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A kind of pancreatic kininogenase and its preparation process

A technique for preparing pancreatic kininogenase and its preparation process, which is applied in the field of preparation of pancreatic kininogenase, can solve the problems that pancreatic kininogenase cannot be produced on a large scale, and achieves the advantages of small investment, short time consumption, and small loss of vitality. Effect

Active Publication Date: 2021-02-12
临沂新程金锣肉制品集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a kind of pancreatic kininogenase and its preparation process. The present invention first activates pancreatic kininogenase in pancreas and then separates it, which solves the problem that pancreatic kininogenase cannot be scaled up in the prior art. Difficulties in chemical production

Method used

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  • A kind of pancreatic kininogenase and its preparation process
  • A kind of pancreatic kininogenase and its preparation process

Examples

Experimental program
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Effect test

Embodiment 1

[0059] Embodiment 1 A preparation process of pancreatic kininogenase, the specific steps are as follows:

[0060] (1) Frozen pig pancreas thawed

[0061] Frozen pig pancreas is thawed to a semi-thawed state on a thawing rack in a sterile thawing room, ensuring that the central temperature of the pancreas is ≤0°C, the temperature in the thawing room is 18°C, and the relative air humidity is ≥80% RH;

[0062] The thawing room is disinfected with ozone, and tools and appliances such as thawing racks are sterilized with hot alkaline water or chlorine dioxide disinfectant.

[0063] Raw materials should avoid contamination during transshipment and should not come into contact with pollution sources such as the ground (pallets and other tools and utensils should be sterilized in advance).

[0064] (2) Minced

[0065] The thawed porcine pancreas is shredded in an all-in-one cutting and shredding machine, and the temperature of the porcine pancreas pulp during the shredding process i...

Embodiment 2

[0103] Embodiment 2 A preparation process of pancreatic kininogenase, the specific steps are as follows:

[0104] (1) Frozen pig pancreas thawed

[0105] Frozen pig pancreas is thawed to a semi-thawed state on a thawing rack in a sterile thawing room, ensuring that the central temperature of the pancreas is ≤0°C, the temperature in the thawing room is 16°C, and the relative air humidity is ≥80%RH;

[0106] The thawing room is disinfected with ozone, and tools and appliances such as thawing racks are sterilized with hot alkaline water or chlorine dioxide disinfectant.

[0107] Raw materials should avoid contamination during transshipment and should not come into contact with pollution sources such as the ground (pallets and other tools and utensils should be sterilized in advance).

[0108] (2) Minced

[0109] The thawed porcine pancreas is shredded in an all-in-one cutting and shredding machine, and the temperature of the porcine pancreas slurry during the shredding process ...

Embodiment 3

[0147] Embodiment 3 A preparation process of pancreatic kininogenase, the specific steps are as follows:

[0148] (1) Frozen pig pancreas thawed

[0149] Frozen pig pancreas is thawed to a semi-thawed state on a thawing rack in a sterile thawing room, ensuring that the central temperature of the pancreas is ≤0°C, the temperature in the thawing room is 20°C, and the relative air humidity is ≥80% RH;

[0150] The thawing room is disinfected with ozone, and tools and appliances such as thawing racks are sterilized with hot alkaline water or chlorine dioxide disinfectant.

[0151] Raw materials should avoid contamination during transshipment and should not come into contact with pollution sources such as the ground (pallets and other tools and utensils should be sterilized in advance).

[0152] (2) Minced

[0153] The thawed porcine pancreas is shredded in an all-in-one cutting and shredding machine, and the temperature of the porcine pancreas slurry during the shredding process...

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Abstract

The invention relates to a pancreatic kininogenase and a preparation process thereof. The preparation steps of the pancreatic kininogenase include: thawing frozen porcine pancreas, mincing, grinding, pretreatment, fiber separation, activation, activated pancreatic pulp filtration, 19 steps including alcohol precipitation, heavy phase filtration, granulation, etc., finally obtain pancreatic kininogenase. The present invention realizes the full activation of pancreatic kininogenase and elastase in the pancreas, creating the possibility for the further joint production of pancreatic kininogenase and pancreatic elastase; the activity of pancreatic elastase in the pancreatic kininogenase obtained in the present invention is very high High; the invention greatly improves the filtration speed and realizes industrialized production; the invention adopts the boiling drying method, which has small investment, short time consumption and low energy loss.

Description

technical field [0001] The invention relates to a pancreatic kininogenase and a preparation process thereof, belonging to the technical field of pancreatic kininogenase preparation. Background technique [0002] Pancreatic kininogenase, also known as pancreatic kallikrein and kallikrein, is ubiquitously present in the pancreas, submandibular gland and saliva of the human body, with the highest content in the pancreas. In the prior art, pancreatic kininogenase is a proteolytic enzyme extracted from porcine pancreas. It is composed of 18 amino acids and 4 sugars. It is an endoproteolytic enzyme with a molecular weight of 26800. , the enzyme exists in the form of a zymogen, namely prokallikrein. [0003] Pancreatic kininogenase currently on the market is basically extracted directly from the pancreas. First mince the pancreas, and then use acetate buffer to extract the pancreatic kininogenase from the pancreas. Fresh pancreas must first remove fat and connective tissue, unde...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/64
CPCC12N9/6445C12Y304/21008
Inventor 倪来学凌峰刘学生
Owner 临沂新程金锣肉制品集团有限公司
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