A heat-resistant, acid-resistant, ethanol-resistant Saccharomyces cerevisiae and brewing starter
A technology of Saccharomyces cerevisiae and ethanol resistance, which is applied in the field of winemaking and microorganisms, can solve the problems that affect the smooth transfer of production in autumn, reduce fermentation activity, and reduce fermentation power, and achieve the improvement of quality and wine yield, high acidity, and time reduction. Effect
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Embodiment 1
[0025] The present embodiment makes wine-making starter according to the following steps:
[0026] A. Preparation of seed solution: sterilize the glutinous rice saccharification solution at 121°C for 20 minutes, and use it as a culture medium;
[0027] According to the inoculum amount of 10%, Saccharomyces cerevisiae was inoculated into the culture medium, and cultured at 32°C for 20 hours to obtain the primary seed liquid of Saccharomyces cerevisiae;
[0028] According to the inoculum amount of 10%, the primary seed liquid of Saccharomyces cerevisiae was inoculated into the culture medium, and cultured at 32° C. for 20 hours to obtain the secondary seed liquid of Saccharomyces cerevisiae;
[0029] According to the inoculation amount of 10%, the secondary seed liquid of Saccharomyces cerevisiae was inoculated into the culture medium, and cultured at 32° C. for 20 hours to obtain the tertiary seed liquid of Saccharomyces cerevisiae.
[0030] B. Moisturizing material: get 80 pa...
Embodiment 2
[0039] The present embodiment makes wine-making starter according to the following steps:
[0040] A. Preparation of seed solution: sterilize the glutinous rice saccharification solution at 121°C for 20 minutes, and use it as a culture medium;
[0041] According to the inoculum amount of 10%, Saccharomyces cerevisiae was inoculated into the culture medium, and cultured at 32°C for 20 hours to obtain the primary seed liquid of Saccharomyces cerevisiae;
[0042] According to the inoculation amount of 10%, inoculate the primary seed liquid of S. medium, cultured at 32°C for 20 hours to obtain the third grade seed liquid of Saccharomyces cerevisiae.
[0043] B. Moisturizing material: get 100 parts of wheat bran, 15 parts of corn flour and 10 parts of rice husk, add moisturizing material after mixing to make the water content reach 48%, and obtain the mixed material;
[0044] C. Steaming material: under the conditions of steam pressure 0.1Mpa and temperature 105°C, mix the mixtur...
Embodiment 3
[0053] The present embodiment makes wine-making starter according to the following steps:
[0054] A. Preparation of seed solution: sterilize the glutinous rice saccharification solution at 121°C for 20 minutes, and use it as a culture medium;
[0055] According to the inoculum amount of 10%, Saccharomyces cerevisiae was inoculated into the culture medium, and cultured at 32°C for 20 hours to obtain the primary seed liquid of Saccharomyces cerevisiae;
[0056] According to the inoculum amount of 10%, the primary seed liquid of Saccharomyces cerevisiae was inoculated into the culture medium, and cultured at 32° C. for 20 hours to obtain the secondary seed liquid of Saccharomyces cerevisiae;
[0057] According to the inoculation amount of 10%, the secondary seed liquid of Saccharomyces cerevisiae was inoculated into the culture medium, and cultured at 32° C. for 20 hours to obtain the tertiary seed liquid of Saccharomyces cerevisiae.
[0058] B. Moisturizing material: get 90 pa...
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