Method for simultaneously determining four components in semen ziziphi spinosae by adopting accelerated solvent extraction-charged aerosol detector

A technology of electric aerosol detector and wild jujube kernel, which is applied in the field of chemical detection, can solve the problems of high instrument price, complicated operation, and difficult maintenance, and achieve the effect of good linear relationship and improved extraction efficiency

Inactive Publication Date: 2017-08-04
WUZHOU INST FOR FOOD & DRUG CONTROL
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Problems solved by technology

For the determination of active ingredients in jujube seed, traditional methods mostly use liquid phase diode array detectors, evaporative light detectors and liquid phase mass spectrometry detection systems. [15-16] However, jujube saponins A, B and betulinic acid are triterpenoid saponins, and their ultraviolet absorption is poor. The sensitivi

Method used

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  • Method for simultaneously determining four components in semen ziziphi spinosae by adopting accelerated solvent extraction-charged aerosol detector
  • Method for simultaneously determining four components in semen ziziphi spinosae by adopting accelerated solvent extraction-charged aerosol detector
  • Method for simultaneously determining four components in semen ziziphi spinosae by adopting accelerated solvent extraction-charged aerosol detector

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Embodiment 1

[0019] 1 Instruments and reagents

[0020] 1.1 Instrument

[0021] ThermoFisher SCITIFIC ULTIMATE 3000 high performance liquid chromatograph is equipped with charged aerosol detector Corona Ultra (Thermo Fisher Scientific), ASE350 fast solvent extraction instrument (Thermo Fisher Scientific), XA205DU electronic analytical balance (Mettler Instrument Co., Ltd.), ultrapure water machine (Merck Millipore), EV311-V rotary evaporator (Beijing Labtech Technology Co., Ltd.).

[0022] 1.2 Reagent

[0023] Reference products jujuboside A (batch number 110734-201512, content is 95.6%), jujuboside B (batch number 110814-200506, content is 100%), spinosin (batch number 111869-201203, content is 95.4%) ) and betulinic acid (batch number 111802-201001, with a content of 100%) were provided by the China Institute for Food and Drug Control; acetonitrile was of chromatographic grade, and the rest of the reagents were of analytical grade.

[0024] Suanzaoren medicinal materials are all comme...

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Abstract

The invention discloses a method for simultaneously determining four components in semen ziziphi spinosae by adopting an accelerated solvent extraction-charged aerosol detector and aims at providing a method for simultaneously determining the four components in the semen ziziphi spinosae by adopting the accelerated solvent extraction-charged aerosol detector which is efficient, accurate and good in reproducibility. According to the technical main points, the method comprises the steps of (1) preparing a reference substance stock solution; (2) preparing a test sample solution; and (3) injecting a reference substance solution and the test sample solution into chromatograph for detection separately, wherein a chromatographic column is Thermo Synronis C18, a mobile phase is acetonitrile-water, the gradient elution program is 10-60% of acetonitrile and 90-40% of water for 0-8min, 60-80% of acetonitrile and 40-20% of water for 8-10, 80-100% of acetonitrile and 20-0% of water or 10-13min, and 100% of acetonitrile for 13-16min, the flow rate is 0.5mL.min<-1>, the column temperature is 40 DEG C, the atomization temperature is 35 DEG C and the sample size is 1muL. The method belongs to the technical field of chemical detection.

Description

technical field [0001] The invention relates to a method for simultaneously determining the contents of multiple components in Ziziphus jujube kernels, in particular to a method for simultaneously measuring four kinds of ingredients in Zizinus jujube kernels by rapid solvent extraction and an electric aerosol detector, and belongs to the technical field of chemical detection. Background technique [0002] Ziziphus jujuba Mill.var.Spinosa (Bunge) Huex H.F.Chou is the dry mature seed of Ziziphus jujuba Mill.var.Spinosa (Bunge) Huex H.F.Chou. It has the effects of nourishing the heart and nourishing the liver, calming the mind and calming the nerves, suppressing sweat, and promoting fluid production. It is often used for insomnia, palpitations and dreaminess, body deficiency and hyperhidrosis, and thirsty wounds. Its main effective chemical components are triterpenoid saponins, flavonoids, triterpenoids, alkaloids, etc. It has sedative and hypnotic, anti-anxiety, anti-convulsa...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 陈学松罗达龙王丽丽刘慧妍黄馨慧
Owner WUZHOU INST FOR FOOD & DRUG CONTROL
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