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Modification of photocrosslinked silk fibroin and preparation method of in situ drug-loaded hydrogel

A technology of silk fibroin and photocrosslinking, applied in medical science, bandages, etc., can solve problems affecting application, longer time or higher temperature, and inability to meet the low-temperature preparation requirements of drug-loaded silk fibroin composite hydrogel , to achieve the effects of controlling crosslinking density, improving physical and mechanical properties, and good biodegradability

Active Publication Date: 2019-04-19
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the chemical cross-linking method can improve the structural stability and physical and mechanical properties of the composite hydrogel, the chemical cross-linking process often requires a long time or high temperature, which cannot meet the low-temperature preparation requirements of the drug-loaded silk fibroin composite hydrogel.
In addition, commonly used chemical cross-linking agents such as aldehydes usually have certain cytotoxicity, which affects their application in the field of biomedicine

Method used

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  • Modification of photocrosslinked silk fibroin and preparation method of in situ drug-loaded hydrogel
  • Modification of photocrosslinked silk fibroin and preparation method of in situ drug-loaded hydrogel
  • Modification of photocrosslinked silk fibroin and preparation method of in situ drug-loaded hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] (1) Preparation of photocrosslinked modified silk fibroin:

[0031] According to the molar ratio of 1:1, isophorone diisocyanate and hydroxyethyl acrylate were mixed and stirred evenly at room temperature, and then reacted at 60°C for 1.5h to obtain a photocrosslinked silk fibroin modifier. Add 4% N-methylpyrrolidone dispersion of silk fibroin to it, stir and react at 80°C for 2 hours to obtain photocrosslinked modified silk fibroin, and set aside;

[0032] (2) Preparation of photocurable drug-loaded hydrogel precursor:

[0033] Table 1 The formulation of photocurable drug-loaded hydrogel precursor

[0034]

[0035] Accurately weigh each drug according to the formula in Table 1. In 2.0g deionized water, add 0.01g camphorquinone, 0.01g ethyl 4-dimethylaminobenzoate, 0.0050g polyethylene glycol diacrylate 1000, 2.0g sodium acrylate and 0.02mg recombinant human epidermal growth factor, Ultrasonic dispersion is uniform, ready for use.

[0036] (3) Preparation of phot...

Embodiment 2

[0039] The difference from Example 1 is that the types of diisocyanates are different, and other conditions are the same as in Example 1. The specific differences are as follows:

[0040] (1) Preparation of photocrosslinkable modified silk fibroin:

[0041] According to the molar ratio of 1:1, slowly drop hydroxyethyl acrylate into 1,6-hexamethylene diisocyanate, and then react at 60°C for 1.5h. Add 4% N-methylpyrrolidone dispersion of silk fibroin, stir and react at 80° C. for 2 hours to prepare photocrosslinked modified silk fibroin.

Embodiment 3

[0043] The difference from Example 1 lies in that the acrylate containing hydroxyl group is different, and the other conditions are the same as in Example 1. The specific differences are as follows:

[0044] (1) Preparation of photocrosslinked modified silk fibroin:

[0045] According to the molar ratio of 1:1, mix and stir isophorone diisocyanate and hydroxypropyl acrylate evenly, and react at 60°C for 1.5h. Then add 4% N-methylpyrrolidone dispersion of silk fibroin. Stirring and reacting at 80° C. for 2 hours to prepare photocrosslinked silk fibroin.

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Abstract

The invention discloses a modification method of a photocrosslinked silk fibroin and a preparation method of in-situ drug-loaded hydrogel of the photocrosslinked silk fibroin. The method comprises carrying out photocrosslinking modification on silk fibroin through a silane coupling agent-type modifier or a silk fibroin modifier, uniformly mixing the modified silk fibroin, a photoinitiator, a photo-curing monomer, a crosslinking agent and a drug, and preparing drug-loaded silk fibroin composite hydrogel in situ through specific light source irradiation. The method can realize covalent crosslinking of the silk fibroin, monomer and crosslinking agent in a low temperature environment, significantly improve the physical and mechanical properties of the silk fibroin composite hydrogel and adjust and control crosslinking density and biodegradability of the silk fibroin composite hydrogel system through selection of a use amount and types of the modifier, and design types and amounts of added drugs according to disease difference of patients. The photocrosslinked silk fibroin composite hydrogel has a wide application prospect in the field of tissue engineering and biomedicines.

Description

technical field [0001] The invention relates to a photocrosslinked composite hydrogel material, in particular to a photocrosslinked silk fibroin modification technology and a method for preparing the in-situ drug-loaded composite hydrogel. Background technique [0002] Hydrogel is a kind of polymer material with a three-dimensional network structure. It has excellent properties such as swelling, thixotropy, and permeability. As a wound dressing, it has the advantages of accelerated healing, easy replacement, drug loading, and sustained release. It has a broad market prospect in the medical and health field. Although synthetic polymer hydrogels have many advantages, such as stable structure, excellent performance, large yield, and low cost, they often have biocompatibility problems. Silk Fibroin (SF) is rich in 18 kinds of amino acids that are beneficial to the human body. It has excellent biocompatibility, biodegradability and drug sustained release function. It can be comp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08F289/00C08F290/06C08F220/06C08F2/48C08H1/00A61L26/00
CPCA61L26/0047A61L26/0066A61L26/008A61L26/009A61L2300/414A61L2300/604C08F2/48C08F289/00C08F290/062C08H1/00C08L89/00C08F220/06
Inventor 黄益邵建中范钦国孙广东孟一丁许晶莹
Owner ZHEJIANG SCI-TECH UNIV
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