A method for rapidly preparing high-purity hydroxy-α-sanshool
A technology of sanshool and hydroxyl, applied in the field of rapid preparation of high-purity hydroxy-α-sanshool, can solve the problems of long time, unstable results, poor repeatability, etc., and achieve the effects of improving purity, simple operation and low cost
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Embodiment 1
[0024] (1) Take 30 mL of red prickly ash oil, add methanol at a volume ratio of 1:20, use a constant temperature oscillator at 40°C for 1 hour, rotate at 160 rpm, and then sonicate for 5 minutes, transfer the extract into a centrifuge tube and centrifuge at 4000 r / min Take the supernatant after 15 min;
[0025] (2) Extract the supernatant with n-hexane, the ratio of solid to liquid is 1:2, extract 3 times, pass the extracted subnatant through an alkaline aluminum oxide column, and pass the obtained chromatographic solution through a 0.22 μm organic filter membrane , and finally the filtrate was evaporated to dryness under reduced pressure at 45°C (vacuum degree 0.09Mpa) with a rotary evaporator for 2 hours to obtain a crude extract;
[0026] (3) Dissolve 10mg of the crude extract in 20mL of chromatographic grade methanol, put it into a serum bottle, and irradiate it with ultraviolet light. The time is 2 hours. After the irradiation is over, use a rotary evaporator at 45°C (va...
Embodiment 2
[0028] (1) Take 30 mL of red prickly ash oil, add methanol at a volume ratio of 1:20, use a constant temperature oscillator at 40°C for 1 hour, rotate at 160 rpm, and then sonicate for 5 minutes, transfer the extract into a centrifuge tube and centrifuge at 4000 r / min Take the supernatant after 15 min;
[0029] (2) Extract the supernatant with n-hexane, the ratio of solid to liquid is 1:2, extract 3 times, pass the extracted subnatant through an alkaline aluminum oxide column, and pass the obtained chromatographic solution through a 0.22 μm organic filter membrane , and finally the filtrate was evaporated to dryness under reduced pressure at 45°C (vacuum degree 0.09Mpa) with a rotary evaporator for 2 hours to obtain a crude extract;
[0030] (3) Dissolve 10mg of the crude extract in 20mL of chromatographic grade methanol, put it into a serum bottle, and irradiate it with ultraviolet light. The time is 4 hours. After the irradiation is over, use a rotary evaporator at 45°C (va...
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