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Mouse mammary gland syringe needle and application thereof

An injection needle and mammary gland technology, which is applied in the field of experimental zoology and pathology, can solve the problems of difficulty in ensuring that the injection needle penetrates into mammary gland tissue, high price, mammary gland damage, etc., and achieves convenient and quick test means, easy-to-obtain materials, and low price. Effect

Inactive Publication Date: 2017-07-07
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The first is subcutaneous injection. Due to the lamellar structure of the mouse mammary gland, it is difficult to ensure that the injection needle penetrates into the mammary gland tissue with subcutaneous injection, and it will cause damage to the mammary gland, cause inflammation, and mislead the experimental results.
The second is to use a micro-injection needle to inject after cutting off the nipple. The disadvantage of this method is that the diameter of the micro-injection needle is large, it is difficult to enter the milk pool through the teat duct, and the hard needle is easy to cause damage to the breast tissue.
In addition, imported micro-syringes are used in individual studies, which are expensive and difficult to obtain, which is not conducive to the rapid development of research work

Method used

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  • Mouse mammary gland syringe needle and application thereof
  • Mouse mammary gland syringe needle and application thereof
  • Mouse mammary gland syringe needle and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. Experiment of LPS-induced mammary gland inflammatory response in mice

[0029] Step 1: Take a mini alcohol lamp and some 200μL sterilized pipette tips (polypropylene and other plastic materials); light the alcohol lamp, hold the end of the pipette tip with one hand, and hold the pipette with steel tweezers with the other hand Heat the tip of the pipette tip on an alcohol lamp at 5mm-10mm; when the heated part of the pipette tip is melted and deformed, quickly move out of the flame range, and at the same time quickly stretch your hands to both sides, then the pipette can be The heated part of the pipette tip is pulled into a very thin tubular structure; after the pipette tip is cooled and shaped, loosen the steel tweezers, and cut the thin tube with a scalpel at a diameter of 0.5mm-1.0mm, and then use It is advisable to keep a 3-6cm thin tube on the pipette tip to make a breast injection needle, see figure 1 .

[0030] Step 2: intraperitoneally inject mice w...

Embodiment 2

[0039] Example 2. Experiment of LTA-induced mammary gland inflammatory response in mice

[0040] In the implementation process, it is also possible not to cut the nipple of the mouse, but after removing the cutin of the nipple of the mouse, use the tip of the mammary gland injection needle (the incision of the thin tube is an inclined plane) to perform a certain number of piercing operations on the end surface of the nipple until the injection needle The tip enters the milk duct from the foramen of the nipple. This method can satisfy the experimental research that needs multiple injections.

[0041] The steps of this embodiment are basically the same as in Example 1, except that the injected liquid is lipoteichoic acid (LTA), and after the injection is completed (after step five), the mouse is put back into a clean cage, placed on its back to avoid Wound infection. Thereafter, injections were performed every 2 days, and a total of 3 injections were performed until the end of...

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Abstract

The invention discloses a mouse mammary gland syringe needle and application thereof. The mammary gland syringe needle is prepared by performing hot stretch deformation on a head portion of a pipettor tip and truncating a fine tubular structure formed by the stretching portion. Materials of the prepared mammary gland syringe needle are easily available, the injection effect is ideal, research materials can be provided for mammary gland pathology research. Compared with a traditional injection method that easily causes mammary gland mechanical damage or needs an expensive micro-syringe, the mouse mammary gland syringe needle has wider application value.

Description

technical field [0001] The invention relates to the fields of experimental zoology and pathology, in particular to a mammary gland injection device that does not cause mechanical damage to mouse mammary glands and a non-therapeutic mammary gland injection method. Background technique [0002] Mice belong to the phylum Vertebrate, Class Mammalia, Order Rodentia, Family Muridae, genus Mice, and species Mus musculus, and are currently the most widely used, most thoroughly studied, and most diverse mammalian experimental animals. However, because the mouse mammary gland is sandwiched between the skin and the peritoneum, it is in the shape of a thin sheet, and the mouse nipple is extremely small, and the nipple hole is still invisible under the dissecting microscope, which increases the difficulty of using mice to conduct mammary gland experiments in vivo. [0003] Currently, there are three common methods for mouse mammary gland injection. The first is subcutaneous injection. D...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D7/00A61M5/32
CPCA61D7/00A61M5/3286A61M2207/00A61M2210/1007A61M2250/00
Inventor 高明清陈青张涌杨威
Owner NORTHWEST A & F UNIV
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