Primer and reagent kit of differentiating virulent and attenuated Newcastle disease viruses and differential detection method

Abstract

The invention belongs to the technical field of animal pathogeny detection and particularly relates to primer and a reagent kit of differentiating virulent and attenuated Newcastle disease viruses, and a differential detection method. The differential detection method particularly comprises the steps of extracting RNA (ribonucleic acid) of strains to be detected, dissolving RNA with 30 microliter DECP (diethyl chlorophosphite) water, performing RT-PCR (reverse transcription-polymerase chain reaction), and conducting agarose gel electrophoresis test on a DNA (deoxyribonucleic acid) amplification result. The primer designed according to different nucleotide sequences of F gene cracking sites of the virulent and attenuated NDV (Newcastle disease virus) strains has good specificity and can accurately differentiate the virulent and attenuated Newcastle disease virus strains; the reagent kit is high in sensitivity, short in consumed time, and accurate and effective in detection, can detect to-be-detected samples, with nucleic acid not less than 100 ng / microliter, of the Newcastle disease viruses and facilitates quick clinical differential diagnosis.

Description

technical field [0001] The invention belongs to the technical field of animal pathogen detection, and in particular relates to a primer for distinguishing the intensity of Newcastle disease virus, a kit thereof, and a method for identification and detection. Background technique [0002] Newcastle disease (ND), commonly known as chicken plague, is a highly contagious acute infectious disease caused by Newcastle disease virus (NDV). It is characterized by enteritis, severe diarrhea and neurological symptoms. It is one of the most important diseases, causing great economic losses. At present, this disease is prevalent in many countries and regions in the world. The World Organization for Animal Health (OIE) lists it as a statutory reportable disease (listed as a class A disease before 2005), and my country lists it as a first-class animal disease. It is a priority disease species determined in the "National Medium and Long-term Animal Disease Prevention and Control Plan (2012-...

AI Technical Summary

Problems solved by technology

[0004] With the widespread application of attenuated Newcastle disease vaccine in clinical practice, the detection of NDV alone cannot meet the needs of actual production. It is necessary to establish a detection technology to quickly distinguish between strong and attenuated vaccine strains in order to take appropriate clinical measures. preventive measures

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