Unlock instant, AI-driven research and patent intelligence for your innovation.

Tetracycline separation and enrichment method for imidazolium ionic liquid double-aqueous phase system

An aqueous two-phase system and ionic liquid technology, which can be used in analytical materials, preparation of test samples, and material analysis by optical means. It can solve problems such as less separation of tetracycline antibiotics, and achieve strong selectivity and mild operating conditions. , the effect of structure can be designed

Inactive Publication Date: 2017-08-11
SOUTH CHINA UNIV OF TECH
View PDF2 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the extraction and separation of tetracycline antibiotics using ionic liquid / salt two-phase system

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Tetracycline separation and enrichment method for imidazolium ionic liquid double-aqueous phase system
  • Tetracycline separation and enrichment method for imidazolium ionic liquid double-aqueous phase system
  • Tetracycline separation and enrichment method for imidazolium ionic liquid double-aqueous phase system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Get 2.5mL mass concentration and be 50% [Bmim]Cl ionic liquid solution, be placed in centrifuge tube, then add 2.5mL mass concentration and be 50% K 2 HPO 4 Solution and 1mL of 1.2mg / mL tetracycline stock solution were shaken with a vortex shaker at 25°C to make them evenly mixed, shaken for 2min and placed in a centrifuge, set at 10000r / min, and centrifuged for 20min to separate the phases, then measure the upper and lower phases The volume of the volume and the concentration of tetracycline in the upper and lower phases, the volume of the upper and lower phases after extraction (V r =2.05) and the content of tetracycline in the upper and lower phases calculate the extraction rate of tetracycline to be 96%. Show that the method of the present embodiment has good extraction effect to tetracycline.

Embodiment 2

[0035] The operation method in this example is the same as that in Example 1, the only difference being that the imidazole ionic liquid two-phase system formed is different. In this example, the [Bmim]Cl ionic liquid is replaced by [Bmim]Br ionic liquid, and the rest Conditions remain the same.

[0036] Measure the volume of the upper and lower phases and the concentration of tetracycline in the upper and lower phases, and calculate the extraction rate of tetracycline from the volume of the upper and lower phases after extraction and the content of tetracycline in the upper and lower phases to be 94%. It shows that the aqueous two-phase system formed by [Bmim]Cl ionic liquid has a better extraction effect on tetracycline.

Embodiment 3

[0038] The method of operation in this embodiment is the same as that of Example 1, the only difference being the difference in the volume of the upper and lower phases of the formed ionic liquid two-phase system. In this embodiment, the addition of [Bmim]Cl ionic liquid solution is changed to 2 mL, K 2 HPO 4 The solution was changed to 3mL, and the rest of the conditions were unchanged.

[0039] Measure the volume of the upper and lower phases and the concentration of tetracycline in the upper and lower phases, from the volume of the upper and lower phases after extraction (V r =1.15) and the content of tetracycline in the upper and lower phases calculate the extraction rate of tetracycline to be 94.43%. It shows that the aqueous two-phase system formed by high volume ratio has a better extraction effect on tetracycline.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The invention discloses a tetracycline separation and enrichment method for an imidazolium ionic liquid double-aqueous phase system. The method comprises the following steps of 1, vibrating dipotassium phosphate solution, ionic liquid solution and TC stock solution and mixing uniformly to acquire mixed liquid; 2, putting the mixed liquid into a centrifugal machine to centrifuge or stand for splitting phases; and 3, testing a separation effect of TC. An extraction technology is optimized, the double-aqueous phase system formed when a wire tying length is controlled to be 30.52% and a volume ratio of upper to lower phases is controlled to be 2 at the normal temperature of below 25 DEG C has extraction efficiency of not less than 96% to tetracycline. According to the method provided by the invention, the tetracycline extracted and separated by using the imidazolium ionic liquid double-aqueous phase system, advantages of good solubleness, low energy consumption and moderate operation condition, non-pollution to environment, and simple operation are provided, and thus the method is an efficient and green tetracycline extraction method.

Description

technical field [0001] The invention relates to the field of separation of tetracyclines, in particular to a method for separating and enriching tetracyclines in an imidazole ionic liquid two-phase system. Background technique [0002] Tetracyclines (tetracyclines, TC) antibiotics are hydrogenated tetracene ring derivatives produced by Actinomycetes, which are effective against Gram-positive and negative bacteria, Rickettsia, viral viruses, Spirochetes and even protozoa. It has a good inhibitory effect and is a broad-spectrum antibiotic that can promote growth, prevent and treat intestinal infection in animals, and improve survival rate and feed conversion rate. Clinically, it is mainly used to treat infections caused by pathogens such as Gram-positive and negative bacteria, mycoplasma, chlamydia, rickettsia and spirochetes. It is a common drug for humans and animals, and its application is very extensive. However, due to the continuous pollution of the environment caused b...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N21/31
CPCG01N1/28G01N21/3103
Inventor 陈砺刘毅严宗诚
Owner SOUTH CHINA UNIV OF TECH