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Extraction method for hyperglycemic longan seed polyphenol

An extraction method, the technology of longan kernel, which is applied in the field of polyphenol extraction from longan kernel for lowering blood sugar, can solve the problems of limited scale, complex process, equipment investment, high production cost, etc.

Inactive Publication Date: 2017-08-08
GUANGXI NANYIZAI SCI & TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at the present stage, there is no report of a process method with practical production significance for the extraction and purification process of longan kernel polyphenols. The scale is still only in the laboratory stage, and the equipment investment and production cost are high, the process is complex, the introduction of additives and impurities is more, and the purity and activity of the finished product need to be further improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] (1) Wash the longan core, and dry it for 15 hours at a temperature of 45° C., so that the final water loss rate of the longan core reaches 25%;

[0034] (2) pulverize the dried longan core into 30 orders, and obtain longan core powder;

[0035] (3) Add water to adjust the slurry according to the material-water ratio of 1:4. After stirring evenly, add 1200U α-amylase per gram of the material-water mixture, and liquefy at pH 5.5 and temperature 90°C for 30 minutes to obtain the primary enzymatic hydrolyzate;

[0036] (4) adding cellulase to the primary hydrolyzate for enzymolysis, adding 1200U cellulase according to 0.3% of the primary hydrolyzate, and enzymolyzing at pH 4.5 and temperature 40°C for 3 hours to obtain the hydrolyzate;

[0037] (5) Add 20% sodium sulfate solution to the enzymatic hydrolyzate, stir evenly and set the microwave power to 600W, 700W, 800W, 700W, 600W, 700W, 800W at a microwave frequency of 3300MHZ every 10s.

[0038] (6) Filtering and drying t...

Embodiment 2

[0042] (1) Wash the longan core and dry it for 30 hours at a temperature of 55° C., so that the final water loss rate of the longan core reaches 32%;

[0043] (2) pulverize the dried longan core into 40 orders to obtain longan core powder;

[0044] (3) Add water to adjust the slurry according to the ratio of material to water 1:4. After stirring evenly, add 1200U α-amylase per gram of the material-water mixture, and liquefy at pH 5.5 and temperature 90°C for 60 minutes to obtain the primary enzymatic hydrolyzate;

[0045] (4) adding cellulase to the primary hydrolyzate for enzymolysis, adding 1200U cellulase according to 0.3% of the primary hydrolyzate, and enzymolyzing at pH 4.5 and temperature 40°C for 3 hours to obtain the hydrolyzate;

[0046] (5) Add 40% sodium sulfate solution to the enzymatic hydrolyzate, stir evenly, and set the microwave power to 700W, 800W, 700W, 800W in sequence every 20s at a microwave frequency of 3300MHZ.

[0047] (6) Filtering and drying the ex...

Embodiment 3

[0051] (1) Wash the longan core and dry it for 22 hours at a temperature of 47° C., so that the final water loss rate of the longan core reaches 30%;

[0052] (2) pulverize the dried longan core into 35 orders, and obtain longan core powder;

[0053] (3) Add water to adjust the slurry according to the material-water ratio of 1:4. After stirring evenly, add 1200 Uα-amylase per gram of the material-water mixture, and liquefy at pH 5.5 and temperature 90°C for 45 minutes to obtain primary enzymatic hydrolyzate;

[0054] (4) adding cellulase to the primary hydrolyzate for enzymolysis, adding 1200U cellulase according to 0.3% of the primary hydrolyzate, and enzymolyzing at pH 4.5 and temperature 40°C for 3 hours to obtain the hydrolyzate;

[0055](5) Add 30% sodium sulfate solution to the enzymatic hydrolyzate, stir evenly and set the microwave frequency at 3300MHZ every 12s to 500W, 600W, 700W, 600W, 500W, 600W, 700W.

[0056] (6) Filtering and drying the extraction solution unde...

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Abstract

The invention relates to the technical field of polyphenol extraction, especially to an extraction method for hyperglycemic longan seed polyphenol. The method includes the steps of: (1) cleaning and drying longan seeds; (2) crushing the dried longan seeds to obtain longan seed powder; (3) adding water into the longan seed powder to make a pulp, then adding alpha-amylase for enzymolysis, thus obtaining a primary enzymolysis product; (4) adding cellulose into the primary enzymolysis product for enzymolysis, thus obtaining an enzymolysis product; (5) adding a sodium sulfate solution into the enzymolysis product, stirring the substances evenly, and then conducting microwave extraction so as to obtain leachate; (6) subjecting the leachate to filtration under reduced pressure and drying the extracted liquid, thus obtaining a longan seed polyphenol crude extract; (7) adding water into the longan seed polyphenol crude extract to prepare a solution, and letting the solution pass through macroporous resin for adsorption; and (8) filtering the adsorbed resin, using 35% ethanol to perform elution, concentrating the eluent and then performing drying, thus obtaining a polyphenol extract. The method provided by the invention can effectively extract the hyperglycemic polyphenol component from longan seeds.

Description

[0001] 【Technical field】 [0002] The invention relates to the technical field of polyphenol extraction, in particular to a method for extracting blood sugar-lowering longan kernel polyphenols. [0003] 【Background technique】 [0004] Longan, the fruit of Sapindaceae longan, is warm in nature and sweet in taste, nourishes the heart and spleen, nourishes qi and blood, and has a good nourishing and nourishing effect. China is the world's largest producer of longan. It is mainly produced in Guangdong, Guangxi, Fujian, Sichuan and other places with more than 10,000 tons of longan meat. The longan core is the seed kernel of longan, with a diameter of 0.8-1.2cm. The skin is reddish brown and shiny. There is a long oval gray-white hilum at one end, and the quality is hard. The smell is light and slightly bitter. The quality of longan core accounts for about 17% of the quality of longan fruit. Due to the lack of comprehensive utilization, the quality of discarded longan cores reache...

Claims

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Application Information

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IPC IPC(8): A61K36/77A61P3/10A61K131/00
CPCA61K36/77A61K2236/15A61K2236/17A61K2236/19A61K2236/333A61K2236/51A61K2236/53A61K2236/55
Inventor 覃永红
Owner GUANGXI NANYIZAI SCI & TECH CO LTD
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