Real-time fluorescence PCR immobilization kit of wheat dwarf bunt germ (Tilletia controversa kuhn) and its detection method

A technology of wheat dwarf smut and real-time fluorescence, applied in biochemical equipment and methods, botany equipment and methods, organic chemistry, etc., can solve problems such as non-standardization, low-temperature storage, errors, etc., and achieve strong specificity and sensitivity High, short detection time effect
CN1837364AInactive Publication Date: 2006-09-27CHONGQING UNIV +2
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
CHONGQING UNIV
Publication Date
2006-09-27
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a real-time fluorescent PCR fixed-phase agent box and detecting method of wheat short smut, which is characterized by the following: cloning the sequence character segment of relative end particle in the gene group DNA of Tilletia controversa Kuhn TCK according to the RM-PCR; developing the agent box based on specific primer, probe and real-time fluorescent PCR fixed-phase of differential segment sequence; detecting TCK teleutosorus or infective mycelium; producing result from preparing for 4 h.
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Description

technical field

[0001] The invention relates to an agricultural molecular biology, in particular to real-time fluorescent quantitative PCR detection of specific telomere-related sequences of Tilletia controversa Kühn (TCK), detection primer pairs, probes and solid-phase kits and It is suitable for the rapid detection technology of port inspection and quarantine and early diagnosis of field diseases. Background technique

[0002] Tilletia controversa Kühn (Tilletia controversa Kühn, TCK, hereinafter referred to as TCK), belongs to Basidiomycotina, Teliospora, Ustilagoles, Tilletia genus, is a disease of wheat dwarf smut (wheat dwarf bunt) disease) pathogenic bacteria. It is mainly distributed in the Americas, Europe, North Africa and West Asia, especially in the winter wheat regions of the seven states of Montana, Utah, Iowa, Colorado, Washington, and Oregon in the northwestern United States. This disease is the most harmful and extremely diffi...

Claims

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