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Application of glutathione-templated gold nanoclusters in the detection of cysteine ​​and lysine

A gold nanocluster and glutathione technology, applied in the field of amino acid detection, can solve the problems of complex operation, long reaction time, low sensitivity and selectivity, and achieve the effect of simple preparation method and rapid detection

Active Publication Date: 2020-11-10
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The detection of cysteine ​​and lysine is usually affected by long reaction time, reaction conditions such as pH acidity and alkalinity, complex operation, low sensitivity and selectivity

Method used

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  • Application of glutathione-templated gold nanoclusters in the detection of cysteine ​​and lysine
  • Application of glutathione-templated gold nanoclusters in the detection of cysteine ​​and lysine
  • Application of glutathione-templated gold nanoclusters in the detection of cysteine ​​and lysine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Preparation and characterization of glutathione-stabilized gold nanoclusters

[0041] 1. Preparation of gold nanoclusters (GSH-Au NCs) using glutathione as a template

[0042] Synthesis of gold nanoclusters with fluorescent properties using glutathione as a template: The synthesis of fluorescent gold nanoclusters was based on the method reported in the literature [see: Chen W. B., Tu X. J., Guo X. Q. Chem. Commun., 2009, 1736–1738.], made some improvements, the process is as follows: First, the newly prepared glutathione aqueous solution (5 mM, 10 mL) and tetrachloroauric acid aqueous solution (5 Mm, 10 mL) were vigorously stirred The mixture was mixed down, and then the mixture was reacted for 2 to 5 days to obtain the product as a light yellow solution. In this experiment, the concentration ratio of reactants and raw materials is 1:1, and the reaction conditions are: reaction temperature 30°C, and reaction environment is sunlight or sunlight lighting cond...

Embodiment 2

[0047] Example 2 Glutathione-stabilized gold nanocluster fluorescent probe for detection of cysteine

[0048] This example will describe in detail how to use the gold nanoclusters of the glutathione template prepared in Example 1 to detect cysteine.

[0049] The specific steps for the detection of cysteine ​​using the prepared fluorescent glutathione-stabilized gold nanocluster probe: Take 300 μL of the prepared glutathione-stabilized gold nanocluster sample, add it to a centrifuge tube, and then add a series of concentration of cysteine ​​working solution, respectively detect the effects of different concentrations of cysteine ​​on the fluorescence signal of glutathione-stabilized gold nanocluster probes (final concentrations are 0, 5, 10, 15, 20, 25, 50, 75, 100, 125, 150, 175, 200, 225, 250, 300 µM). Dilute with double distilled water to a final volume of 3 mL, mix well, and measure. Under the excitation of 402 nm wavelength light, the fluorescence spectrum of glutathione...

Embodiment 3

[0051] Example 3 Glutathione-stabilized gold nanocluster ratiometric fluorescent probe for detection of lysine

[0052] This example will describe in detail how to use the gold nanoclusters of glutathione template prepared in Example 1 to detect lysine.

[0053] Specific steps for detection of lysine using the prepared glutathione-stabilized gold nanocluster ratiometric fluorescent probe: Take 300 μL of the prepared glutathione-stabilized gold nanocluster sample, add it to a centrifuge tube, and then add a series of concentration of lysine working solution, the effects of different concentrations of lysine on the fluorescence signal of glutathione-stabilized gold nanocluster probes were detected respectively (final concentrations were 0, 2.5, 5, 7.5, 10, 12.5, 15, 17.5, 20, 22.5, 25 µM). Dilute with double distilled water to a final volume of 3 mL, mix well, and measure. Under the excitation of 402 nm wavelength light, the fluorescence spectrum of glutathione-stabilized gold...

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Abstract

The invention discloses application of glutathione-stabilized gold nano-clusters in detection of cysteine and lysine contents. The application comprises the following steps: mixing a newly prepared glutathione aqueous solution and a chloroauric acid hydrated aqueous solution under the vigorous stirring in equal concentration and equal volume, and reacting an obtained mixture for 2-5 days at an illumination condition to obtain a light yellow solution, centrifuging, purifying supernate through a dialyser so as to prepare the glutathione-stabilized gold nano-clusters. The glutathione-stabilized gold nano-clusters can be used as a fluorescent probe or a ratiometric fluorescent probe to respectively or simultaneously detect cysteine and lysine. The application of the glutathione-stabilized gold nano-clusters in the detection of the cysteine and the lysine contents has the characteristics of being high in selectivity, high in sensitivity, simple and easy to implement, and the preparation method is simple and convenient, green and gentle.

Description

technical field [0001] The invention belongs to the field of amino acid detection, and in particular relates to the application of glutathione-stabilized gold nanoclusters in detecting the contents of cysteine ​​and lysine. Background technique [0002] Amino acids are the basic constituent units of biological functional macromolecular proteins. The 20 kinds of amino acids in the human body have different functions, promote and influence each other. If the human body lacks any essential amino acid, it can lead to abnormal physiological functions, affect the normal progress of antibody metabolism, and eventually lead to diseases. Diseases caused by amino acid deficiency and metabolic disorders involve various diseases such as immunity, nervous system, cardiovascular and cerebrovascular diseases, kidney, metabolism, diabetes, etc., affecting human growth and development, nutritional health, musculoskeletal growth, hormone secretion, detoxification function and other health li...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/6402G01N21/6486G01N2021/6419
Inventor 冯大千刘国良王俊琦张静茹尹倩茹
Owner YANCHENG INST OF TECH
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