Method for detecting root colonization of plant growth-promoting rhizobacteria

Abstract

The invention relates to a method for detecting root colonization of plant growth-promoting rhizobacteria. The method comprises the following steps: 1) transferring wheat and performing liquid culture, namely transferring wheat seedlings cultured in a solid medium in a liquid MS medium, culturing on a horizontal table at the temperature of 25-28 DEG C at daytime with the illumination for 16 hours and at the temperature of 15-20 DEG C at night under dark conditions at a speed of 20-50 rmp for 24-48 hours, adding 1-3 ml of bacterium suspension, and continuously culturing for 5-10 days; 2) counting the quantity of the plant growth-promoting rhizobacteria, namely taking out the wheat seedlings, erasing the culture medium at the root by using sterile filter paper, and weighing; and placing in a tissue grinder, adding 2 ml of 1*PBS buffer solution, grinding into slurry, performing gradient dilution plate counting by adopting an LB solid medium, and calculating the colony formation unit quantity of fresh weight wheat roots per gram. According to the method disclosed by the invention, liquid shake culture is performed in a sterile environment, the culture conditions are stable and controllable, and the sampling method is standard and unified.

Description

technical field [0001] The invention belongs to the technical field of plant root system colonization measurement, and in particular relates to a method for detecting root system colonization of plant rhizosphere growth-promoting bacteria. Background technique [0002] Plant growth-promoting rhizobacteria (PGPR) can colonize the plant rhizosphere, improve the availability of plant nutrients, inhibit the growth of pathogenic bacteria or produce some special growth-promoting substances, promote plant seed germination and A type of bacteria involved in plant growth and development. A large number of studies have shown that the use of PGPR bacterial fertilizer can reduce the amount of chemical fertilizers by 20%-30%, reduce the amount of pesticides by more than 30%, and increase crop production by 10%-80%. The role in the development of sustainable agriculture has been paid more and more attention. [0003] The method for detecting PGPR colonization in the rhizosphere is common...

AI Technical Summary

Problems solved by technology

There are many factors affecting the colonization of PGPR in the rhizosphere detected by the solid-state matrix method, including the large differences in the matrix used in different laboratories, the influence of the matrix and other microorganisms in the air, the water content of the matrix is ​​difficult to control stably, and watering will cause bacteria to grow. Leaching loss, rhizosphere soil sampling methods are not easy to unify, etc., resulting in poor repeatability of experiments, and it is difficult to compare data obtained between different laboratories

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