Method used for extracting antibacterial composition from limulus reagent production waste materials

Abstract

The invention relates to a method used for extracting an antibacterial composition from limulus reagent production waste materials. The method comprises following steps: 1, raw material treatment is carried out, wherein a lower layer precipitate waste material obtained in centrifugation of an emulsified product in limulus reagent production is collected, and is taken as a raw material I, and discarded blood plasma in limulus reagent production is collected, and is taken as a raw material II; 2, extraction of a tachyplesin crude extracted solution is carried out, wherein acidolysis is carried out, pH is adjusted so as to remove a precipitate product, and thermal denaturation is carried out; 3, preparation of a hemocyanin crude extracted solution from blood plasma is carried out; 4, preparation of a SOD crude extracted solution is carried out; 5, 60 to 80 parts of the tachyplesin crude extracted solution,10 to 20 parts of the hemocyanin crude extracted solution, and 10 to 20 parts of the SOD crude extracted solution are mixed, pH value is adjusted to 4 to 5, and filtering is carried out so as to obtain the antibacterial composition. According to the method, the crude extracted solutions are prepared rapidly and conveniently at low cost; the antibacterial composition is capable of inhibiting growth of a plurality of kinds of bacteria and fungus; compared with conventional chemical antibacterial products, the antibacterial composition possesses following advantages: the antibacterial composition is safe, mild, and effective, no toxic or side effect is caused, and generation of drug resistance and drug allergy caused by conventional antibacterial products can be avoided.

Description

technical field [0001] The invention relates to the technical field of re-extraction of Limulus reagent waste, in particular to a method for extracting an antibacterial composition from Limulus reagent production waste. Background technique [0002] Limulus, also known as horseshoe crab, is a large benthic arthropod living in the ocean. It has a history of more than 400 million years since the Ordovician in the early Paleozoic Era. It is a "living fossil" with a unique evolutionary status in the animal kingdom. one. Limulus is an excellent material for invertebrate hematology research, and it is also the main raw material for the development and utilization of Limulus resources and the development of Limulus reagents. It has been highly valued by scholars in Japan, the United States and other countries. With the study of the immune function of Limulus hemolymph and the immunological comparison with other invertebrate hemolymph, especially many antibacterial and antiviral ac...

AI Technical Summary

Problems solved by technology

[0014] If the limulus peptide is directly extracted from the limulus cells, compared with the limulus peptide extracted from the limulus reagent waste, the finally obtained limulus peptide content is higher, but due to the conflict with the raw material (lysus cells) in the extraction of the above-mentioned limulus reagent, makes the extraction itself expensive

If the limulus peptide is directly extracted from the waste of the limulus reagent, the content of the limulus peptide is relatively low, and the subsequent purification steps are cumbersome and the process cost is high. is a big difficulty

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