Mallotus furetianus propagation method
The technology of partridge tea and culture medium is applied in the field of plant cultivation, which can solve the problems of the artificial scale planting of partridge tea and the lack of supporting cultivation technology, the development and utilization of partridge tea resources and other problems, so as to achieve good social and economic benefits, The effect of preventing virus infection and promoting industrialization
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Embodiment 1
[0022] A method for breeding partridge tea, comprising the steps of:
[0023] Step (1), select partridge tea stems with robust growth and axillary buds as explants, clean them and disinfect them, and put them in H medium + NAA 0.15 mg / L + potato juice 20% (V / V) + Agar 5g / L, culture medium with pH 5.8-6.0, cultivate for 3 days, replace the medium with MS medium + 6-BA 4mg / L + NAA 0.01mg / L + agar 6g / L + sucrose 30 g / L, Culture medium with a pH of 5.8-6.0, continue to cultivate for 20 days;
[0024] Step (2), cut off the buds cultivated in step (1), put them in DKW medium + brassinolide 0.15mg / L + 2mg / L paclobutrazol + coconut milk 10 g / L + sucrose 50g / L + agar 5g / L, pH 5.7-5.9 culture medium for 18 days;
[0025] Step (3), put the sprouts cultivated in step (2) in 1 / 2MS medium+IBA 1mg / L+6-BA 6mg / L+sucrose 20g / L+PVP 2g / L+activated carbon 0.8g / L, Rooting culture is carried out in a medium with a pH of 5.8-6.0;
[0026] Step (4), when the rooting culture in step (3) is cultivat...
Embodiment 2
[0030] Step (1), select partridge tea stems with robust growth and axillary buds as explants, clean and disinfect them, and put them in H medium + NAA 0.18mg / L + potato juice 20% (V / V) + Agar 5g / L, pH 5.8-6.0 culture medium, culture 5d, replace medium, replace with MS medium + 6-BA 4.5mg / L + NAA 0.03 mg / L + agar 6.5 g / L + sucrose 30 g / L, medium with a pH of 5.8-6.0, continue to cultivate for 23 days;
[0031] Step (2), cut off the buds cultivated in step (1), put them in DKW medium + brassinolide 0.17mg / L + 2.4mg / L paclobutrazol + coconut milk 11g / L + sucrose 570g / L + agar 5.2g / L, culture medium with pH 5.7-5.9 for 20 days;
[0032] Step (3), place the sprouts cultivated in step (2) in 1 / 2MS medium+IBA 1.4mg / L+6-BA 7mg / L+sucrose 22g / L+PVP 2.5 g / L+activated carbon 0.8g / L, the culture medium that pH is 5.8-6.0 carries out rooting culture;
[0033] Step (4), when the rooting culture in step (3) is cultivated until the root length is 3-3.5cm, it can be transplanted. After tra...
Embodiment 3
[0037] Step (1), select partridge tea stems with robust growth and axillary buds as explants, clean and disinfect them, and put them in H medium + NAA 0.2mg / L + potato juice 20% (V / V) + Agar 5g / L, culture medium with pH 5.8-6.0, culture for 5 days, replace the medium with MS medium + 6-BA 5mg / L + NAA 0.05 mg / L + agar 7 g / L + sucrose 30 g / L , culture medium with a pH of 5.8-6.0, and continue to cultivate for 25 days;
[0038]Step (2), cut off the buds cultivated in step (1), put them in DKW medium + brassinolide 0.2mg / L + 2.5mg / L paclobutrazol + coconut milk 15 g / L + sucrose 80g / L+ Agar 5.5g / L, pH 5.7-5.9 culture medium for 22 days;
[0039] Step (3), put the sprouts cultivated in step (2) in 1 / 2MS medium+IBA 2mg / L+6-BA 8mg / L+sucrose 25g / L+PVP 3 g / L+activated carbon 0.8g / L , and the pH is 5.8-6.0 in the medium for rooting culture;
[0040] Step (4), when the rooting culture in step (3) is cultivated until the root length is 3-3.5cm, it can be transplanted. After transplantin...
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