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A kind of preparation method of ganoderma enoic acid b

A technology of ganoderma enoic acid and ganoderma lucidum fruiting body is applied in the field of preparation of ganoderma enoic acid B, which can solve the problem of not finding a preparation method of ganoderma enoic acid B, and achieve the effects of low cost, low sample decomposition rate and little pollution.

Active Publication Date: 2020-11-20
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Through literature search, no preparation method of ganocidin B has been found at present

Method used

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  • A kind of preparation method of ganoderma enoic acid b
  • A kind of preparation method of ganoderma enoic acid b
  • A kind of preparation method of ganoderma enoic acid b

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] (1) Take 50 kg slices of Ganoderma lucidum fruiting bodies, put them into the pilot extraction equipment, add 8 times the volume of 87% absolute ethanol to extract twice, the first time for 2 hours, and the second time for 1 hour. The extraction temperatures were all 65°C. The two extracts were combined, heated and concentrated in a cooking tank, and then heated and concentrated with a 20L rotary evaporator to obtain the fruiting body extract of Ganoderma lucidum.

[0039] The fruiting body extract of Ganoderma lucidum is eluted with D101 macroporous resin, eluted with 60% ethanol, and eluted with 3-5 times the volume of the solution. Concentrate the eluted fraction to obtain a fraction rich in ganocidinic acid B.

[0040] (2) Prepare n-hexane: ethyl acetate: methanol: water (5:5:2:9, V / V) two-phase solvent system in a separatory funnel. Pump the upper phase (stationary phase) into the high-speed countercurrent chromatograph, and after the pipeline is filled with the ...

Embodiment 2

[0042] (1) Take 50 kg slices of Ganoderma lucidum fruiting bodies, put them into the pilot extraction equipment, add 8 times the volume of 87% absolute ethanol to extract twice, the first time for 2 hours, and the second time for 1 hour. The extraction temperatures were all 65°C. The two extracts were combined, heated and concentrated in a cooking tank, and then heated and concentrated with a 20L rotary evaporator to obtain the fruiting body extract of Ganoderma lucidum.

[0043] The fruiting body extract of Ganoderma lucidum is eluted with D101 macroporous resin, eluted with 60% ethanol, and eluted with 3-5 times the volume of the solution. Concentrate the eluted fraction to obtain a fraction rich in ganocidinic acid B.

[0044] (2) Prepare n-hexane:ethyl acetate:methanol:water (5:5:2:9, V / V) two-phase solvent system in a separatory funnel. Pump the upper phase (stationary phase) into the high-speed countercurrent chromatograph, and after the pipeline is filled with the upp...

Embodiment 3

[0046] Use high-performance liquid chromatography to detect the content of ganoderma enoic acid B in the test tubes with the peak area normalization method, combine the test tubes with a content of more than 88%, concentrate by rotary evaporation, and then concentrate and dry by vacuum centrifugation to obtain high-purity ganoderma enoic acid B .

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Abstract

The invention discloses a method for preparing ganoderenicacid B by means of separation. The method comprises the following steps: (1) extracting and enriching ganoderenicacid B: adding ganoderma lucidum sporocarp into 75-95% ethanol solution, extracting by heating, and concentrating extract; carrying out gradient elution and absorption on concentrate by using ethanol and macroporous resin, and then carrying out vacuum concentration; (2) separating and purifying by using a high-speed counter-current chromatography so as to prepare the ganoderenicacid B; separating the ganoderenicacid B by using the high-speed counter-current chromatography, then adopting a high performance liquid chromatography to perform analysis and detection, collecting, concentrating, drying, and separating to obtain the ganoderenicacid B. The method is simple in operation, less in pollution and low in cost; the ganoderenicacid B is high in yield and purity.

Description

technical field [0001] The invention belongs to the technical field of natural medicines, and in particular relates to a preparation method of ganoderma enoic acid B. [0002] technical background [0003] Ganoderic acid B is a triterpenoid compound, CAS No. 100665-41-6, molecular formula C 30 h 42 o 7 , the molecular weight is 514.65028, and the molecular structure formula is as follows: [0004] [0005] Ganoderma lucidum triterpenes are secondary metabolites of Ganoderma lucidum, which have various pharmacological activities. It has been reported in the literature that Ganoderenoic acid B has a strong activity of inhibiting p388, BEL-7402, SGC-7901, HeLa and other tumor cells in vitro. Therefore, it has the potential to solve the problem of multidrug resistance mediated by ABCB1 gene. [0006] Through literature search, no preparation method of ganocidin B has been found at present. Contents of the invention [0007] The object of the present invention is to pro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J9/00
CPCC07J9/005
Inventor 张劲松冯娜钱丁寅冯杰唐庆九杨焱刘艳芳吴迪颜梦秋唐传红刘方
Owner SHANGHAI ACAD OF AGRI SCI