Gold nanomaterial synthesized by dithiothreitol, preparation method and application

A technology of dithiothreitol and gold nanomaterials is applied in the field of gold nanomaterial preparation, which can solve the problems of low transfection efficiency of non-viral vectors, and achieve the effects of excellent biocompatibility, convenient synthesis and superior fluorescence performance.

Inactive Publication Date: 2017-07-28
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention provides a gold nanomaterial synthesized by using dithiothreitol, its preparation method and application, and it is the first time to prepare a highly biocompatible DTT nano-gold cluster, which solves the problem of low transfection efficiency of existing non-viral vectors

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  • Gold nanomaterial synthesized by dithiothreitol, preparation method and application
  • Gold nanomaterial synthesized by dithiothreitol, preparation method and application
  • Gold nanomaterial synthesized by dithiothreitol, preparation method and application

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Embodiment 1

[0029] A kind of gold nano material that utilizes dithiothreitol to synthesize comprises the following steps:

[0030] Step 1, mix 30mL dithiothreitol and 10mL chloroauric acid evenly, and dissolve in 100mL ultrapure water to obtain a raw material mixture solution;

[0031] Step 2, placing the raw material mixture solution at room temperature and stirring for 20 minutes to obtain a nanoparticle dispersion;

[0032] In step 3, the nanoparticle dispersion in step 2 is dialyzed with a dialysis bag with a molecular weight cut-off of 3500Da for 24 hours, and then the solution in the dialysis bag is vacuum freeze-dried to obtain a gold nanomaterial synthesized by dithiothreitol. The material is in a powder state; the vacuum freeze-drying temperature is -20-50° C., and the vacuum degree is 5-10 Pa.

Embodiment 2

[0034] A kind of gold nano material that utilizes dithiothreitol to synthesize comprises the following steps:

[0035] Step 1, mix 300mL dithiothreitol and 100mL chloroauric acid evenly, and dissolve in 1000mL ultrapure water to obtain a raw material mixture solution;

[0036] Step 2, placing the raw material mixture solution at room temperature and stirring for 10 minutes to obtain a nanoparticle dispersion;

[0037] In step 3, the nanoparticle dispersion in step 2 is dialyzed with a dialysis bag with a molecular weight cut-off of 3500Da for 24 hours, and then the solution in the dialysis bag is vacuum freeze-dried to obtain a gold nanomaterial synthesized by dithiothreitol. The material is in a powder state; the vacuum freeze-drying temperature is -20-50° C., and the vacuum degree is 5-10 Pa.

Embodiment 3

[0039] A kind of gold nano material that utilizes dithiothreitol to synthesize comprises the following steps:

[0040] Step 1, mix 30mL dithiothreitol and 10mL chloroauric acid evenly, and dissolve in 200mL ultrapure water to obtain a raw material mixture solution;

[0041] Step 2, placing the raw material mixture solution at room temperature and stirring for 30 minutes to obtain a nanoparticle dispersion;

[0042] In step 3, the nanoparticle dispersion in step 2 is dialyzed with a dialysis bag with a molecular weight cut-off of 3500Da for 24 hours, and then the solution in the dialysis bag is vacuum freeze-dried to obtain a gold nanomaterial synthesized by dithiothreitol. The material is in a powder state; the vacuum freeze-drying temperature is -20-50° C., and the vacuum degree is 5-10 Pa.

[0043] In order to observe the dispersibility of the gold nanomaterial synthesized by dithiothreitol of the present invention in water, we placed the solution in the dialysis bag obtain...

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Abstract

The invention discloses a preparation method of a gold nanomaterial synthesized by dithiothreitol. The preparation method comprises the following steps: dithiothreitol and chloroauric acid are uniformly mixed according to a volume ratio of 3: 1, and are dissolved in ultrapure water to obtain raw material mixture solution; the raw material mixture solution is stirred by 10-30 min at room temperature to obtain nanoparticle dispersion liquid; the nanoparticle dispersion liquid in the step (2) is dialyzed by 24 h in a dialyzing bag; and solution in the dialyzing bag is frozen and dried in vacuum to obtain the gold nanomaterial synthesized by dithiothreitol. The gold nanomaterial is convenient to synthesize, excellent and stable in fluorescence performance and excellent in biologic compatibility; and a gold cluster is applied, so that users can wrap external source genes and lead into target cells to finish the gene treatment purpose.

Description

technical field [0001] The invention belongs to the technical field of gold nanomaterial preparation, and in particular relates to a gold nanomaterial synthesized by using dithiothreitol, a preparation method and an application. Background technique [0002] In recent years, fluorescent labeling has become a very important research tool in the fields of biological science and clinical medicine. Currently, fluorescent labeling materials usually include noble metal nanoclusters, quantum dots, composite fluorescent nanoparticles, and organic dyes. Because quantum dots and organic small molecule dyes are generally more toxic to organisms, and the synthesis cost of noble metal nanoclusters is not low, the advantages of composite fluorescent nanomaterials are reflected. [0003] Generally speaking, composite fluorescent nanomaterials have the characteristics of simple synthesis method, low preparation cost, good biocompatibility and stable fluorescence performance, and have attra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B22F9/24A61K49/00A61K48/00B82Y40/00
CPCA61K48/0033A61K49/0065B22F9/24B82Y40/00
Inventor 陈志俊荆江博丁晗
Owner JILIN UNIV
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