Kit for jointly detecting acute myocardial infarction biomarkers

A technology of acute myocardial infarction and biomarkers, applied in biological testing, biomaterial analysis, measuring devices, etc., to achieve high clinical test application value and improve the effect of diagnosis rate

Inactive Publication Date: 2017-07-14
中国人民解放军第四五八医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no rapid combined detection of cardiac troponin I (cTn I), creatine kinase isoenzyme (CK-MB) and myoglobin (MYO) at the same time in acute myocardial infarction. Infarction biomarker kit report

Method used

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  • Kit for jointly detecting acute myocardial infarction biomarkers
  • Kit for jointly detecting acute myocardial infarction biomarkers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] A kit for joint detection of biomarkers of acute myocardial infarction, comprising: working microsphere probe solution, human cTn I series gradient standard solution, human CK-MB series gradient standard solution, human MYO series gradient standard solution, Triple acute myocardial infarction biomarker monoclonal antibody solution; 100× diluted streptavidin phycoerythrin solution and 200× diluted biotinylated mouse anti-human secondary antibody IgG solution;

[0048] Described working microsphere probe solution is made with following method:

[0049] (1) Take the carboxyl fluorescence-encoded microspheres coded No. 35, 42 and 52 and place them in three centrifuge tubes, resuspend with 100 μL of PBS with pH 7.4, so that the concentration of the carboxyl fluorescence-encoded microspheres is 1.25 ×10 7 Microspheres / mL, add 100 μL PBS with pH 8.0 0.1 mol / L, add freshly prepared 10 μL EDC aqueous solution with a concentration of 50 mg / mL and 10 μL S-NHS aqueous solution wit...

Embodiment 2

[0064] A kit for joint detection of biomarkers of acute myocardial infarction, comprising: working microsphere probe solution, human cTn I series gradient standard solution, human CK-MB series gradient standard solution, human MYO series gradient standard solution, Triple acute myocardial infarction biomarker monoclonal antibody solution; 100× diluted streptavidin phycoerythrin solution and 200× diluted biotinylated mouse anti-human secondary antibody IgG solution;

[0065] Described working microsphere probe solution is made with following method:

[0066] (1) except that the stirring time at room temperature is 10min, other steps (1) in the working microsphere probe solution preparation method of Example 1; Add 10 μg cTn I to the tube; add 10 μg CK-MB to the centrifuge tube containing the activated No. 42 carboxy fluorescence-encoded microspheres; add 10 μg MYO to the centrifuge tube containing the activated No. 52 carboxy fluorescence-encoded microspheres ; Add PBS with pH...

Embodiment 3

[0075] A kit for joint detection of biomarkers of acute myocardial infarction, comprising: working microsphere probe solution, human cTn I series gradient standard solution, human CK-MB series gradient standard solution, human MYO series gradient standard solution, Triple acute myocardial infarction biomarker monoclonal antibody solution; 100× diluted streptavidin phycoerythrin solution and 200× diluted biotinylated mouse anti-human secondary antibody IgG solution;

[0076] Described working microsphere probe solution is made with following method:

[0077] (1) Step (1) in the preparation method of the working microsphere probe solution with Example 1 except that the stirring time at room temperature is 30min; Add 10 μg cTn I; add 10 μg CK-MB to the centrifuge tube containing the activated No. 42 carboxy fluorescence-encoded microspheres; add 10 μg MYO to the centrifuge tube containing the activated No. 52 carboxy fluorescence-encoded microspheres; Add PBS with pH 7.4 to make...

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Abstract

The invention discloses a kit for jointly detecting acute myocardial infarction biomarkers. The kit comprises a working microsphere probe solution, a human cTn I series gradient standard substance solution, a human CK-MB series gradient standard substance solution, a human MYO series gradient standard substance solution, a triple acute myocardial infarction biomarker monoclonal antibody solution, a 100 * diluted streptavidin phycoerythrin solution and a 200 * diluted biotinylated mouse anti-human secondary antibody IgG solution. The kit establishes a high-throughput detection method of an AMI biomarker, can simultaneously and rapidly detect cTn I, CK-MB and MYO within 2 h, is suitable for the early diagnosis of AMI and finding of the AMI, improves the diagnosis rate of the AMI, and has high clinic examination application values in the early screening, disease condition monitoring, curative effect observation and recurrence judgment prediction and other stages of the AMI.

Description

technical field [0001] The invention relates to a kit for detecting biomarkers, in particular to a kit for combined detection of biomarkers for acute myocardial infarction. Background technique [0002] One of the most important factors leading to the death of patients in coronary heart disease is acute myocardial infarction (AMI), which is a cardiovascular disease that seriously endangers human health. [1] . AMI is a sharp reduction or interruption of coronary artery blood supply on the basis of coronary artery disease, resulting in severe and persistent acute ischemia of the corresponding myocardium, resulting in myocardial necrosis in a short period of time [2] . Therefore, timely diagnosis and reperfusion therapy in the early stage of AMI can significantly reduce the mortality rate and improve the prognosis. According to the diagnostic criteria established by the Cardiovascular Branch of the Chinese Medical Association, at least two of the three diagnostic criteria sh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/573
CPCG01N33/573G01N33/6887G01N33/6893G01N2800/324
Inventor 郑凤娇郑小玲
Owner 中国人民解放军第四五八医院
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