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Method for catalyzing esterification deacidification of soybean oil by magnetic immobilized lipase

A technology of lipase catalyzing soybean and soybean oil, which is used in fatty acid esterification, fatty acid production, fatty acid chemical modification, etc.

Inactive Publication Date: 2017-07-21
NORTHEAST AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to solve various problems caused by the existing chemical deacidification and physical deacidification, the present invention proposes a method of adding sterol enzymatic esterification deacidification

Method used

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Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach 1

[0006] Specific embodiment one: step one: take the NaH that pH is 7 2 PO 4 -Na 2 HPO 4 Solution 20mL, add 10mg Candida antarctica lipase B and stir evenly, then add 100mg of the prepared active carrier NH 2 - Add SBA-15 to the previously mixed enzyme solution, add glutaraldehyde solution with a concentration of 8%, and act at 40°C for 8 hours. After the reaction is completed, collect the solid enzyme, and finally use NaH with a pH of 7 2 PO 4 -Na 2 HPO 4 Wash three times. Step 2: Using soybean oil as a substrate, the amount of magnetically immobilized lipase added is 1.5% (w / w), the mass fraction is 10% phytosterol, the reaction time is 15, 25, 35, 45, 55h, and the reaction The temperature is 60, 70, 80, 90, 100°C, and the stirring speed is 0, 50, 100, 150, 200rpm.

specific Embodiment approach 2

[0007] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the reaction time in Step 1 is 25, 35, 45 hours, and other steps are the same as Embodiment 1.

specific Embodiment approach 3

[0008] Embodiment 3: The difference between this embodiment and Embodiment 1 is that the reaction temperature in Step 1 is 70, 80, 90° C., and other steps are the same as Embodiment 1.

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PUM

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Abstract

The invention provides a method for catalyzing esterification deacidification of soybean oil by magnetic immobilized lipase. Sterol is commonly present in a crystalline form and insoluble in water, and has a limited solubility in an oil phase, but a converted product of the sterol, namely sterol ester, greatly improves the fat solubility of the sterol. Lipase is used for enzymatic esterification deacidification, but the structure of the free enzyme in an unfavorable system can change, so that the denaturation is generated to cause loss of activity, the reuse can not be realized, and further a large-scale application requirement can not be met. An enzyme immobilized on a carrier has better stability than free enzymes, the efficiency of enzyme purification and recycling can be improved, and the catalytic process is relatively easy to control. The lipase is magnetically immobilized, the sterol is added to the soybean oil, and the sterol and the free fatty acid are subjected to enzymatic esterification deacidification under the action of the magnetic immobilized lipase to reduce the soybean oil acid value to 1.550mg KOH / g, while the environmental pollution is avoided.

Description

technical field [0001] The invention relates to a method for refining soybean oil, in particular to a method for catalyzing soybean oil esterification and deacidification with magnetically immobilized lipase. Background technique [0002] The process of deep processing soybean crude oil through various physical and chemical means and biological methods is called oil refining. In soybean oil, there will be some free fatty acids (FFA), and the process of removing it is called deacidification. Alkali refining deacidification, because the use of alkali will cause the loss of neutral oil, and the acidification treatment of soapstock will also pollute the environment. Physical deacidification, due to the high temperature, will lead to the occurrence of oil side reactions and is not suitable for heat-sensitive oils, and physical deacidification requires high pre-treatment of oils, otherwise the oil obtained after refining will be dark in color, and it is difficult to further decol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B3/00C11C3/00C12N11/14
CPCC11B3/003C11C3/003C12N9/20C12N11/14C12Y301/01003
Inventor 江连洲于殿宇张如春王立琦刘天一
Owner NORTHEAST AGRICULTURAL UNIVERSITY