Mallotus furetianus propagation method

The technology of partridge tea and culture medium is applied in the field of plant cultivation, which can solve the problems of the artificial scale planting of partridge tea and the lack of supporting cultivation technology, the development and utilization of partridge tea resources and other problems, so as to achieve good social and economic benefits, The effect of preventing virus infection and promoting industrialization

Active Publication Date: 2017-08-11
尚南堂海南生态农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the development and utilization of partridge tea resources are still at the level of utilizing wild resources, and the artificial scale planting and supporting cultivation techniques of partridge tea required for industrial development are still in the blank
So far, there is no large-scale planting base for commercial production of partridge tea products in Hainan Island or at home and abroad. Almost all partridge tea products in the market are directly obtained from wild plants.

Method used

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  • Mallotus furetianus propagation method
  • Mallotus furetianus propagation method
  • Mallotus furetianus propagation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for breeding partridge tea, comprising the steps of:

[0023] Step (1), select partridge tea stems with robust growth and axillary buds as explants, clean them and disinfect them, and put them in H medium + NAA 0.15 mg / L + potato juice 20% (V / V) + Agar 5g / L, culture medium with pH 5.8-6.0, cultivate for 3 days, replace the medium with MS medium + 6-BA 4mg / L + NAA 0.01mg / L + agar 6g / L + sucrose 30 g / L, Culture medium with a pH of 5.8-6.0, continue to cultivate for 20 days;

[0024] Step (2), cut off the buds cultivated in step (1), put them in DKW medium + brassinolide 0.15mg / L + 2mg / L paclobutrazol + coconut milk 10 g / L + sucrose 50g / L + agar 5g / L, pH 5.7-5.9 culture medium for 18 days;

[0025] Step (3), put the sprouts cultivated in step (2) in 1 / 2MS medium+IBA 1mg / L+6-BA 6mg / L+sucrose 20g / L+PVP 2g / L+activated carbon 0.8g / L, Rooting culture is carried out in a medium with a pH of 5.8-6.0;

[0026] Step (4), when the rooting culture in step (3) is cultivat...

Embodiment 2

[0030] Step (1), select partridge tea stems with robust growth and axillary buds as explants, clean and disinfect them, and put them in H medium + NAA 0.18mg / L + potato juice 20% (V / V) + Agar 5g / L, pH 5.8-6.0 culture medium, culture 5d, replace medium, replace with MS medium + 6-BA 4.5mg / L + NAA 0.03 mg / L + agar 6.5 g / L + sucrose 30 g / L, medium with a pH of 5.8-6.0, continue to cultivate for 23 days;

[0031] Step (2), cut off the buds cultivated in step (1), put them in DKW medium + brassinolide 0.17mg / L + 2.4mg / L paclobutrazol + coconut milk 11g / L + sucrose 570g / L + agar 5.2g / L, culture medium with pH 5.7-5.9 for 20 days;

[0032] Step (3), place the sprouts cultivated in step (2) in 1 / 2MS medium+IBA 1.4mg / L+6-BA 7mg / L+sucrose 22g / L+PVP 2.5 g / L+activated carbon 0.8g / L, the culture medium that pH is 5.8-6.0 carries out rooting culture;

[0033] Step (4), when the rooting culture in step (3) is cultivated until the root length is 3-3.5cm, it can be transplanted. After tra...

Embodiment 3

[0037] Step (1), select partridge tea stems with robust growth and axillary buds as explants, clean and disinfect them, and put them in H medium + NAA 0.2mg / L + potato juice 20% (V / V) + Agar 5g / L, culture medium with pH 5.8-6.0, culture for 5 days, replace the medium with MS medium + 6-BA 5mg / L + NAA 0.05 mg / L + agar 7 g / L + sucrose 30 g / L , culture medium with a pH of 5.8-6.0, and continue to cultivate for 25 days;

[0038]Step (2), cut off the buds cultivated in step (1), put them in DKW medium + brassinolide 0.2mg / L + 2.5mg / L paclobutrazol + coconut milk 15 g / L + sucrose 80g / L+ Agar 5.5g / L, pH 5.7-5.9 culture medium for 22 days;

[0039] Step (3), put the sprouts cultivated in step (2) in 1 / 2MS medium+IBA 2mg / L+6-BA 8mg / L+sucrose 25g / L+PVP 3 g / L+activated carbon 0.8g / L , and the pH is 5.8-6.0 in the medium for rooting culture;

[0040] Step (4), when the rooting culture in step (3) is cultivated until the root length is 3-3.5cm, it can be transplanted. After transplantin...

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Abstract

The invention relates to a mallotus furetianus propagation method and belongs to the technical field of plant cultivation. The method comprises the steps that mallotus furetianus stem segments growing healthy and strong and provided with axillary buds serve as explants, are cleaned thoroughly and are subjected to disinfection treatment, then culture is performed in an H culture medium and an NAA, potato juice and agar culture medium and then is performed in an MS culture medium and a 6-BA, NAA, agar and sucrose culture medium, buds obtained through culture are cut off for multiplication culture, then bud seedlings obtained through multiplication culture undergo rooting culture, and transplantation can be performed when the root length is 3-3. 5 cm. The problems of seedling source shortage, low survival rate of transplantation and the like existing in artificial large-area mallotus furetianus cultivation can be solved, a key effect is played, the survival rate of transplantation is 95% or above, the yield is improved by 40% or above, industrial and large-scale mallotus furetianus development can be greatly promoted, and the mallotus furetianus propagation method has good social and economic benefits.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and in particular relates to a breeding method of partridge tea. Background technique [0002] Partridge tea, a specialty of Hainan, is a peculiar wild tea with round leaves and sweet taste. It is mainly produced in the mountains, hills and coastal areas of Wanning, Hainan, which is known as the "Hometown of Longevity in the World". The large leaves of wild partridge tea trees are of high quality and original ecology. The taste of the product is sweet and spicy, fragrant and warm, exuding a strong Lingling aroma. For thousands of years, it has been hailed as the "Ganoderma lucidum" in tea products by literati and inkmen of all dynasties. It is a green health-preserving and healthy drink that people in various places in Hainan drink in daily life, drink it frequently in four seasons and receive guests. Tian Han, a famous Chinese poet and playwright, once wrote a poem when he climbed Do...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G23/04C05G1/00
CPCA01G23/04A01H4/001A01H4/008C05D9/00C05F5/00C05F5/002C05F11/02
Inventor 于世宁
Owner 尚南堂海南生态农业科技有限公司
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