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Tissue culture rapid propagation method for high-quality Shatian pomelos

A technology of tissue culture and Shatian pomelo, which is applied in the field of plant tissue culture and can solve problems such as low efficiency and slow speed

Inactive Publication Date: 2017-08-11
玉林市林业科学研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the propagation of Shatian pomelo seedlings mainly relies on seed propagation. These propagation methods are slow and inefficient, and cannot meet the needs of high-quality seedlings for large-scale planting of fine varieties of Shatian pomelo. Therefore, it is very necessary to establish a complete set of Shatian pomelo in Rongxian County. tissue culture rapid propagation system

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1), explant pretreatment

[0017] Shatian pomelo stems with terminal buds collected from the field were washed with tap water to clean the sediment, soaked in saturated washing powder solution for 7 minutes, rinsed with running tap water, and placed in a 3°C refrigerator for 12 hours before use.

[0018] 2), training process

[0019] (1) Induction of adventitious buds: Shatian pomelo stems with terminal buds were sterilized with 75% alcohol for 1 minute, rinsed with sterile water for 3 times, then placed in 0.1% mercuric chloride solution for 15 minutes, and then rinsed with sterile water for 5 minutes. The second time, inoculated on the induction medium after blotted dry by sterile filter paper, cultured in total darkness at 28°C, and formed adventitious buds after 7 days of culture. The induction medium used is: MS+0.5mg / L 6-BA+0.3mg / L NAA+3.0mg / L riboflavin+0.1mg / L D-calcium pantothenate+1.5mg / L ascorbic acid+1.5mg / L L L-cysteine ​​+ 0.01mg / L biotin + 3.0% sucrose ...

Embodiment 2

[0023] 1), explant pretreatment

[0024] Rinse the sandy pomelo with terminal buds and side buds collected from the wild with tap water, soak in saturated washing powder solution for 10 minutes, then rinse with running tap water for 24 hours, seal it with a plastic bag, and place it in a refrigerator at 5°C spare.

[0025] 2), training process

[0026] (1) Induction of adventitious buds: Shatian pomelo with terminal buds and side bud stems were sterilized with 75% alcohol for 5 minutes, rinsed with sterile water for 5 times, placed in 0.2% mercuric chloride solution for 10 minutes, and then sterilized with sterile water Rinse 6 times, blot dry with sterile filter paper, inoculate on the induction medium, culture at 25°C in total darkness, and form adventitious buds after 10 days of cultivation. The induction medium used is: MS+1mg / L 6-BA+0.3mg / L NAA+3.0mg / L riboflavin+0.2mg / L D-calcium pantothenate+1.0mg / L ascorbic acid+1.0mg / L L-cysteine ​​+ 0.03mg / L biotin + 3.0% sucrose ...

Embodiment 3

[0030]1), explant pretreatment

[0031] Rinse the sandy pomelo with terminal buds and side buds collected from the wild with tap water, soak in saturated washing powder solution for 7 minutes, then rinse with running tap water for 8 hours, seal with a plastic bag, and place in a refrigerator at 5°C Set aside overnight.

[0032] 2), training process

[0033] (1) Induction of adventitious buds: Shatian pomelo with terminal buds and side buds were sterilized with 80% alcohol for 5 minutes, rinsed with sterile water for 4 times, then placed in 0.1% mercuric chloride solution for 14 minutes, and then sterilized with sterile water Rinse 5 times, blot dry with sterile filter paper, inoculate on induction medium, and culture in total darkness at 25°C. Adventitious buds will form after 14 days of culture. The induction medium used is: MS+0.8mg / L 6-BA+0.4mg / L NAA+3.0mg / L riboflavin+0.1mg / L D-calcium pantothenate+2.0mg / L ascorbic acid+2.0mg / L L L-cysteine ​​+ 0.03mg / L biotin + 3.0% su...

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Abstract

The invention discloses a tissue culture rapid propagation method for high-quality Shatian pomelos. The method is characterized by treating the Shatian pomelo trees in the stages of adventitious bud induction, multiple shoot multiplication, test-tube plantlet rooting, and the like, and abundant test-tube plantlets of the Shatian pomelos are acquired in the manner of rapid propagation in vitro of the multiple shoots, so that the purpose of the invention is achieved. The tissue culture rapid propagation method for high-quality Shatian pomelos is characterized by simpleness, feasibility and economic effect. The sprout guarantee can be supplied for the protection and large-scale planting of excellent varieties of Shatian pomelos.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for rapid propagation of tissue culture of high-quality Shatian pomelo. Background technique [0002] Shatian pomelo is originally produced in Rong County, Guangxi, and later developed into Fujian, Guangdong, Guangxi Guilin, Guangxi Liuzhou and other places to introduce Shatian pomelo in Guangxi Rongxian. Shatian pomelo is an excellent variety of pomelo, and Shatian pomelo is a subtropical evergreen tree. Rutaceae Citrus. Shatian pomelo is rich in nutrition, directly edible pulp accounts for 51.8 to 58.8%, soluble solids are 12 to 16%, crude protein is 6.4% (dry matter), sugar content is about 12%, and acid content is 0.29%. , the content of vitamin C is 100-150 mg / 100 g, which ranks the highest among all kinds of citrus. In addition, it also contains vitamin B1, B2, B6, niacin, phosphorus, calcium, iron, etc., which have the functions o...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/001
Inventor 卢娟孙熹玉桂成陈剑成胡良先李建凡赵玉清宾耀梅陈莹莹
Owner 玉林市林业科学研究所
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