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Biological compound degradation agent in anaerobic condition and application thereof

A technology of anaerobic conditions and degradation agents, which is applied in the direction of microorganisms, microorganisms, biological water/sewage treatment, etc., can solve the problems that are not many and do not meet the secondary standard of sewage discharge.

Active Publication Date: 2017-07-18
BEIJING UNIV OF AGRI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on the degradation of low ammonia nitrogen concentration below 500mg / L is the most, and it can be degraded to 90% to 100%, which generally meets the secondary discharge standard of sewage; but there are not many studies on the degradation of high ammonia nitrogen concentration above 500mg / L , the maximum concentration studied so far is 1500mg / L, the degradation efficiency is only 30%, and has not reached the secondary standard for sewage discharge; and for the research on the degradation of ammonia nitrogen with a concentration above 1500mg / L, especially for 2000 or 3000mg / L There are very few studies on high concentrations of ammonia nitrogen

Method used

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  • Biological compound degradation agent in anaerobic condition and application thereof
  • Biological compound degradation agent in anaerobic condition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Separation and purification of bacteria and fungi in biocomposite degradation agents

[0021] Take 10ml of the landfill leachate from Nangong Garbage Composting Field in Beijing, inoculate it into a 250ml Erlenmeyer flask containing 100ml of sterilized enrichment medium, shake it well, and culture it with shaking at a temperature of 28°C and a rotational speed of 160r / min. Enriched for 7 days, supplemented every other day (NH 4 ) 2 SO 4 , to eliminate the inability to utilize NH 4 + -N microorganisms, the preparation of the enrichment medium is as follows: Brown sugar 19.02~29.68g / L, K 2 HPO 4 14g / L, KH 2 PO 4 6g / L, (NH 4 ) 2 SO 4 4.72~6g / L, MgSO 4 ·7H 2 O 0.2g / L, trace elements 2mL / L, the rest is water.

[0022] Take 1ml of the bacterial solution in the enrichment medium and spread it on the separation medium plate, culture it upside down in an incubator at 28°C, pick the single colonies grown on the plate for repeated isolation and purification, and...

Embodiment 2

[0038] Example 2: Experimental verification of the degradation of high-concentration ammonia nitrogen by biocomposite degradation agents

[0039] The biocomposite degradation agent that embodiment 1 prepares is connected in the Erlenmeyer flask (2 parallel samples, 1 contrast) of the screening culture medium of the initial ammonia nitrogen concentration of 3000mg / L after 100ml sterilization, at 28 ℃ of temperature, pH value 7. Aerobic culture in a shaking table with a rotating speed of 160r / min, the maximum degradation rate is 24%, and the degradation effect is not very ideal; but anaerobic culture is carried out in an incubator with a temperature of 30°C, and Nessler's reagent colorimetric method is used for colorimetry every day. The ammonia nitrogen in the 3 bottles of liquid culture medium was continuously monitored, and the highest dropped to more than 90%.

Embodiment 3

[0040] Embodiment 3: the application of biocomposite degradation agent

[0041] The samples retrieved from the large tower and small tower of Beijing Nangong Biological Deodorization Tower were tested for ammonia nitrogen index: the ammonia nitrogen content of the odor washing liquid in the big tower was 1713mg / L, the pH value was 6.30, and the ammonia gas content at the gas outlet was 8.26mg / m3 3 , the ammonia nitrogen content of the small tower odor washing liquid is 2471mg / L, the pH value is 6.24, and the ammonia gas content at the gas outlet is 8.95mg / m 3 .

[0042] Add the biocomposite degradation agent into the odor washing solution of Nangong large biological deodorization tower and small biological deodorization tower according to the inoculation amount of 1%, and cultivate it in an anaerobic environment at 32°C for 5-10 days, and the odor of the large biological deodorization tower is washed The ammonia nitrogen in the liquid is degraded to 133.23mg / L, the pH value i...

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Abstract

The invention relates to a biological compound degradation agent in an anaerobic condition and application thereof. The biological compound degradation agent comprises Citrobacter sp H5-XJ, the preservation number of which is CGMCC No.13504 and Galactomyces sp H5-ZJ, the preservation number of which is CGMCC No.13378. Both the Citrobacter sp H5-XJ and the Galactomyces sp H5-ZJ have been preserved in CGMCC on 26th, December, 2016. The invention further discloses application of the biological compound degradation agent in the anaerobic condition. The biological compound degradation agent provided by the invention has the advantages that the biological compound degradation agent can degrade high concentration ammonia and nitrogen in the anaerobic condition; no nitrite nitrogen and nitrate nitrogen are accumulated; nitration and denitration are performed synchronously, so that the ammonia and nitrogen degradation efficiency reaches 90% above.

Description

technical field [0001] The invention relates to a biocomposite degradation agent, in particular to a biocomposite degradation agent under anaerobic conditions and an application thereof. Background technique [0002] Water is an important resource for human beings and one of the basic substances for all living things to survive. With the advancement of industrialization and the continuous development of social economy, water pollution is becoming more and more worrying. Among them, ammonia nitrogen is one of the main pollution sources of water pollution. The content of ammonia nitrogen in sewage directly affects the quality of water and the degree of water odor. According to the provisions of the second category of pollutants in GB8978-1996 "Integrated Wastewater Discharge Standard", the maximum allowable discharge The secondary standard limit of ammonia nitrogen content should be less than 50mg / L. In most studies, the initial concentration of ammonia nitrogen is generally 1...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C02F3/34B01D53/84C12R1/01C12R1/645
CPCB01D53/84C02F3/34C02F3/347C02F3/348C02F2103/06C12N1/14C12N1/20C12N1/145C12N1/205C12R2001/01C12R2001/645Y02A50/20
Inventor 靳永胜秦文郝芳华靳静晨罗胜南杨祎尚润东张闻天孙楠韩扬
Owner BEIJING UNIV OF AGRI
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