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Bletilla striata ethyl acetate extract and application thereof

A technology of ethyl acetate extraction and ethyl acetate, applied in the field of medicine, can solve problems such as unclear composition and mechanism of action

Inactive Publication Date: 2017-07-21
SICHUAN NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Baiji has the effect of treating lung injury, hemoptysis, epistaxis, etc., but the specific components and mechanism of action are not yet clear.

Method used

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  • Bletilla striata ethyl acetate extract and application thereof
  • Bletilla striata ethyl acetate extract and application thereof
  • Bletilla striata ethyl acetate extract and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1 The preparation method of whitening and ethyl acetate extract of the present invention

[0023] Get 500g of basil, pulverize with a pulverizer to make coarse powder, pass the No. 3 sieve of the Pharmacopoeia, soak for 24h with 95% ethanol, and extract the coarse powder after soaking twice with an ultrasonic extractor, the ultrasonic power is 400W, each After 30 minutes, the extracts were combined and filtered, and the filtrate was concentrated into an extract by a rotary evaporator at 60°C under reduced pressure, and dried in an oven to obtain a crude extract, which was dried in vacuum to obtain a freeze-dried powder, and the extract was obtained by weighing. Rate.

[0024] Accurately weigh 100g of lyophilized powder of white and ethanol extract, disperse in 500ml of distilled water respectively, add petroleum ether according to the ratio of ethanol extract aqueous solution and petroleum ether as 1:2, place in a 2000ml separatory funnel, shake well , place...

Embodiment 2

[0030] Embodiment 2 The preparation method of whitening and ethyl acetate extract of the present invention

[0031] Accurately weigh 100g of the coarse powder of white and medicinal materials, put it into a 100ml round bottom flask, first add solvent petroleum ether to make it soaked in the medicinal surface 1cm to 2cm high, ultrasonically extract for 30min, extract twice, collect the petroleum ether extract, and extract Use a rotary evaporator to concentrate under reduced pressure at about 50°C to form an extract, use an oil bath to fully evaporate the residual organic solvent in the prepared extract, then vacuum dry the extract to obtain the extract, weigh , and calculate the extraction rate. After the petroleum ether part is extracted, respectively follow the order of chloroform, ethyl acetate, n-butanol and water to ultrasonically extract the extracts from the traditional Chinese medicine solvent parts of the corresponding solvents to obtain the petroleum ether, chloroform...

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Abstract

The invention provides a bletilla striata ethyl acetate extract which is prepared by extracting crude bletilla striata powder or an ethanol extract with petroleum ether, extracting corresponding solvent part extracts with trichloromethane, ethyl acetate, n-butyl alcohol and water in sequence, and by collecting an ethyl acetate extract part. The invention further provides application of the bletilla striata ethyl acetate extract in preparing medicines for inhibiting mycobacterium tuberculosis. The minimum bactericidal concentrations of bletilla striata ethyl acetate solvent parts extracted and prepared by using two system solvent methods are respectively 1.25 mg / ml and 0.63 mg / ml, and tests show that the MIC (Minimal Inhibitory Concentration) value of the bletilla striata ethyl acetate solvent part extract is 200 [mu]g / ml, and the MBC (Minimum Bactericidal Concentration) value is 500 [mu]g / ml, and the extract has a relatively good inhibition function on a standard strain of mycobacterium tuberculosis H37RV.

Description

technical field [0001] The invention relates to a white and ethyl acetate extract, which belongs to the field of medicine. Background technique [0002] Tuberculosis is a chronic, wasting infectious disease caused by Mycobacterium tuberculosis. Together with AIDS and malaria, tuberculosis remains a major threat to human health, especially in developing countries. According to the report of the World Health Organization, about 1 / 3 of the world's population has been infected with Mycobacterium tuberculosis, among which there are about 20 million tuberculosis patients, 8.7 million new tuberculosis patients are diagnosed every year, and about 3 million people die from the disease every year, surpassing AIDS and malaria , diarrhea and other infectious diseases, the death rate is the highest among diseases caused by single bacterial pathogen infection in the world, and tuberculosis has become one of the main causes of death from infectious diseases in adults all over the world. ...

Claims

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Application Information

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IPC IPC(8): A61K36/898A61P31/06
Inventor 严伟黎霞张宏李艾妮冉然
Owner SICHUAN NORMAL UNIVERSITY