Specific monoclonal antibody hybridoma cell strain WXX-2 of antiprostaglandin F2alpha and application thereof

A hybridoma cell line and monoclonal antibody technology, which is applied in the field of clinical detection, can solve the problems of expensive and complicated operations, and cannot achieve large-scale rapid detection on site, and achieve good detection sensitivity and affinity, and simplify the synthesis steps.

Active Publication Date: 2017-07-07
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above detection methods can perform quantitative analysis and have a low detection limit, but usually require expensive instruments and complicated operations, and the pretre

Method used

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  • Specific monoclonal antibody hybridoma cell strain WXX-2 of antiprostaglandin F2alpha and application thereof
  • Specific monoclonal antibody hybridoma cell strain WXX-2 of antiprostaglandin F2alpha and application thereof
  • Specific monoclonal antibody hybridoma cell strain WXX-2 of antiprostaglandin F2alpha and application thereof

Examples

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Embodiment 1

[0019] Example 1 Anti-prostaglandin F 2α Preparation of Specific Monoclonal Antibody Hybridoma Cell Line WXX-2

[0020] (1) Synthesis of complete antigen:

[0021] Synthesis of complete antigen: take 4mg hapten F 2α , then add 5.0 mg EDC (1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride) and 3.7 mg NHS (N-hydroxysuccinimide), using DMF (N, N-dimethylformamide) dissolved, stirred at room temperature, and activated for 6h; another 15mg BSA (bovine serum albumin) was dissolved in 3mL, 0.05M, pH9.6 CBS (carbonate buffer solution) solution; the above The activation solution was added dropwise to the BSA solution, stirred overnight at room temperature, and then the immunogen was taken out and dialyzed in PBS for 3 days, and stored at -20°C.

[0022] (2) Animal immunization: healthy BALB / c mice aged 6-8 weeks were selected for immunization. prostaglandin F 2α After the complete antigen (1 mg / mL) was emulsified with an equal amount of Freund's adjuvant, the BALB / c mice...

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Abstract

The invention relates to a specific monoclonal antibody hybridoma cell strain WXX-2 of antiprostaglandin F2alpha and application thereof and belongs to the technical field of clinical detection. The specific monoclonal antibody hybridoma cell strain WXX-2 of antiprostaglandin F2alpha provided by the invention has been preserved in CGMCC with the preservation number of CGMCC No. 13087. The specific monoclonal antibody of antiprostaglandin F2alpha is secreted by the specific monoclonal antibody hybridoma cell strain WXX-2 of antiprostaglandin F2alpha. The hybridoma cell strain WXX-2 can secrete a monoclonal antibody which has relatively good specificity and relatively high sensitivity on prostaglandin F2alpha, and the 50% inhibition concentration IC50 on the prostaglandin F2alpha is 1.80 [mu]g/L. The specific monoclonal antibody can be used for specific detection of the prostaglandin F2alpha in clinical detection.

Description

technical field [0001] An anti-prostaglandin F strain of the present invention 2α Specific monoclonal antibody hybridoma cell line WXX-2 and its application, involving prostaglandin F 2α The hybridoma cell line and the anti-specific monoclonal antibody produced therein belong to the technical field of clinical testing. Background technique [0002] Prostaglandins (PGs) are a class of unsaturated hydroxy fatty acids with similar chemical composition and extensive biological activities. The PGs family mainly includes: Prostaglandin D 2 , prostaglandin E 2 , prostaglandin F 2α , prostaglandin I 2 and TXA 2 , Prostaglandins are present in very small amounts in tissues, but they are highly active. The endometrium is one of the important sites for the synthesis of prostaglandins, and the E of prostaglandins 2 and F 2α It is the main arachidonic acid product synthesized by the endometrium. [0003] At present, prostaglandins are mostly detected by immunoaffinity chromatog...

Claims

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Application Information

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IPC IPC(8): C12N5/20C07K16/26C07K14/765G01N33/88G01N33/577C12R1/91
CPCC07K14/765C07K16/26C07K19/00G01N33/577G01N33/88
Inventor 刘丽强王忠兴胥传来匡华徐丽广马伟吴晓玲宋珊珊胡拥明
Owner JIANGNAN UNIV
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