparation method thereof

[0039] The microfluidic chip of this embodiment was prepared by the following method:

[0040] (1) The BNP microfluidic detection reagent consists of a three-layer structure, namely a cover sheet, an adhesive layer and a substrate. The cover sheet is a transparent acrylic plate (thickness 1mm), the adhesive layer is double-sided adhesive tape with a thickness of 0.115mm, and the base sheet is a non-transparent acrylic plate (thickness 2mm). The above three-layer structures are all laser engraved or cut by machine tools. The chip structure is as follows: Figure 1-2 shown.

[0041] (2) Cover sheet structure such as image 3 As shown, it includes sample loading area, speed control area 1-2, exhaust hole, and microchann...

Embodiment 2

[0050] Embodiment 2, a kind of BNP microfluidic detection reagent and preparation method thereof

[0051] Except that the preparation methods of the following structural parts are different, other preparation methods are the same as in Example 1.

[0052] (1) cover sheet

[0053] a. The thickness is 0.5mm;

[0054] b. Speed ​​control area 1: use stamping ink (Dongguan Cairong Ink Co., Ltd.) to draw 6 lines (width 1mm) (spacing 1mm);

[0055] c. Speed ​​control area 2: Use polyether modified silicone oil (Jining Huakai Resin Co., Ltd.) to draw 6 lines (width 1mm) (spacing 1mm);

[0056] d. Microchannel control line: use polyether modified silicone oil (Jining Huakai Resin Co., Ltd.) to draw a line (width 2mm), and the distance between two microchannel control lines (microfluidic channel) is 3mm;

[0057] (3) The thickness of the substrate is 4mm, and the structure is as follows Figure 4 As shown, it includes sample loading area, speed control area 1-2, microchannel control...

Embodiment 3

[0058] Example 3, a BNP microfluidic detection chip and its preparation method

[0059] Except that the preparation methods of the following structural parts are different, other preparation methods are the same as in Example 2.

[0060] (1) The cover slip only has a sample loading area and an air vent, without the speed control area and microchannel control line in the cover slip of Example 2; the thickness of the cover slip is 2mm.

[0061] (2) The speed control area 1 of the substrate is processed by machine tools, and it is a groove with a width of 0.01mm and a depth of 0.05mm. Polyether modified silicone oil is injected into the groove, and the groove interval is 0.5mm; the thickness of the substrate is 0.5mm.

[0062] (3) The speed control area 2 of the substrate is processed by machine tools, and is a groove with a width of 0.5 mm and a depth of 0.3 mm. Marker ink is injected into the groove, and the distance between the grooves is 0.8 mm.

[0063] (4) The microchannel c...

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Abstract

The invention provides a micro fluidic chip, which belongs to the technical field of micro fluidic. The chip comprises a substrate, the surface of the substrate comprises at least one reaction zone; a target molecule combination object is arranged in the reaction zone, and is used for capturing and / or combining target molecules in a to-be-measured liquid sample and is subjected to a combination reaction in the reaction zone; according to a liquid flow direction, in the reaction zone, and / or, at least one speed control zone is arranged at downstream position close to the reaction zone, a hydrophobic agent is arranged in the speed control zone, and is used for intercepting the target molecule in the reaction zone for full reaction, continuous flowing of the liquid sample cannot be completely obstructed, so that the control on flow velocity of the liquid sample is realized. The invention also provides an application of the chip and its preparation method thereof. The chip has the advantages of good repeatability, high sensitivity, and low cost.

Description

technical field [0001] The invention belongs to the field of microfluidic technology, and in particular relates to a microfluidic chip and its preparation method and application. Background technique [0002] Microfluidic technology is a science and technology characterized by the manipulation of fluids in micron-scale spaces. Microfluidic chips have the ability to shrink the basic functions of laboratories such as biology and chemistry to a chip of a few square centimeters, also known as chips. Laboratory (Labon Chip). In the past ten years, this technology has been gradually applied in the field of analysis and detection, which has the advantages of cost saving, time saving, easy operation, and high-throughput detection. [0003] Microfluidic technology involves many interdisciplinary subjects. Due to different application fields or research directions, microfluidic chip manufacturing methods and materials are diverse. For example, patent CN104155462A, patent CN103278643...

Claims

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Application Information

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IPC IPC(8): B01L3/00G01N33/53
CPCB01L3/502746B01L3/502761B01L2200/10B01L2200/143B01L2200/148B01L2300/165G01N33/5302
Inventor 彭京胜焦守恕李全吴凡
Owner 同昕生物技术(北京)有限公司
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