Protein of transcription factor NAC062D and application of encoding gene thereof in inhibiting seed germinating

Abstract

The invention relates to protein of a transcription factor NAC062D. The transcription factor is an active form after a transmembrane domain is removed from a membrane-associated transcription factor NAC062, an amino acid sequence is a sequence 1 in a sequence table, and a nucleotide sequence is a sequence 2 in the sequence table. A transgenic plant culture method of importing an encoding gene of the protein of the transcription factor NAC062D into a target plant is used for importing the encoding gene of the protein of the NAC062D into the target plant, so as to obtain the transgenic plant. The transgenic plant has the features that (1) under the normal condition, the transgenic plant is basically the same with the non-transgenic plant; (2) by adding 10mu M of inducing agent beta-estrodial (beta-E), the germinating rate of the transgenic plant is lower than the germinating rate of the non-transgenic plant. The function of the transgenic plant inhibiting the seed germinating is expressed by that the germinating rate of the transgenic plant is lower than the germinating rate of the target plant. The transcription factor NAC062D for regulating and controlling the germinating of the plant seeds and the encoding gene thereof are imported into arabidopsis thaliana (ecotype columbia), and the transgenic plant is obtained, so that the transcription factor can be applied to regulate and control the seed germinating.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to an active form NAC062D protein of a membrane-bound transcription factor NAC062 and the application of its coding gene in inhibiting seed germination. Background technique [0002] Monsanto researchers cloned the resistance gene (EPsPs gene) from petunia, and applied the plasmid-mediated transfer deoxyribonucleic acid (DNA) technology to transfer the EPsPs gene controlled by the 35s promoter in the petunia plasmid (caMv) Introduced into the soybean genome, and then bred glyphosate-resistant soybean varieties. This genetically modified soybean was approved by the U.S. Food and Drug Administration (FDA) in 1994 and became one of the earliest commercialized large-scale production of genetically modified crops. RR soybeans are highly tolerant to the non-selective herbicide Roundup (Rwndup) due to the herbicide glyphosate tolerance gene. Application of glyphosate herbicide in...

AI Technical Summary

Problems solved by technology

[0003] 1. It is said that genetically modified seeds cannot be saved, but only fresh genetically modified seeds can be bought, and genetically modified seeds are only sold exclusively by people who study genetically modified genes.

[0004] 2. For example, when soybeans need to be transformed into bean sprouts, genetic modification cannot be done, and non-GMO that can be transformed into bean sprouts must be purchased.

[0005] With the increasing environmental pollution, the greenhouse effect intensifies, the surface temperature rises, the temperature changes abnormally in four seasons, and the haze weather continues to occur, which seriously affects the growth and development of crops, and then affects food production.

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