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Method for quantitatively detecting abiraterone in whole blood

A quantitative detection method, abiraterone technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems that it is difficult to find out the content of abiraterone in whole blood, and the content of abiraterone has not been known, and achieve the improvement of sensitivity , Strong elution ability, fast elution effect

Active Publication Date: 2017-08-04
CHINA JILIANG UNIV
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Problems solved by technology

However, this study only quantitatively analyzed the content of abiraterone in animal plasma. Animal plasma is a type of biological sample that removes cellular components such as red blood cells, white blood cells, and lymphocytes. already relatively clean
Therefore, the current research only quantitatively detects the content of abiraterone in plasma or serum, and has not yet known the content of abiraterone in various blood cells (including red blood cells, white blood cells, etc.) contained in blood, and it is difficult to calculate the content of abiraterone in whole blood Bitterone content

Method used

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  • Method for quantitatively detecting abiraterone in whole blood
  • Method for quantitatively detecting abiraterone in whole blood
  • Method for quantitatively detecting abiraterone in whole blood

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Embodiment 1

[0047] Embodiment 1, a kind of abiraterone pretreatment method and quantitative detection means in animal (human) whole blood, carry out following steps successively:

[0048] 1) Put 0.2mL of venous whole blood separated from the animal body / human body into a heparinized centrifuge tube, add 0.2mL of cell lysate, and shake (shake at a frequency of 100r / min for 5 minutes) to completely lyse the blood cells; Whole blood lysate.

[0049] The formula of the above-mentioned cell lysate is: 8.29g of ammonium chloride, 1g of potassium bicarbonate and 37.2mg of EDTA-2Na, which are dissolved in 1000mL of ultrapure water and stirred to dissolve.

[0050] Accurately draw 0.4 mL of whole blood lysate into a centrifuge tube with a cover. After shaking and mixing, add 3 mL of ethyl acetate to it accurately, vortex (100 r / min frequency) for 2 min, and centrifuge at 8000 r / min for 5 min to obtain Ethyl acetate layer in the upper layer, residue in the lower layer. Take out all the ethyl acet...

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Abstract

The invention discloses a method for quantitatively detecting abiraterone in whole blood. The method adopts whole blood as an object for detecting, and sequentially comprises the following steps: adding ethyl acetate to whole blood lysate, performing vortex shaking, and centrifuging; drying the ethyl acetate layer with nitrogen airflow by means of blowing, redissolving with 20% acetonitrile aqueous solution, centrifuging, and extracting supernatant I; weighing alkaline diatomite and putting into a glass chromatographic column, vibrating, adding the supernatant I, eluting with pure water, 20% methanol aqueous solution and chromatographic methanol sequentially, drying the eluent with nitrogen airflow by means of blowing, redissolving with 20% acetonitrile aqueous solution, centrifuging, extracting supernatant II, and analyzing with a high performance liquid chromatography system to obtain an abiraterone drug peak area Y, putting into equation Y equaling to 27524X plus 1956.6, wherein X is the abiraterone concentration of the supernatant II; and performing conversion to obtain the abiraterone concentration in the whole blood for detecting.

Description

technical field [0001] The invention relates to a quantitative detection method for abiraterone in animal (including human) whole blood. Background technique [0002] Prostate cancer is a common malignant tumor in European and American men, with about 240,000 new diagnoses every year, and it is the second most common cancer in male cancer mortality, and it is highly metastatic. According to statistics, about 28,000 people died of the disease in 2012. Although the incidence of prostate cancer in my country is much lower than that in Western countries, with the change of lifestyle and the popularization of prostate cancer-specific antigen screening, the number of patients with prostate cancer in my country has also shown a linear upward trend, and most of them develop into advanced stages prostate cancer. The main mechanism of its development is that intracellular androgen receptors are continuously stimulated by androgen. At present, the preferred treatment method for prosta...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
Inventor 邓同乐葛建林芳黄丽红周益峰胡华军张永勇刘冠男
Owner CHINA JILIANG UNIV
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